5 Questions
What is the sensitive range of the Bradford assay when using 5 ml color reagent prepared in lab?
5 to 100 µg protein
What causes the visible color change in the Bradford assay?
Stabilization of the anionic form of the dye due to hydrophobic and ionic interactions with proteins
What is the principle behind the Bradford assay's measurement of protein concentration?
Formation of a complex between protein and a dye with constant extinction coefficient
What is a key advantage of the Bradford assay compared to the Lowry assay?
Faster speed and ability to retest out-of-range samples within minutes
For what purpose is the Bradford assay recommended, especially?
Determining protein content of cell fractions and assessing protein concentrations for gel electrophoresis
Learn about the considerations and advantages of using the Bradford protein assay, a fast and accurate method for determining protein content in biological samples. This quiz covers the practical aspects of the assay and its recommended use in biotechnology research and analysis.
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