TMH Lec 2

<p>Erwin Chargaff</p> Signup and view all the answers

<p>5' to 3'</p> Signup and view all the answers

<p>The DNA daughter strand that is synthesized continuously</p> Signup and view all the answers

<p>The DNA daughter strand that is synthesized discontinuously</p> Signup and view all the answers

<p>Short DNA molecules synthesized on the lagging strand</p> Signup and view all the answers

<p>DNA replication occurs at a very fast rate.</p> Signup and view all the answers

<p>C-DNA</p> Signup and view all the answers

<p>Maurice Wilkins</p> Signup and view all the answers

<p>Ribonucleic acid (RNA)</p> Signup and view all the answers

<p>All of the above</p> Signup and view all the answers

<p>Protein glycosylation</p> Signup and view all the answers

<p>Histidines, aspartates, and tyrosines</p> Signup and view all the answers

<p>A cell's complete set of hereditary information</p> Signup and view all the answers

<p>The code is flexible at the 2nd base</p> Signup and view all the answers

<p>To initiate translation in prokaryotes</p> Signup and view all the answers

<p>To regulate the activities of proteins</p> Signup and view all the answers

<p>It leads to swelling of the ankles and feet</p> Signup and view all the answers

<p>Bacteriophage Φ-X174</p> Signup and view all the answers

<p>All of the above</p> Signup and view all the answers

<p>23</p> Signup and view all the answers

<p>Instructions stored in a book</p> Signup and view all the answers

<p>2003</p> Signup and view all the answers

<p>Wide</p> Signup and view all the answers

<p>tRNAs have an anticodon loop that pairs with three basepairs (codon) in the mRNA.</p> Signup and view all the answers

<p>RNA polymerase catalyzes the formation of the phosphodiester bonds that link the nucleotides together to form a linear chain.</p> Signup and view all the answers

<p>Alternative splicing is the process of including particular exons within or excluding them from the final mRNA transcript.</p> Signup and view all the answers

<p>Structural proteins, catalytic proteins, transport proteins, regulatory proteins, and protective proteins.</p> Signup and view all the answers

<p>Agarose gels are prone to syneresis</p> Signup and view all the answers

<p>To visualize DNA migration during electrophoresis</p> Signup and view all the answers

<p>SYBR Safe</p> Signup and view all the answers

<p>Run the experiment in a temperature controlled environment</p> Signup and view all the answers

<p>Coomassie dye staining can be used for analysis by mass spectrometry or sequencing.</p> Signup and view all the answers

<p>Silver staining</p> Signup and view all the answers

<p>Isoelectric point</p> Signup and view all the answers

<p>By using carrier ampholytes and immobilized pH gradients together</p> Signup and view all the answers

<p>Ferdinand Frederic Reuss</p> Signup and view all the answers

<p>The molecular weight</p> Signup and view all the answers

<p>The movement of particles through a fluid due to the application of a spatially uniform electric field</p> Signup and view all the answers

<p>To separate and quantitate biopolymers such as proteins and nucleic acids</p> Signup and view all the answers

<p>$v_{eo} = \mu_{ep} + \mu_{eo}$</p> Signup and view all the answers

<p>Agarose</p> Signup and view all the answers

<p>Only analytes with a net charge can be separated.</p> Signup and view all the answers

<p>Uncharged solute ions</p> Signup and view all the answers

<p>The electrophoretic mobility of the analytes</p> Signup and view all the answers

<p>$vd = \mu_{ep}E$</p> Signup and view all the answers

<p>$\mu_{ep} = \frac{q},{f}$</p> Signup and view all the answers

<p>$FD = fvd = (6\pi \eta R)vd$</p> Signup and view all the answers

<p>Proteins are separated based on their size</p> Signup and view all the answers

<p>To align proteins in a sharp band</p> Signup and view all the answers

<p>To initiate the polymerization of acrylamide into chains</p> Signup and view all the answers

<p>To bind or react with the proteins and allow visualization</p> Signup and view all the answers

<p>The pH depends on the ratio of the two immobilines.</p> Signup and view all the answers

<p>The protein sample can be applied immediately.</p> Signup and view all the answers

<p>A technique to separate proteins based on both size and charge.</p> Signup and view all the answers

<p>It allows separation of proteins based on both size and charge.</p> Signup and view all the answers

<p>Protein maps.</p> Signup and view all the answers

<p>To compare protein expression patterns.</p> Signup and view all the answers

<p>The molecular weight</p> Signup and view all the answers

<p>Fundamental research</p> Signup and view all the answers

<p>$v_{eo} = \frac{q},{m}E$</p> Signup and view all the answers

<p>Small DNA fragments migrate faster than larger DNA fragments</p> Signup and view all the answers

<p>To align proteins in a sharp band at the starting line</p> Signup and view all the answers

<p>To compare protein expression between different samples</p> Signup and view all the answers

<p>Coomassie dye stains proteins in SDS-PAGE gels as blue bands</p> Signup and view all the answers

<p>Silver staining</p> Signup and view all the answers

<p>To stabilize the pH gradient</p> Signup and view all the answers

<p>They break down when the electric field is removed</p> Signup and view all the answers

<p>To reduce silver ions to metallic silver</p> Signup and view all the answers

<p>Proteins can be separated based on their isoelectric point (pI) using IPG.</p> Signup and view all the answers

<p>The pH gradient buffers are immobilized within the gel matrix.</p> Signup and view all the answers

<p>To compare protein maps between different samples.</p> Signup and view all the answers

<p>Agarose gels are prone to syneresis</p> Signup and view all the answers

<p>To visualize DNA migration during electrophoresis</p> Signup and view all the answers

<p>Q = I^2R</p> Signup and view all the answers

<p>To separate large DNA molecules</p> Signup and view all the answers

<p>The zeta potential is the potential at the slipping plane or the boundary of the diffuse layer and the bulk solution.</p> Signup and view all the answers

<p>µ = µep + µeo</p> Signup and view all the answers

<p>Positively-charged solute ions move faster.</p> Signup and view all the answers

<p>To act as a molecular sieve and retard the migrating molecules according to their size.</p> Signup and view all the answers

<p>$FD = (6\pi\eta R)vd$</p> Signup and view all the answers

<p>The coions in the fixed layer are present at a higher concentration, while those in the diffuse layer are present at a lower concentration.</p> Signup and view all the answers

<p>$\mu_{ep} = qE$</p> Signup and view all the answers

<p>$m^2V^{-1}s^{-1}$</p> Signup and view all the answers