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Questions and Answers
What is the term used for the most commonly used plant transformation vectors due to their ability to replicate in both E. coli and Agrobacterium tumefaciens?
What are the key elements present in plant transformation vectors?
Which bacterium is commonly used to insert the recombinant DNA into plants during plant transformation?
What is the function of the T-DNA region in plant transformation vectors?
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What is the purpose of the Transfer DNA (T-DNA) region in plant transformation vectors?
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Study Notes
Plant Transformation Vectors
- Binary vectors are the most commonly used plant transformation vectors due to their ability to replicate in both E. coli and Agrobacterium tumefaciens.
Key Elements of Plant Transformation Vectors
- Origin of replication (ori) for replication in E. coli
- Selectable marker genes for selection of transformed cells
- Multiple cloning sites (MCS) for inserting the gene of interest
- T-DNA region for insertion into the plant genome
Plant Transformation Process
- Agrobacterium tumefaciens is commonly used to insert the recombinant DNA into plants during plant transformation.
Function of T-DNA Region
- The T-DNA region is responsible for the transfer of the recombinant DNA into the plant genome.
- The T-DNA region is flanked by left and right border sequences that are recognized by the Agrobacterium during the transformation process.
- The purpose of the Transfer DNA (T-DNA) region is to facilitate the insertion of the gene of interest into the plant genome.
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Description
Test your knowledge of plant transformation technology with this quiz. Explore topics such as Ti and Ri plasmids, binary and co-integrated vector systems, viral vectors, genetic markers, cloning strategies, and gene transfer methods in plants, including direct DNA transfer and Agrobacterium mediated nuclear transformation. Gain insights into the essential techniques and tools used in plant genetic engineering.
