Collecting and Preparing Fruit and Leaf Samples

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What was the effect of high quantities of methanolic extract of Curcuma longa on zebrafish embryos?

Had no adverse effect

Promoted healthy development

Caused mortality and developmental defects (correct)

Accelerated hatchability

What was the observed effect of phenolics-rich aqueous extracts of Cinnamon zeylanicum and Eugenia polyantha on zebrafish embryos at 48 hpf?

Enhanced heartbeat rates

No change in development

Increased survival rate

Severe toxicity, decreased survival rate, and organ abnormalities (correct)

What type of research design will be used in this study to evaluate the mutagenic and teratogenic effect of fruits and leaves extracted from Antidesma Bunius?

Case-control study design

Survey research design

Experimental research design (correct)

Quasi-experimental design

What will be used as a test organism for the Ames test in this study?

Salmonella typhimurium Signup and view all the answers

What will be used to prepare the extract for the study?

Fresh fruits and leaves of Antidesma bunius Signup and view all the answers

Where will the adult zebrafish be collected for the study?

Department of Science and Technology (DOST) Signup and view all the answers

Where will the extraction process of the fruits and leaves take place?

Bioassay Laboratory of Central Luzon State University Signup and view all the answers

What is the purpose of using Salmonella typhimurium in the experiment?

To determine the mutagenic effect of the fruit and leaf extracts Signup and view all the answers

What device is used to obtain the extract from the fruit and leaf samples?

Rotary evaporator Signup and view all the answers

What is the purpose of the teratogenic assay experiment?

To determine the teratogenic effects of the fruit and leaf extracts Signup and view all the answers

Where will the teratogenic assay experiment take place?

Bioassay Laboratory of Central Luzon State University Signup and view all the answers

What is the duration of the testing for mutagenic effects?

Several days Signup and view all the answers

What is the purpose of rinsing the fruits and leaves twice with distilled water?

To remove dirt and impurities from the surface Signup and view all the answers

What is the temperature and duration for oven-drying the fruits and leaves?

90°C for 3 days Signup and view all the answers

How many rounds of filtration are performed on the resulting extract?

Two rounds using Whatman 1 filter paper Signup and view all the answers

What is the concentration range of the Antidesma bunius fruit and leaf extract used for the mutagenic effects experiment?

0.3%, 0.6%, 1.25%, 2.5%, and 5% Signup and view all the answers

How is the powdered fruit and leaves stored after extraction?

In an amber bottle in an ultra-low freezer (-20°C) Signup and view all the answers

What is the purpose of soaking the powdered fruit and leaves in 95% methanol?

To extract bioactive compounds from the sample Signup and view all the answers

Collecting and Preparing Fruit and Leaf Samples

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