Podcast
Questions and Answers
What is the area on the enzyme where the substrate attaches called?
What is the area on the enzyme where the substrate attaches called?
What do we call the substance at the beginning of an enzymatic reaction on which an enzyme acts?
What do we call the substance at the beginning of an enzymatic reaction on which an enzyme acts?
What is the term used for an enzyme without its non-protein moiety?
What is the term used for an enzyme without its non-protein moiety?
Which type of cofactor is tightly bound to the apoenzyme by covalent bonds?
Which type of cofactor is tightly bound to the apoenzyme by covalent bonds?
Signup and view all the answers
What is the term for a non-protein chemical compound bound to an enzyme and required for catalysis?
What is the term for a non-protein chemical compound bound to an enzyme and required for catalysis?
Signup and view all the answers
What type of cofactor is derived from vitamins and loosely bound to the apoenzyme by non-covalent bonds?
What type of cofactor is derived from vitamins and loosely bound to the apoenzyme by non-covalent bonds?
Signup and view all the answers
What is the purpose of using 1-fluoro-2-4-dinitrobenzene (DNB) in sequencing polypeptides?
What is the purpose of using 1-fluoro-2-4-dinitrobenzene (DNB) in sequencing polypeptides?
Signup and view all the answers
In Edman Degradation, what does phenylisothiocyanate label?
In Edman Degradation, what does phenylisothiocyanate label?
Signup and view all the answers
What happens if Edman Degradation is repeated several times on a polypeptide?
What happens if Edman Degradation is repeated several times on a polypeptide?
Signup and view all the answers
Under acidic conditions, what happens to the bonds in a polypeptide chain when reacting with Sanger Reagent?
Under acidic conditions, what happens to the bonds in a polypeptide chain when reacting with Sanger Reagent?
Signup and view all the answers
What is the consequence of using 1-fluoro-2-4-dinitrobenzene (DNB) on the rest of the polypeptide chain?
What is the consequence of using 1-fluoro-2-4-dinitrobenzene (DNB) on the rest of the polypeptide chain?
Signup and view all the answers
What allows for repeated use of phenylisothiocyanate in Edman Degradation?
What allows for repeated use of phenylisothiocyanate in Edman Degradation?
Signup and view all the answers
Which environmental factor has the most significant impact on enzyme activity?
Which environmental factor has the most significant impact on enzyme activity?
Signup and view all the answers
What is the primary mechanism by which a competitive inhibitor slows down an enzymatic reaction?
What is the primary mechanism by which a competitive inhibitor slows down an enzymatic reaction?
Signup and view all the answers
What is the optimal pH range for most enzymes?
What is the optimal pH range for most enzymes?
Signup and view all the answers
How does substrate concentration affect the rate of an enzymatic reaction?
How does substrate concentration affect the rate of an enzymatic reaction?
Signup and view all the answers
What is the role of cofactors in enzymatic reactions?
What is the role of cofactors in enzymatic reactions?
Signup and view all the answers
How do competitive inhibitors affect the $K_m$ (Michaelis constant) of an enzymatic reaction?
How do competitive inhibitors affect the $K_m$ (Michaelis constant) of an enzymatic reaction?
Signup and view all the answers
What is the main difference between the lock-and-key model and the induced-fit model of enzyme action?
What is the main difference between the lock-and-key model and the induced-fit model of enzyme action?
Signup and view all the answers
How does an enzyme increase reaction rates according to the text?
How does an enzyme increase reaction rates according to the text?
Signup and view all the answers
What happens within the active site of an enzyme-substrate complex?
What happens within the active site of an enzyme-substrate complex?
Signup and view all the answers
What is one limitation of the lock-and-key model of enzyme action?
What is one limitation of the lock-and-key model of enzyme action?
Signup and view all the answers
How do enzymes achieve varying degrees of specificity for substrates?
How do enzymes achieve varying degrees of specificity for substrates?
Signup and view all the answers
What is the overall reaction for the conversion of substrate to product in enzyme-catalyzed reactions?
What is the overall reaction for the conversion of substrate to product in enzyme-catalyzed reactions?
Signup and view all the answers
Which of the following statements about non-competitive inhibitors is correct?
Which of the following statements about non-competitive inhibitors is correct?
Signup and view all the answers
Which of the following is a common naming convention for enzymes?
Which of the following is a common naming convention for enzymes?
Signup and view all the answers
Which of the following is NOT one of the six functional classes of enzymes according to the International Union of Biochemists (I.U.B.)?
Which of the following is NOT one of the six functional classes of enzymes according to the International Union of Biochemists (I.U.B.)?
Signup and view all the answers
What is the biochemical activity of oxidoreductases?
What is the biochemical activity of oxidoreductases?
Signup and view all the answers
Which of the following statements about competitive inhibitors is correct?
Which of the following statements about competitive inhibitors is correct?
Signup and view all the answers
Which of the following is an example of a common name used for a digestive enzyme?
Which of the following is an example of a common name used for a digestive enzyme?
Signup and view all the answers