Problems with Prokaryotic Expression of Eukaryotic Proteins
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Questions and Answers

What is one of the advantages of recombinant DNA technology mentioned in the text?

  • Decreased control over the quantities produced
  • High cost of drugs
  • Increased risk of disease transmission
  • Potential for mass-production (correct)
  • Which product is NOT mentioned as being treated with a protein in biotechnology?

  • Hepatitis B virus
  • Heart attack
  • Creutzfeld-Jakob disease (correct)
  • Growth disorders
  • What is the role of protein in the treatment of anemia according to the text?

  • Assisting in increasing red blood cell count (correct)
  • Stimulating cell production
  • Regulating insulin levels
  • Boosting human growth hormone
  • Which disease is mentioned as potentially being prevented through the use of a protein-based vaccine?

    <p>Human papillomavirus</p> Signup and view all the answers

    What cell type is associated with the expression choices mentioned in the text?

    <p>CHO cells</p> Signup and view all the answers

    Why is biomanufacturing also known as bioprocessing?

    <p>It involves the use of living cells to produce desired products</p> Signup and view all the answers

    Which major paradigm shifts kickstarted the 'big pharma' industry?

    <p>Dye-producing industry and chemical plants</p> Signup and view all the answers

    Why were Sulfa-drugs initially inactive in-vitro but active in-vivo?

    <p>Lack of feasible methodology for in-vitro testing</p> Signup and view all the answers

    Which company was not part of the major modern day 'big pharma' companies founded in the 1930s?

    <p>Roche</p> Signup and view all the answers

    Why was the importance of biopharmaceuticals overlooked initially?

    <p>Absence of advanced technology for their manufacturing</p> Signup and view all the answers

    What is a common problem with prokaryotic expression of eukaryotic proteins?

    <p>Rapid degradation by E.coli</p> Signup and view all the answers

    Why are eukaryotic expression systems like yeast and mammalian cells preferred for expressing eukaryotic proteins?

    <p>Presence of posttranslational modification machinery</p> Signup and view all the answers

    In protein purification, which chromatography technique separates proteins based on their charge?

    <p>Ion Exchange Chromatography</p> Signup and view all the answers

    Which protein purification method utilizes metal complexes like Ni to capture specific proteins?

    <p>Glutathione s-transferase with glutathione</p> Signup and view all the answers

    What is a common method used for quantifying proteins in a sample during purification?

    <p>Absorbance at 280nm</p> Signup and view all the answers

    Which step in the protein expression and purification series involves centrifugation or instrumentation?

    <p>Purify</p> Signup and view all the answers

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