Protein Determination Titration Quiz
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Questions and Answers

The endpoint in the protein determination titration, in the presence of bromocresol green indicator, is when you obtain the ______ color.

  • Red
  • Yellow (correct)
  • Pink
  • Black
  • Which compound plays the role of a catalyst in the Kjeldahl method?

  • NaOH
  • K_2SO_4
  • (NH_4)_2SO_4
  • Cu_2SO_4
  • Which compound is titrated with HCl in the Kjeldahl method?

  • NH_4^+
  • NaOH
  • NH_3
  • H_2BO_3^- (correct)
  • Which equipment(s) must be calibrated before use?

    <p>(b) and (c)</p> Signup and view all the answers

    Degree Brix for a 12% sucrose solution means you have ______.

    <p>12g of sucrose in 100g of sample</p> Signup and view all the answers

    The refractive index (RI or n) depends on ______.

    <p>All these answers are correct</p> Signup and view all the answers

    Study Notes

    Protein Determination Titration

    • Endpoint color in protein determination titration with bromocresol green indicator is yellow.

    Kjedahl Method

    • Catalyst in the Kjedahl method is (NH₄)₂SO₄.
    • Compound titrated with HCl in Kjedahl method is NH₃.

    Equipment Calibration

    • Pycnometer, hydrometer, and refractometer must be calibrated before use.

    Sucrose Solution

    • 12% sucrose solution (degree Brix) is 1.2g sucrose in 100g of sample.

    Refractive Index

    • Refractive index (RI) depends on light wavelength, temperature, and solution concentration.

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    Description

    Test your knowledge on the procedures and principles of protein determination titration, including the use of indicators like bromocresol green. This quiz also covers the Kjedahl method and essential equipment calibration techniques. Understand the specifics of sucrose solutions and refractive index influences.

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