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Questions and Answers
In SDS-PAGE, what is the function of SDS (sodium dodecyl sulfate)?
In SDS-PAGE, what is the function of SDS (sodium dodecyl sulfate)?
- To protect proteins from degradation
- To stain the proteins
- To denature proteins and give them a uniform negative charge (correct)
- To solubilize the proteins
What does the term 'resolution' refer to in gel electrophoresis?
What does the term 'resolution' refer to in gel electrophoresis?
- The concentration of the gel
- The ability to distinguish between two closely spaced DNA fragments (correct)
- The voltage applied during electrophoresis
- The speed at which DNA fragments migrate
Why is it important to use a molecular weight marker in protein gel electrophoresis?
Why is it important to use a molecular weight marker in protein gel electrophoresis?
- To slow down the migration
- To stain the gel
- To determine the molecular weight of proteins (correct)
- To increase protein migration
What type of gel electrophoresis is typically used for RNA separation?
What type of gel electrophoresis is typically used for RNA separation?
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How can the accuracy of gel electrophoresis results be verified?
How can the accuracy of gel electrophoresis results be verified?
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Study Notes
Protein Electrophoresis
- SDS (sodium dodecyl sulfate) in SDS-PAGE denatures proteins and gives them a uniform negative charge.
- Resolution in gel electrophoresis refers to the ability to distinguish between two closely spaced DNA fragments.
- Molecular weight markers are used in protein gel electrophoresis to determine the molecular weight of proteins.
- Agarose gel electrophoresis with formaldehyde is typically used for RNA separation.
- The accuracy of gel electrophoresis results can be verified by repeating the experiment multiple times.
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Test your knowledge of protein electrophoresis, including SDS-PAGE, gel resolution, and molecular weight markers.
