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Precipitation and Neutralization Tests in Microbiology

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Precipitation and Neutralization Tests in Microbiology

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Questions and Answers

What type of test is used to assay the presence of an antigen?

Slide agglutination test

Tube agglutination test (correct)

Precipitation test in solution

Blood grouping test

Which method is used for blood grouping to determine optimal proportion of antigen and antibody?

Tube agglutination test (correct)

Slide agglutination test

Precipitation in agar with an electric field

Serology Agglutination test

In viral infection diagnosis, which method involves large, insoluble particles of antigens?

Slide agglutination test (correct)

Precipitation in solution test

Serologic tests

Tube agglutination test

What type of test is used to measure the level of antibody to a particulate antigen?

Tube agglutination test Signup and view all the answers

Which test involves antigen-antibody reaction to form a visible precipitate?

Precipitation in agar with an electric field Signup and view all the answers

What is the main purpose of serologic tests in medical diagnostics?

To measure the level of antibody to an antigen Signup and view all the answers

What is the main difference between Radioimmunoassay (RIA) and Enzyme-linked immunosorbent assay (ELISA)?

RIA uses the detection of a radiolabeled product, while ELISA detects enzyme mediated color changes. Signup and view all the answers

Which type of ELISA is used to test for the presence of a certain antigen?

Sandwich ELISA Signup and view all the answers

What is the principle of ELISA based on?

Measurement of enzyme-substrate reaction color changes. Signup and view all the answers

Which immunofluorescence testing technique involves the use of double-layer technique?

Indirect IF Signup and view all the answers

What is the purpose of Radioimmunoassay (RIA) in viral infection diagnosis?

Detecting radiolabeled products in samples. Signup and view all the answers

What is the principle behind the Antistreptolysin O test (ASOT)?

Neutralization of Streptolysin O toxin by patient's antibodies Signup and view all the answers

What is the significance of an ASOT titer of ≥200 Todd's unit?

Diagnosis of active rheumatic fever Signup and view all the answers

In Complement Fixation Tests (CFT), what happens if the tested serum is positive for antibodies?

Complement gets fixed by the antigen-antibody complex Signup and view all the answers

What is the indicator system used in Complement Fixation Tests (CFT) to detect positive antibody reactions?

Sheep RBCs coated with their own antibodies Signup and view all the answers

Which test detects the presence of Streptolysin O produced by Streptococci?

Antistreptolysin O test (ASOT) Signup and view all the answers

What is the purpose of adding a control tube with no patient serum in both ASOT and CFT tests?

To act as a reference for antigen-antibody reactions Signup and view all the answers