Problems with Prokaryotic Expression of Eukaryotic Proteins
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Questions and Answers

What is one of the advantages of recombinant DNA technology mentioned in the text?

  • Decreased control over the quantities produced
  • High cost of drugs
  • Increased risk of disease transmission
  • Potential for mass-production (correct)

Which product is NOT mentioned as being treated with a protein in biotechnology?

  • Hepatitis B virus
  • Heart attack
  • Creutzfeld-Jakob disease (correct)
  • Growth disorders

What is the role of protein in the treatment of anemia according to the text?

  • Assisting in increasing red blood cell count (correct)
  • Stimulating cell production
  • Regulating insulin levels
  • Boosting human growth hormone

Which disease is mentioned as potentially being prevented through the use of a protein-based vaccine?

<p>Human papillomavirus (C)</p> Signup and view all the answers

What cell type is associated with the expression choices mentioned in the text?

<p>CHO cells (B)</p> Signup and view all the answers

Why is biomanufacturing also known as bioprocessing?

<p>It involves the use of living cells to produce desired products (B)</p> Signup and view all the answers

Which major paradigm shifts kickstarted the 'big pharma' industry?

<p>Dye-producing industry and chemical plants (B)</p> Signup and view all the answers

Why were Sulfa-drugs initially inactive in-vitro but active in-vivo?

<p>Lack of feasible methodology for in-vitro testing (C)</p> Signup and view all the answers

Which company was not part of the major modern day 'big pharma' companies founded in the 1930s?

<p>Roche (C)</p> Signup and view all the answers

Why was the importance of biopharmaceuticals overlooked initially?

<p>Absence of advanced technology for their manufacturing (B)</p> Signup and view all the answers

What is a common problem with prokaryotic expression of eukaryotic proteins?

<p>Rapid degradation by E.coli (A)</p> Signup and view all the answers

Why are eukaryotic expression systems like yeast and mammalian cells preferred for expressing eukaryotic proteins?

<p>Presence of posttranslational modification machinery (C)</p> Signup and view all the answers

In protein purification, which chromatography technique separates proteins based on their charge?

<p>Ion Exchange Chromatography (A)</p> Signup and view all the answers

Which protein purification method utilizes metal complexes like Ni to capture specific proteins?

<p>Glutathione s-transferase with glutathione (C)</p> Signup and view all the answers

What is a common method used for quantifying proteins in a sample during purification?

<p>Absorbance at 280nm (A)</p> Signup and view all the answers

Which step in the protein expression and purification series involves centrifugation or instrumentation?

<p>Purify (A)</p> Signup and view all the answers

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