18 Questions
What is the purpose of adding alkaline copper solution to the tubes?
To initiate the reduction of cupric ions
What is the wavelength used to read the optical density in this experiment?
740nm
What is the purpose of plotting the standard curve?
To match the OD of the test sample with the standard curve
What is the function of reagent D in this experiment?
To develop the blue color
Why do too densely colored samples fail to give proper results using a spectrophotometer?
Because the sample absorbs too much light
What is the name of the method used to determine the concentration of glucose in the given sample of blood?
Folin-Wu method
What is the purpose of spotting 10 µl of DNA standard on the plate?
To calculate the amount of DNA in the extracted sample
What is the role of phosphatase enzyme in biological molecules?
It promotes both synthesis and breakdown of biological molecules
What is the principle behind fluorescence quantitation?
It compares the intensity of fluorescent light emitted by the unknown and standard samples
What is the purpose of using a transilluminator in fluorescence quantitation?
To compare the intensity of fluorescent light emitted by the unknown and standard samples
What is the role of DNA standards in fluorescence quantitation?
To provide a reference point for calculating the amount of DNA in the unknown sample
What is the purpose of calculating the amount of DNA in the extracted sample?
To determine the concentration of DNA in the sample
What is the purpose of adding liquid detergent in the DNA extraction process?
To break down the cell membrane
What is the function of the meat tenderizer/pineapple juice in the DNA extraction process?
To break down the protein in the pea mixture
What is the purpose of adding ethanol to the DNA extraction process?
To precipitate the DNA out of the solution
What is the purpose of using Ethidium bromide in the DNA quantitation process?
To increase the fluorescence of the DNA
What is the purpose of using an agarose gel in the DNA quantitation process?
To provide a surface for the DNA to bind to
What is the purpose of centrifuging the DNA pellet at 3000 rpm for 10 minutes?
To compact the DNA pellet
Determine the protein concentration by following a series of steps involving alkaline copper solution, Folin reagent, and spectrophotometry. Record the OD values and plot the graph against different concentrations of protein.
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