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15 Questions

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Plant Tissue Culture Lab Organization

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Questions and Answers

What is the function of the Hot Air Oven in the Washing room?

Drying glass wares and sterilizing metal tools (correct)

Storing stock solutions and chemicals

Boiling water to produce distilled water

Refrigerating and freezing stock solutions

What is the purpose of the Water distillation unit in the Washing room?

Producing distilled water free from minerals and impurities (correct)

Boiling water to create steam for sterilization

Sterilizing glass wares and metal tools

Storing stock solutions and chemicals

What equipment is typically found in the Media preparation room?

Hot Air Oven, Dispenser, High sensitivity balance

Hot plate with magnetic stirrer, PH meter, Weighing balance (correct)

Refrigerators, freezers, Microwave

Autoclave, PH meter, Weighing balance

What is the main function of the Culture room (aseptic transfer room)?

Facilitating aseptic transfer of cultures Signup and view all the answers

What is the purpose of the Green house (Net house) in the Plant tissue culture lab?

Providing controlled environment for plant growth Signup and view all the answers

What is the function of the Sulfuric acid (H2SO4) in the Washing room?

To sterilize glass wares and metal tools Signup and view all the answers

What is the purpose of the Dispenser in the Media preparation room?

To prepare culture media Signup and view all the answers

What is the main function of the Incubation room (Growth room) in the Plant tissue culture lab?

To provide a controlled environment for plant growth Signup and view all the answers

What is the purpose of the lead-lined sink in the Washing room?

To resist acids while washing glass wares Signup and view all the answers

What is the function of the Microwave in the Media preparation room?

To heat up solutions for media preparation Signup and view all the answers

What is the purpose of preparing stock solutions in plant tissue culture?

Save preparation time, conserve materials, reduce storage space, and improve accuracy of lower concentration solutions Signup and view all the answers

What is the molecular weight of 2,4-D in g/l?

221 g/l Signup and view all the answers

How should macronutrients be dissolved for stock solution preparation?

Separately in simple stock solutions to avoid precipitation Signup and view all the answers

Where is the best place to store macronutrient stock solutions?

In a refrigerator at 2-4°C Signup and view all the answers

What is the concentration of micronutrient stock solutions typically prepared as?

(100X) Signup and view all the answers