Protein and Enzymes: Sequencing Polypeptides Quiz
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Questions and Answers

What is the term used to refer to the area on the enzyme where the substrate attaches?

  • Passive site
  • Inactive site
  • Active site (correct)
  • Empty site

What is the term for the substance at the beginning of an enzymatic reaction on which an enzyme acts?

  • Substrate (correct)
  • Apoenzyme
  • Prosthetic group
  • Coenzyme

Which type of enzyme is inactive without its non-protein moiety?

  • Prosthetic group
  • Apoenzyme (correct)
  • Coenzyme
  • Holoenzyme

What do we call a non-protein chemical compound tightly bound to an enzyme by covalent bonds?

<p>Prosthetic group (C)</p> Signup and view all the answers

What is the term for the special pocket or cleft in enzyme molecules where substrates bind?

<p>Active site (A)</p> Signup and view all the answers

What is the term for a group of enzymes that are active with their non-protein component?

<p>Holoenzyme (D)</p> Signup and view all the answers

What is the purpose of using 1-fluoro-2-4-dinitrobenzene in amino acid sequencing?

<p>To label the first amino acid in a polypeptide chain (D)</p> Signup and view all the answers

What is the limitation of using the Sanger reagent in amino acid sequencing?

<p>It destroys the rest of the polypeptide chain (A)</p> Signup and view all the answers

What is the role of the phenylisothiocyanate reagent in Edman degradation?

<p>To label the first amino acid in a polypeptide chain (A)</p> Signup and view all the answers

What is the advantage of using Edman degradation over the Sanger reagent?

<p>It does not destroy the rest of the polypeptide chain (C)</p> Signup and view all the answers

What is the significance of determining the amino acid sequence of a polypeptide?

<p>It determines the structure of the protein (D)</p> Signup and view all the answers

Who is credited with the development of the Sanger reagent?

<p>Fred Sanger (A)</p> Signup and view all the answers

What role do enzymes play in increasing reaction rates?

<p>Decreasing activation energy (D)</p> Signup and view all the answers

Which model of enzyme action involves a flexible active site?

<p>Induced Fit Model (A)</p> Signup and view all the answers

What does the Lock-and-Key Model of enzyme action state about the relationship between active sites and substrates?

<p>Active site has a rigid shape and only specific substrates fit (C)</p> Signup and view all the answers

Which type of enzymes show a greater range of substrate specificity according to the models discussed?

<p>Enzymes following the Induced Fit Model (D)</p> Signup and view all the answers

When a substrate fits into an enzyme's active site, which complex is formed?

<p>Enzyme-Substrate Complex (ES) (A)</p> Signup and view all the answers

What is the overarching effect of enzymes on reaction rates?

<p>Increase the rate of reaction (C)</p> Signup and view all the answers

What is the typical pH range at which most enzymes have an optimum pH?

<p>6 - 8 (near neutral) (A)</p> Signup and view all the answers

Which factor can denature enzymes due to high values?

<p>Extreme pH (B)</p> Signup and view all the answers

What occurs when an enzyme reaches saturation with substrate molecules?

<p>The reaction rate levels off (A)</p> Signup and view all the answers

Which of the following is an example of a cofactor for proper enzymatic activity?

<p>Minerals (e.g., zinc) (D)</p> Signup and view all the answers

What do competitive inhibitors do to enzymes?

<p>Prevent the active site from binding to the substrate (D)</p> Signup and view all the answers

In enzymatic reactions, what happens when the substrate concentration increases?

<p>The reaction rate increases until all enzyme molecules are in use (C)</p> Signup and view all the answers

What is the reagent that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green fluorescent sulfonamide adducts?

<p>Dansyl Chloride (B)</p> Signup and view all the answers

Which enzyme cleaves peptide bonds on the carboxyl side of aromatic amino acids like Phenylalanine, Tyrosine, and Tryptophan?

<p>Chymotrypsin (A)</p> Signup and view all the answers

What is the reagent that cleaves at tryptophan (Trp) residues?

<p>CNBr (A)</p> Signup and view all the answers

Which enzyme breaks down collagen in meat, making it tender?

<p>Papain (A)</p> Signup and view all the answers

What reacts with secondary amines and is more useful for samples containing brominated amino acids?

<p>Dansyl Chloride (B)</p> Signup and view all the answers

What cleaves at the peptide bond on the carboxyl side of Arginine and Lysine?

<p>Trpsin (A)</p> Signup and view all the answers

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