Protein Gel Electrophoresis: Biochemical Similarities and Differences
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Questions and Answers

What is one factor that the decision for the use of chemicals for electrophoresis depends on?

  • Shape of proteins
  • Color of proteins
  • Size and quantity of proteins (correct)
  • Smell of proteins
  • Which buffer system is widely used and is also known as the 'Laemmli' system?

  • Tris-glycine (correct)
  • Disulfide
  • Acrylamide
  • Bis-Tris
  • What is a limitation of the tris-glycine or 'Laemmli' gel buffer system?

  • Enhanced stability of proteins in the gel
  • Inability of proteins to move at all
  • Formation of disulfide bonds between proteins (correct)
  • No need for reducing agents
  • When using precast gels, what is an advantage compared to handcast gels?

    <p>Reduction in time and effort for gel preparation</p> Signup and view all the answers

    Which application is the Bis-Tris buffer system suitable for?

    <p>Mass spectrometry</p> Signup and view all the answers

    What does the Bis-Tris buffer system have greater sensitivity in detecting compared to Tris-glycine?

    <p>Proteins</p> Signup and view all the answers

    What pH range does the 'Laemmli' gel buffer system have for resolving?

    <p>~8.3-9.0</p> Signup and view all the answers

    How can the limitation of disulfide bond formation in the gel buffer system be overcome?

    <p>By using buffers with lower pH</p> Signup and view all the answers

    What type of gels are widely used in most labs as researchers follow a standard recipe for their preparation?

    <p>Handcast gels</p> Signup and view all the answers

    Which type of gel is available as ready-to-use and can be used for several applications like SDS-PAGE and nondenatured protein analysis?

    <p>Ready-to-use precast-protein gels</p> Signup and view all the answers

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