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Questions and Answers
What is the purpose of adding alkaline copper solution to the tubes?
What is the purpose of adding alkaline copper solution to the tubes?
- To denature the protein
- To set a zero point for the spectrophotometer
- To initiate the reduction of cupric ions (correct)
- To enhance the color development
What is the wavelength used to read the optical density in this experiment?
What is the wavelength used to read the optical density in this experiment?
- 740nm (correct)
- 450nm
- 520nm
- 650nm
What is the purpose of plotting the standard curve?
What is the purpose of plotting the standard curve?
- To determine the protein concentration in the test sample
- To match the OD of the test sample with the standard curve (correct)
- To determine the absorbance of the sample
- To identify the type of protein present in the sample
What is the function of reagent D in this experiment?
What is the function of reagent D in this experiment?
Why do too densely colored samples fail to give proper results using a spectrophotometer?
Why do too densely colored samples fail to give proper results using a spectrophotometer?
What is the name of the method used to determine the concentration of glucose in the given sample of blood?
What is the name of the method used to determine the concentration of glucose in the given sample of blood?
What is the purpose of spotting 10 µl of DNA standard on the plate?
What is the purpose of spotting 10 µl of DNA standard on the plate?
What is the role of phosphatase enzyme in biological molecules?
What is the role of phosphatase enzyme in biological molecules?
What is the principle behind fluorescence quantitation?
What is the principle behind fluorescence quantitation?
What is the purpose of using a transilluminator in fluorescence quantitation?
What is the purpose of using a transilluminator in fluorescence quantitation?
What is the role of DNA standards in fluorescence quantitation?
What is the role of DNA standards in fluorescence quantitation?
What is the purpose of calculating the amount of DNA in the extracted sample?
What is the purpose of calculating the amount of DNA in the extracted sample?
What is the purpose of adding liquid detergent in the DNA extraction process?
What is the purpose of adding liquid detergent in the DNA extraction process?
What is the function of the meat tenderizer/pineapple juice in the DNA extraction process?
What is the function of the meat tenderizer/pineapple juice in the DNA extraction process?
What is the purpose of adding ethanol to the DNA extraction process?
What is the purpose of adding ethanol to the DNA extraction process?
What is the purpose of using Ethidium bromide in the DNA quantitation process?
What is the purpose of using Ethidium bromide in the DNA quantitation process?
What is the purpose of using an agarose gel in the DNA quantitation process?
What is the purpose of using an agarose gel in the DNA quantitation process?
What is the purpose of centrifuging the DNA pellet at 3000 rpm for 10 minutes?
What is the purpose of centrifuging the DNA pellet at 3000 rpm for 10 minutes?