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Questions and Answers
What is the primary function of removing the phosphates from the DNA molecules?
What is the primary function of removing the phosphates from the DNA molecules?
- To prevent DNA ligation and keep the molecules linear (correct)
- To measure the presence of the labeled DNA through further steps
- To prepare the DNA for the next step in the process
- To replace the phosphate groups with radioactive ones
What is the purpose of radiolabeling the DNA molecules by replacing the phosphate groups?
What is the purpose of radiolabeling the DNA molecules by replacing the phosphate groups?
- To replace the radioactive phosphate groups with non-radioactive ones
- To prevent DNA ligation and keep the molecules linear
- To measure the presence of the labeled DNA through further steps (correct)
- To prepare the DNA for the next step in the process
Which enzyme is responsible for attaching the 5' end to the 3' end of DNA molecules?
Which enzyme is responsible for attaching the 5' end to the 3' end of DNA molecules?
- Polynucleotide kinase
- Restriction endonuclease
- DNA polymerase
- DNA ligase (correct)
Which enzyme is responsible for removing the phosphate groups from the DNA molecules?
Which enzyme is responsible for removing the phosphate groups from the DNA molecules?
Which step in the DNA preparation process is facilitated by the removal of the phosphate groups?
Which step in the DNA preparation process is facilitated by the removal of the phosphate groups?
Which part of the enzyme contains the nuclease activity?
Which part of the enzyme contains the nuclease activity?
What happens when the segment containing the first 323 amino acids is removed?
What happens when the segment containing the first 323 amino acids is removed?
Which enzyme activity is responsible for degrading DNA?
Which enzyme activity is responsible for degrading DNA?
What is the Klenow fragment?
What is the Klenow fragment?
What is the primary function of a primer in DNA replication or amplification?
What is the primary function of a primer in DNA replication or amplification?
Which enzyme is responsible for synthesizing a complementary DNA strand on a single-stranded template?
Which enzyme is responsible for synthesizing a complementary DNA strand on a single-stranded template?
What prevents the Klenow fragment from continuing the DNA synthesis once the nick is filled in?
What prevents the Klenow fragment from continuing the DNA synthesis once the nick is filled in?
Which enzyme is NOT involved in the process described involving the Klenow fragment?
Which enzyme is NOT involved in the process described involving the Klenow fragment?
What is the role of the Klenow fragment in DNA synthesis?
What is the role of the Klenow fragment in DNA synthesis?
Which component of the Klenow fragment's activity distinguishes it from other DNA polymerases?
Which component of the Klenow fragment's activity distinguishes it from other DNA polymerases?
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