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Questions and Answers
In SDS-PAGE, what is the function of SDS (sodium dodecyl sulfate)?
In SDS-PAGE, what is the function of SDS (sodium dodecyl sulfate)?
What does the term 'resolution' refer to in gel electrophoresis?
What does the term 'resolution' refer to in gel electrophoresis?
Why is it important to use a molecular weight marker in protein gel electrophoresis?
Why is it important to use a molecular weight marker in protein gel electrophoresis?
What type of gel electrophoresis is typically used for RNA separation?
What type of gel electrophoresis is typically used for RNA separation?
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How can the accuracy of gel electrophoresis results be verified?
How can the accuracy of gel electrophoresis results be verified?
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Study Notes
Protein Electrophoresis
- SDS (sodium dodecyl sulfate) in SDS-PAGE denatures proteins and gives them a uniform negative charge.
- Resolution in gel electrophoresis refers to the ability to distinguish between two closely spaced DNA fragments.
- Molecular weight markers are used in protein gel electrophoresis to determine the molecular weight of proteins.
- Agarose gel electrophoresis with formaldehyde is typically used for RNA separation.
- The accuracy of gel electrophoresis results can be verified by repeating the experiment multiple times.
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Description
Test your knowledge of protein electrophoresis, including SDS-PAGE, gel resolution, and molecular weight markers.
