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Protein Concentration Lab

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Questions and Answers

What is the purpose of adding alkaline copper solution to the tubes?

To denature the protein
To set a zero point for the spectrophotometer
To initiate the reduction of cupric ions (correct)
To enhance the color development

What is the wavelength used to read the optical density in this experiment?

740nm (correct)
450nm
520nm
650nm

What is the purpose of plotting the standard curve?

To determine the protein concentration in the test sample
To match the OD of the test sample with the standard curve (correct)
To determine the absorbance of the sample
To identify the type of protein present in the sample

What is the function of reagent D in this experiment?

To develop the blue color (B) Signup and view all the answers

Why do too densely colored samples fail to give proper results using a spectrophotometer?

Because the sample absorbs too much light (D) Signup and view all the answers

What is the name of the method used to determine the concentration of glucose in the given sample of blood?

Folin-Wu method (D) Signup and view all the answers

What is the purpose of spotting 10 µl of DNA standard on the plate?

To calculate the amount of DNA in the extracted sample (D) Signup and view all the answers

What is the role of phosphatase enzyme in biological molecules?

It promotes both synthesis and breakdown of biological molecules (A) Signup and view all the answers

What is the principle behind fluorescence quantitation?

It compares the intensity of fluorescent light emitted by the unknown and standard samples (C) Signup and view all the answers

What is the purpose of using a transilluminator in fluorescence quantitation?

To compare the intensity of fluorescent light emitted by the unknown and standard samples (D) Signup and view all the answers

What is the role of DNA standards in fluorescence quantitation?

To provide a reference point for calculating the amount of DNA in the unknown sample (A) Signup and view all the answers

What is the purpose of calculating the amount of DNA in the extracted sample?

To determine the concentration of DNA in the sample (D) Signup and view all the answers

What is the purpose of adding liquid detergent in the DNA extraction process?

To break down the cell membrane (B) Signup and view all the answers

What is the function of the meat tenderizer/pineapple juice in the DNA extraction process?

To break down the protein in the pea mixture (B) Signup and view all the answers

What is the purpose of adding ethanol to the DNA extraction process?

To precipitate the DNA out of the solution (B) Signup and view all the answers

What is the purpose of using Ethidium bromide in the DNA quantitation process?

To increase the fluorescence of the DNA (A) Signup and view all the answers

What is the purpose of using an agarose gel in the DNA quantitation process?

To provide a surface for the DNA to bind to (C) Signup and view all the answers

What is the purpose of centrifuging the DNA pellet at 3000 rpm for 10 minutes?

To compact the DNA pellet (A) Signup and view all the answers

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