Protein Concentration Lab

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Questions and Answers

What is the purpose of adding alkaline copper solution to the tubes?

  • To denature the protein
  • To set a zero point for the spectrophotometer
  • To initiate the reduction of cupric ions (correct)
  • To enhance the color development

What is the wavelength used to read the optical density in this experiment?

  • 740nm (correct)
  • 450nm
  • 520nm
  • 650nm

What is the purpose of plotting the standard curve?

  • To determine the protein concentration in the test sample
  • To match the OD of the test sample with the standard curve (correct)
  • To determine the absorbance of the sample
  • To identify the type of protein present in the sample

What is the function of reagent D in this experiment?

<p>To develop the blue color (B)</p> Signup and view all the answers

Why do too densely colored samples fail to give proper results using a spectrophotometer?

<p>Because the sample absorbs too much light (D)</p> Signup and view all the answers

What is the name of the method used to determine the concentration of glucose in the given sample of blood?

<p>Folin-Wu method (D)</p> Signup and view all the answers

What is the purpose of spotting 10 µl of DNA standard on the plate?

<p>To calculate the amount of DNA in the extracted sample (D)</p> Signup and view all the answers

What is the role of phosphatase enzyme in biological molecules?

<p>It promotes both synthesis and breakdown of biological molecules (A)</p> Signup and view all the answers

What is the principle behind fluorescence quantitation?

<p>It compares the intensity of fluorescent light emitted by the unknown and standard samples (C)</p> Signup and view all the answers

What is the purpose of using a transilluminator in fluorescence quantitation?

<p>To compare the intensity of fluorescent light emitted by the unknown and standard samples (D)</p> Signup and view all the answers

What is the role of DNA standards in fluorescence quantitation?

<p>To provide a reference point for calculating the amount of DNA in the unknown sample (A)</p> Signup and view all the answers

What is the purpose of calculating the amount of DNA in the extracted sample?

<p>To determine the concentration of DNA in the sample (D)</p> Signup and view all the answers

What is the purpose of adding liquid detergent in the DNA extraction process?

<p>To break down the cell membrane (B)</p> Signup and view all the answers

What is the function of the meat tenderizer/pineapple juice in the DNA extraction process?

<p>To break down the protein in the pea mixture (B)</p> Signup and view all the answers

What is the purpose of adding ethanol to the DNA extraction process?

<p>To precipitate the DNA out of the solution (B)</p> Signup and view all the answers

What is the purpose of using Ethidium bromide in the DNA quantitation process?

<p>To increase the fluorescence of the DNA (A)</p> Signup and view all the answers

What is the purpose of using an agarose gel in the DNA quantitation process?

<p>To provide a surface for the DNA to bind to (C)</p> Signup and view all the answers

What is the purpose of centrifuging the DNA pellet at 3000 rpm for 10 minutes?

<p>To compact the DNA pellet (A)</p> Signup and view all the answers

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