5 Questions
What is the function of Potassium Oxalate as an anti-coagulant?
It reacts with calcium to form insoluble calcium oxalate and inhibits calcium participation in coagulation.
What is the role of Sodium Fluoride as an anti-coagulant?
It reacts with calcium to form insoluble calcium fluoride and inhibits calcium participation in coagulation.
What is the effect of Citrate as an anti-coagulant?
Sodium or Potassium Citrate + Ca++ → Ca-Citrate (soluble, non-ionizable)
What is the function of EDTA as an anti-coagulant?
EDTA + Ca++ → soluble undissociable salt preventing liberation of Ca++
What is the role of Heparin as an anti-coagulant?
Inhibits the actions of various enzymes involved in blood coagulation.
Study Notes
Introduction to Practical Biochemistry Blood Module
- The blood module is a part of practical biochemistry
- The module covers various topics related to blood biochemistry
Composition of Blood
- Plasma is blood without clotting factors, resulting in serum
- Red Blood Cells (RBCs) are non-nucleated cells composed of:
- 60-65% water
- 35-40% solids, mainly metallo-chromoprotein (Hemoglobin)
Cell Membrane Composition
- Cell membrane or stroma is composed of:
- Protein
- Lipids, such as Lecithin, Cephalin, and Cholesterol
- Semi-permeable membrane
Preparation of Blood Samples
- Whole Blood:
- Mixing blood with water leads to hemolysis of RBCs
- Used for analyzing Hb, Lactate, Pyruvate, and Ammonia levels
- Serum:
- Blood coagulates in the tube if left for a few minutes
- Centrifugation separates serum from blood components
Test your knowledge of practical biochemistry with this quiz covering topics such as anti-coagulants, blood hemolysis, colorimetry, hemoglobin derivatives, CHO metabolism, diabetes mellitus, glucose tolerance, and HbA1C.
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