10 Questions
What is one factor that the decision for the use of chemicals for electrophoresis depends on?
Size and quantity of proteins
Which buffer system is widely used and is also known as the 'Laemmli' system?
Tris-glycine
What is a limitation of the tris-glycine or 'Laemmli' gel buffer system?
Formation of disulfide bonds between proteins
When using precast gels, what is an advantage compared to handcast gels?
Reduction in time and effort for gel preparation
Which application is the Bis-Tris buffer system suitable for?
Mass spectrometry
What does the Bis-Tris buffer system have greater sensitivity in detecting compared to Tris-glycine?
Proteins
What pH range does the 'Laemmli' gel buffer system have for resolving?
~8.3-9.0
How can the limitation of disulfide bond formation in the gel buffer system be overcome?
By using buffers with lower pH
What type of gels are widely used in most labs as researchers follow a standard recipe for their preparation?
Handcast gels
Which type of gel is available as ready-to-use and can be used for several applications like SDS-PAGE and nondenatured protein analysis?
Ready-to-use precast-protein gels
Explore the concepts of biochemical similarities and differences in proteins, including how proteins determine structure and function, and how changes in DNA can lead to proteins with different functions. Learn about the importance of genetic diversity in natural selection and evolution.
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