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Questions and Answers
What is the sensitive range of the Bradford assay when using 5 ml color reagent prepared in lab?
What is the sensitive range of the Bradford assay when using 5 ml color reagent prepared in lab?
- 10 to 150 µg protein
- 1 to 50 µg protein
- 20 to 200 µg protein
- 5 to 100 µg protein (correct)
What causes the visible color change in the Bradford assay?
What causes the visible color change in the Bradford assay?
- Binding of Coomassie Brilliant Blue G-250 to any type of molecule
- Change in pH of the solution
- Stabilization of the anionic form of the dye due to hydrophobic and ionic interactions with proteins (correct)
- Presence of acidic solution
What is the principle behind the Bradford assay's measurement of protein concentration?
What is the principle behind the Bradford assay's measurement of protein concentration?
- Formation of a complex between protein and a dye with constant extinction coefficient (correct)
- Change in color due to protein presence
- Absorbance shift of Coomassie Brilliant Blue G-250 when binding to proteins
- Precipitation of proteins with a specific dye
What is a key advantage of the Bradford assay compared to the Lowry assay?
What is a key advantage of the Bradford assay compared to the Lowry assay?
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For what purpose is the Bradford assay recommended, especially?
For what purpose is the Bradford assay recommended, especially?
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