Plant Tissue Culture Lab Organization

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15 Questions

What is the function of the Hot Air Oven in the Washing room?

Drying glass wares and sterilizing metal tools

What is the purpose of the Water distillation unit in the Washing room?

Producing distilled water free from minerals and impurities

What equipment is typically found in the Media preparation room?

Hot plate with magnetic stirrer, PH meter, Weighing balance

What is the main function of the Culture room (aseptic transfer room)?

Facilitating aseptic transfer of cultures

What is the purpose of the Green house (Net house) in the Plant tissue culture lab?

Providing controlled environment for plant growth

What is the function of the Sulfuric acid (H2SO4) in the Washing room?

To sterilize glass wares and metal tools

What is the purpose of the Dispenser in the Media preparation room?

To prepare culture media

What is the main function of the Incubation room (Growth room) in the Plant tissue culture lab?

To provide a controlled environment for plant growth

What is the purpose of the lead-lined sink in the Washing room?

To resist acids while washing glass wares

What is the function of the Microwave in the Media preparation room?

To heat up solutions for media preparation

What is the purpose of preparing stock solutions in plant tissue culture?

Save preparation time, conserve materials, reduce storage space, and improve accuracy of lower concentration solutions

What is the molecular weight of 2,4-D in g/l?

221 g/l

How should macronutrients be dissolved for stock solution preparation?

Separately in simple stock solutions to avoid precipitation

Where is the best place to store macronutrient stock solutions?

In a refrigerator at 2-4°C

What is the concentration of micronutrient stock solutions typically prepared as?

(100X)

Test your knowledge on the organization of a plant tissue culture lab, including the different rooms and their functions such as the washing room, media preparation room, culture room, incubation room, green house, and data collection room.

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