Protein Determination Titration Quiz
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Questions and Answers

The endpoint in the protein determination titration, in the presence of bromocresol green indicator, is when you obtain the ______ color.

  • Red
  • Yellow (correct)
  • Pink
  • Black

Which compound plays the role of a catalyst in the Kjeldahl method?

  • NaOH
  • K_2SO_4
  • (NH_4)_2SO_4
  • Cu_2SO_4

Which compound is titrated with HCl in the Kjeldahl method?

  • NH_4^+
  • NaOH
  • NH_3
  • H_2BO_3^- (correct)

Which equipment(s) must be calibrated before use?

<p>(b) and (c) (E)</p> Signup and view all the answers

Degree Brix for a 12% sucrose solution means you have ______.

<p>12g of sucrose in 100g of sample (C)</p> Signup and view all the answers

The refractive index (RI or n) depends on ______.

<p>All these answers are correct (B)</p> Signup and view all the answers

Flashcards

Protein Titration Endpoint

The endpoint in protein determination titration using bromocresol green indicator is when the solution turns black.

Kjeldahl Method Catalyst

The compound (NH₄)₂SO₄ acts as a catalyst in the Kjeldahl method for determining protein content.

Titrated Compound in Kjeldahl

In the Kjeldahl method, ammonia (NH₃) is the compound titrated with hydrochloric acid (HCl).

Equipment Calibration

Pycnometers and refractometers are examples of equipment that needs to be calibrated before use.

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Degree Brix

A Degree Brix of 12% in a sucrose solution means there are 12 grams of sucrose in 100 grams of the solution.

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Refractive Index Factors

The refractive index of a solution depends on the wavelength of light used, its temperature, and the concentration of the solution.

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What is the endpoint in protein determination titration using bromocresol green?

The endpoint in protein determination titration using bromocresol green indicator is when the solution turns black.

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What compound serves as a catalyst in the Kjeldahl method?

The compound (NH₄)₂SO₄ acts as a catalyst in the Kjeldahl method for determining protein content.

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Which compound is titrated with HCl in the Kjeldahl method?

In the Kjeldahl method, ammonia (NH₃) is the compound titrated with hydrochloric acid (HCl).

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Why do pycnometers and refractometers need calibration?

Pycnometers and refractometers are examples of equipment that needs to be calibrated before use to ensure accurate measurements.

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Study Notes

Protein Determination Titration

  • Endpoint color in protein determination titration with bromocresol green indicator is yellow.

Kjedahl Method

  • Catalyst in the Kjedahl method is (NH₄)₂SO₄.
  • Compound titrated with HCl in Kjedahl method is NH₃.

Equipment Calibration

  • Pycnometer, hydrometer, and refractometer must be calibrated before use.

Sucrose Solution

  • 12% sucrose solution (degree Brix) is 1.2g sucrose in 100g of sample.

Refractive Index

  • Refractive index (RI) depends on light wavelength, temperature, and solution concentration.

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Description

Test your knowledge on the procedures and principles of protein determination titration, including the use of indicators like bromocresol green. This quiz also covers the Kjedahl method and essential equipment calibration techniques. Understand the specifics of sucrose solutions and refractive index influences.

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