16 Questions
What is one of the advantages of recombinant DNA technology mentioned in the text?
Potential for mass-production
Which product is NOT mentioned as being treated with a protein in biotechnology?
Creutzfeld-Jakob disease
What is the role of protein in the treatment of anemia according to the text?
Assisting in increasing red blood cell count
Which disease is mentioned as potentially being prevented through the use of a protein-based vaccine?
Human papillomavirus
What cell type is associated with the expression choices mentioned in the text?
CHO cells
Why is biomanufacturing also known as bioprocessing?
It involves the use of living cells to produce desired products
Which major paradigm shifts kickstarted the 'big pharma' industry?
Dye-producing industry and chemical plants
Why were Sulfa-drugs initially inactive in-vitro but active in-vivo?
Lack of feasible methodology for in-vitro testing
Which company was not part of the major modern day 'big pharma' companies founded in the 1930s?
Roche
Why was the importance of biopharmaceuticals overlooked initially?
Absence of advanced technology for their manufacturing
What is a common problem with prokaryotic expression of eukaryotic proteins?
Rapid degradation by E.coli
Why are eukaryotic expression systems like yeast and mammalian cells preferred for expressing eukaryotic proteins?
Presence of posttranslational modification machinery
In protein purification, which chromatography technique separates proteins based on their charge?
Ion Exchange Chromatography
Which protein purification method utilizes metal complexes like Ni to capture specific proteins?
Glutathione s-transferase with glutathione
What is a common method used for quantifying proteins in a sample during purification?
Absorbance at 280nm
Which step in the protein expression and purification series involves centrifugation or instrumentation?
Purify
Learn about the challenges encountered when expressing eukaryotic proteins in prokaryotic systems, such as rapid degradation and non-functionality. Explore solutions like utilizing eukaryotic expression systems for successful protein production.
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