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Questions and Answers
What is the term used for the most commonly used plant transformation vectors due to their ability to replicate in both E. coli and Agrobacterium tumefaciens?
What is the term used for the most commonly used plant transformation vectors due to their ability to replicate in both E. coli and Agrobacterium tumefaciens?
- Replicative vectors
- Transgenic vectors
- Dual vectors
- Binary vectors (correct)
What are the key elements present in plant transformation vectors?
What are the key elements present in plant transformation vectors?
- Plasmid Selection, Plasmid Transformation, Transfer RNA (T-RNA) region
- Plasmid Integration, Plasmid Regeneration, Transfer DNA (T-DNA) region
- Plasmids Selection, Plasmids Replication, Transfer DNA (T-DNA) region (correct)
- Plasmids Insertion, Plasmids Replication, Transfer RNA (T-RNA) region
Which bacterium is commonly used to insert the recombinant DNA into plants during plant transformation?
Which bacterium is commonly used to insert the recombinant DNA into plants during plant transformation?
- Streptomyces coelicolor
- Agrobacterium tumefaciens (correct)
- Bacillus subtilis
- Escherichia coli
What is the function of the T-DNA region in plant transformation vectors?
What is the function of the T-DNA region in plant transformation vectors?
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What is the purpose of the Transfer DNA (T-DNA) region in plant transformation vectors?
What is the purpose of the Transfer DNA (T-DNA) region in plant transformation vectors?
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Study Notes
Plant Transformation Vectors
- Binary vectors are the most commonly used plant transformation vectors due to their ability to replicate in both E. coli and Agrobacterium tumefaciens.
Key Elements of Plant Transformation Vectors
- Origin of replication (ori) for replication in E. coli
- Selectable marker genes for selection of transformed cells
- Multiple cloning sites (MCS) for inserting the gene of interest
- T-DNA region for insertion into the plant genome
Plant Transformation Process
- Agrobacterium tumefaciens is commonly used to insert the recombinant DNA into plants during plant transformation.
Function of T-DNA Region
- The T-DNA region is responsible for the transfer of the recombinant DNA into the plant genome.
- The T-DNA region is flanked by left and right border sequences that are recognized by the Agrobacterium during the transformation process.
- The purpose of the Transfer DNA (T-DNA) region is to facilitate the insertion of the gene of interest into the plant genome.
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