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Nuclease Activity and Polymerase Function Quiz

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Nuclease Activity and Polymerase Function Quiz

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Questions and Answers

What is the primary function of removing the phosphates from the DNA molecules?

To prevent DNA ligation and keep the molecules linear (correct)

To measure the presence of the labeled DNA through further steps

To prepare the DNA for the next step in the process

To replace the phosphate groups with radioactive ones

What is the purpose of radiolabeling the DNA molecules by replacing the phosphate groups?

To replace the radioactive phosphate groups with non-radioactive ones

To prevent DNA ligation and keep the molecules linear

To measure the presence of the labeled DNA through further steps (correct)

To prepare the DNA for the next step in the process

Which enzyme is responsible for attaching the 5' end to the 3' end of DNA molecules?

Polynucleotide kinase

Restriction endonuclease

DNA polymerase

DNA ligase (correct)

Which enzyme is responsible for removing the phosphate groups from the DNA molecules?

Polynucleotide kinase Signup and view all the answers

Which step in the DNA preparation process is facilitated by the removal of the phosphate groups?

Radiolabeling Signup and view all the answers

Which part of the enzyme contains the nuclease activity?

The first 323 amino acids Signup and view all the answers

What happens when the segment containing the first 323 amino acids is removed?

The enzyme retains polymerase activity but loses nuclease activity Signup and view all the answers

Which enzyme activity is responsible for degrading DNA?

Nuclease Signup and view all the answers

What is the Klenow fragment?

A DNA polymerase lacking 5' to 3' exonuclease activity Signup and view all the answers

What is the primary function of a primer in DNA replication or amplification?

To provide a free 3' hydroxyl group for DNA polymerase to initiate synthesis Signup and view all the answers

Which enzyme is responsible for synthesizing a complementary DNA strand on a single-stranded template?

Klenow fragment Signup and view all the answers

What prevents the Klenow fragment from continuing the DNA synthesis once the nick is filled in?

Nuclease activity Signup and view all the answers

Which enzyme is NOT involved in the process described involving the Klenow fragment?

DNA ligase Signup and view all the answers

What is the role of the Klenow fragment in DNA synthesis?

Adding nucleotides to a growing DNA strand Signup and view all the answers

Which component of the Klenow fragment's activity distinguishes it from other DNA polymerases?

Lack of nuclease activity Signup and view all the answers