17 Questions
What type of test is used to assay the presence of an antigen?
Tube agglutination test
Which method is used for blood grouping to determine optimal proportion of antigen and antibody?
Tube agglutination test
In viral infection diagnosis, which method involves large, insoluble particles of antigens?
Slide agglutination test
What type of test is used to measure the level of antibody to a particulate antigen?
Tube agglutination test
Which test involves antigen-antibody reaction to form a visible precipitate?
Precipitation in agar with an electric field
What is the main purpose of serologic tests in medical diagnostics?
To measure the level of antibody to an antigen
What is the main difference between Radioimmunoassay (RIA) and Enzyme-linked immunosorbent assay (ELISA)?
RIA uses the detection of a radiolabeled product, while ELISA detects enzyme mediated color changes.
Which type of ELISA is used to test for the presence of a certain antigen?
Sandwich ELISA
What is the principle of ELISA based on?
Measurement of enzyme-substrate reaction color changes.
Which immunofluorescence testing technique involves the use of double-layer technique?
Indirect IF
What is the purpose of Radioimmunoassay (RIA) in viral infection diagnosis?
Detecting radiolabeled products in samples.
What is the principle behind the Antistreptolysin O test (ASOT)?
Neutralization of Streptolysin O toxin by patient's antibodies
What is the significance of an ASOT titer of ≥200 Todd's unit?
Diagnosis of active rheumatic fever
In Complement Fixation Tests (CFT), what happens if the tested serum is positive for antibodies?
Complement gets fixed by the antigen-antibody complex
What is the indicator system used in Complement Fixation Tests (CFT) to detect positive antibody reactions?
Sheep RBCs coated with their own antibodies
Which test detects the presence of Streptolysin O produced by Streptococci?
Antistreptolysin O test (ASOT)
What is the purpose of adding a control tube with no patient serum in both ASOT and CFT tests?
To act as a reference for antigen-antibody reactions
Explore the principles and procedures of precipitation tests in solution, precipitation in agar, and toxin-antitoxin neutralization tests like the Antistreptolysin O test (ASOT) used in Microbiology. Understand how these tests are conducted and how results are interpreted.
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