Bradford Protein Assay Considerations

10 to 150 µg protein

1 to 50 µg protein

20 to 200 µg protein

5 to 100 µg protein (correct)

Binding of Coomassie Brilliant Blue G-250 to any type of molecule

Change in pH of the solution

Stabilization of the anionic form of the dye due to hydrophobic and ionic interactions with proteins (correct)

Presence of acidic solution

Formation of a complex between protein and a dye with constant extinction coefficient (correct)

Change in color due to protein presence

Absorbance shift of Coomassie Brilliant Blue G-250 when binding to proteins

Precipitation of proteins with a specific dye

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