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Questions and Answers
What is one factor that the decision for the use of chemicals for electrophoresis depends on?
What is one factor that the decision for the use of chemicals for electrophoresis depends on?
- Shape of proteins
- Color of proteins
- Size and quantity of proteins (correct)
- Smell of proteins
Which buffer system is widely used and is also known as the 'Laemmli' system?
Which buffer system is widely used and is also known as the 'Laemmli' system?
- Tris-glycine (correct)
- Disulfide
- Acrylamide
- Bis-Tris
What is a limitation of the tris-glycine or 'Laemmli' gel buffer system?
What is a limitation of the tris-glycine or 'Laemmli' gel buffer system?
- Enhanced stability of proteins in the gel
- Inability of proteins to move at all
- Formation of disulfide bonds between proteins (correct)
- No need for reducing agents
When using precast gels, what is an advantage compared to handcast gels?
When using precast gels, what is an advantage compared to handcast gels?
Which application is the Bis-Tris buffer system suitable for?
Which application is the Bis-Tris buffer system suitable for?
What does the Bis-Tris buffer system have greater sensitivity in detecting compared to Tris-glycine?
What does the Bis-Tris buffer system have greater sensitivity in detecting compared to Tris-glycine?
What pH range does the 'Laemmli' gel buffer system have for resolving?
What pH range does the 'Laemmli' gel buffer system have for resolving?
How can the limitation of disulfide bond formation in the gel buffer system be overcome?
How can the limitation of disulfide bond formation in the gel buffer system be overcome?
What type of gels are widely used in most labs as researchers follow a standard recipe for their preparation?
What type of gels are widely used in most labs as researchers follow a standard recipe for their preparation?
Which type of gel is available as ready-to-use and can be used for several applications like SDS-PAGE and nondenatured protein analysis?
Which type of gel is available as ready-to-use and can be used for several applications like SDS-PAGE and nondenatured protein analysis?
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