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Protein Electrophoresis
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Protein Electrophoresis

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Questions and Answers

In SDS-PAGE, what is the function of SDS (sodium dodecyl sulfate)?

  • To protect proteins from degradation
  • To stain the proteins
  • To denature proteins and give them a uniform negative charge (correct)
  • To solubilize the proteins
  • What does the term 'resolution' refer to in gel electrophoresis?

  • The concentration of the gel
  • The ability to distinguish between two closely spaced DNA fragments (correct)
  • The voltage applied during electrophoresis
  • The speed at which DNA fragments migrate
  • Why is it important to use a molecular weight marker in protein gel electrophoresis?

  • To slow down the migration
  • To stain the gel
  • To determine the molecular weight of proteins (correct)
  • To increase protein migration
  • What type of gel electrophoresis is typically used for RNA separation?

    <p>Agarose gel electrophoresis with formaldehyde</p> Signup and view all the answers

    How can the accuracy of gel electrophoresis results be verified?

    <p>By repeating the experiment multiple times</p> Signup and view all the answers

    Study Notes

    Protein Electrophoresis

    • SDS (sodium dodecyl sulfate) in SDS-PAGE denatures proteins and gives them a uniform negative charge.
    • Resolution in gel electrophoresis refers to the ability to distinguish between two closely spaced DNA fragments.
    • Molecular weight markers are used in protein gel electrophoresis to determine the molecular weight of proteins.
    • Agarose gel electrophoresis with formaldehyde is typically used for RNA separation.
    • The accuracy of gel electrophoresis results can be verified by repeating the experiment multiple times.

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    Test your knowledge of protein electrophoresis, including SDS-PAGE, gel resolution, and molecular weight markers.

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