Practical Biochemistry Blood Module Quiz
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Questions and Answers

What is the function of Potassium Oxalate as an anti-coagulant?

  • It reacts with calcium to form insoluble calcium oxalate and inhibits calcium participation in coagulation. (correct)
  • It reacts with potassium to form soluble potassium oxalate and aids in blood coagulation.
  • It reacts with sodium to form insoluble sodium oxalate and prevents clot formation.
  • It inhibits the actions of various enzymes involved in blood coagulation.
  • What is the role of Sodium Fluoride as an anti-coagulant?

  • It inhibits the actions of various enzymes involved in blood coagulation.
  • It reacts with calcium to form insoluble calcium fluoride and inhibits calcium participation in coagulation. (correct)
  • It reacts with sodium to form soluble sodium fluoride and aids in blood coagulation.
  • It reacts with potassium to form insoluble potassium fluoride and prevents clot formation.
  • What is the effect of Citrate as an anti-coagulant?

  • It reacts with calcium to form insoluble citrate and aids in blood coagulation.
  • Sodium or Potassium Citrate + Ca++ → Ca-Citrate (soluble, non-ionizable) (correct)
  • It inhibits the actions of various enzymes involved in blood coagulation.
  • It reacts with potassium to form soluble potassium citrate and prevents clot formation.
  • What is the function of EDTA as an anti-coagulant?

    <p>EDTA + Ca++ → soluble undissociable salt preventing liberation of Ca++</p> Signup and view all the answers

    What is the role of Heparin as an anti-coagulant?

    <p>Inhibits the actions of various enzymes involved in blood coagulation.</p> Signup and view all the answers

    Study Notes

    Introduction to Practical Biochemistry Blood Module

    • The blood module is a part of practical biochemistry
    • The module covers various topics related to blood biochemistry

    Composition of Blood

    • Plasma is blood without clotting factors, resulting in serum
    • Red Blood Cells (RBCs) are non-nucleated cells composed of:
      • 60-65% water
      • 35-40% solids, mainly metallo-chromoprotein (Hemoglobin)

    Cell Membrane Composition

    • Cell membrane or stroma is composed of:
      • Protein
      • Lipids, such as Lecithin, Cephalin, and Cholesterol
      • Semi-permeable membrane

    Preparation of Blood Samples

    • Whole Blood:
      • Mixing blood with water leads to hemolysis of RBCs
      • Used for analyzing Hb, Lactate, Pyruvate, and Ammonia levels
    • Serum:
      • Blood coagulates in the tube if left for a few minutes
      • Centrifugation separates serum from blood components

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    Description

    Test your knowledge of practical biochemistry with this quiz covering topics such as anti-coagulants, blood hemolysis, colorimetry, hemoglobin derivatives, CHO metabolism, diabetes mellitus, glucose tolerance, and HbA1C.

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