Plant Transformation Methods Quiz

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Questions and Answers

What is the role of the Ti plasmid in Agrobacterium tumefaciens?

  • Initiating the infection of the plant at the wound site
  • Producing auxin and cytokinin for plant cell proliferation
  • Enabling the transfer of the T-DNA region into the plant cell (correct)
  • Breaking down opines and agropines as an energy source

Why does Agrobacterium tumefaciens cause crown gall formation in plants?

  • By inducing mutations in plant cell DNA
  • By inhibiting photosynthesis in the host plant
  • By releasing chemicals that attract it to the plant wound site (correct)
  • By producing toxins that damage plant cells

What is the unique aspect of gene transfer involving Agrobacterium species into plants?

  • It happens through an exchange of RNA molecules
  • It involves the transfer of bacterial genes into the plant genome (correct)
  • The transfer occurs intracellularly
  • The transfer leads to the production of toxins in the plant

What is the main reason for the interest in Agrobacterium tumefaciens from a biotechnological perspective?

<p>Its unique capability to transfer bacterial genes into plants (C)</p> Signup and view all the answers

What do opines and agropines produced by plant cells during crown gall formation provide for Agrobacterium tumefaciens?

<p>Nutrients for bacterial growth and energy source (A)</p> Signup and view all the answers

What is the main purpose of plant transformation as discussed in the text?

<p>To understand plant biology and improve crop traits (A)</p> Signup and view all the answers

Which method is commonly used for the transformation of monocotyledonous plants?

<p>Particle bombardment (biolistics) (B)</p> Signup and view all the answers

Why were other methods developed for transforming monocotyledonous plants besides using Agrobacterium?

<p>Agrobacterium-based methods were ineffective on monocots (D)</p> Signup and view all the answers

Which organism is commonly used in Agrobacterium-based plant transformation?

<p>Bacteria (B)</p> Signup and view all the answers

What is the significance of direct gene transfer methods like biolistics for plant transformation?

<p>They allow transformation of monocotyledonous plants efficiently (D)</p> Signup and view all the answers

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