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Plant Transformation Methods Quiz

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Questions and Answers

What is the role of the Ti plasmid in Agrobacterium tumefaciens?

Initiating the infection of the plant at the wound site

Producing auxin and cytokinin for plant cell proliferation

Enabling the transfer of the T-DNA region into the plant cell (correct)

Breaking down opines and agropines as an energy source

Why does Agrobacterium tumefaciens cause crown gall formation in plants?

By inducing mutations in plant cell DNA

By inhibiting photosynthesis in the host plant

By releasing chemicals that attract it to the plant wound site (correct)

By producing toxins that damage plant cells

What is the unique aspect of gene transfer involving Agrobacterium species into plants?

It happens through an exchange of RNA molecules

It involves the transfer of bacterial genes into the plant genome (correct)

The transfer occurs intracellularly

The transfer leads to the production of toxins in the plant

What is the main reason for the interest in Agrobacterium tumefaciens from a biotechnological perspective?

Its unique capability to transfer bacterial genes into plants Signup and view all the answers

What do opines and agropines produced by plant cells during crown gall formation provide for Agrobacterium tumefaciens?

Nutrients for bacterial growth and energy source Signup and view all the answers

What is the main purpose of plant transformation as discussed in the text?

To understand plant biology and improve crop traits Signup and view all the answers

Which method is commonly used for the transformation of monocotyledonous plants?

Particle bombardment (biolistics) Signup and view all the answers

Why were other methods developed for transforming monocotyledonous plants besides using Agrobacterium?

Agrobacterium-based methods were ineffective on monocots Signup and view all the answers

Which organism is commonly used in Agrobacterium-based plant transformation?

Bacteria Signup and view all the answers

What is the significance of direct gene transfer methods like biolistics for plant transformation?

They allow transformation of monocotyledonous plants efficiently Signup and view all the answers