18 Questions
What was the effect of high quantities of methanolic extract of Curcuma longa on zebrafish embryos?
Caused mortality and developmental defects
What was the observed effect of phenolics-rich aqueous extracts of Cinnamon zeylanicum and Eugenia polyantha on zebrafish embryos at 48 hpf?
Severe toxicity, decreased survival rate, and organ abnormalities
What type of research design will be used in this study to evaluate the mutagenic and teratogenic effect of fruits and leaves extracted from Antidesma Bunius?
Experimental research design
What will be used as a test organism for the Ames test in this study?
Salmonella typhimurium
What will be used to prepare the extract for the study?
Fresh fruits and leaves of Antidesma bunius
Where will the adult zebrafish be collected for the study?
Department of Science and Technology (DOST)
Where will the extraction process of the fruits and leaves take place?
Bioassay Laboratory of Central Luzon State University
What is the purpose of using Salmonella typhimurium in the experiment?
To determine the mutagenic effect of the fruit and leaf extracts
What device is used to obtain the extract from the fruit and leaf samples?
Rotary evaporator
What is the purpose of the teratogenic assay experiment?
To determine the teratogenic effects of the fruit and leaf extracts
Where will the teratogenic assay experiment take place?
Bioassay Laboratory of Central Luzon State University
What is the duration of the testing for mutagenic effects?
Several days
What is the purpose of rinsing the fruits and leaves twice with distilled water?
To remove dirt and impurities from the surface
What is the temperature and duration for oven-drying the fruits and leaves?
90°C for 3 days
How many rounds of filtration are performed on the resulting extract?
Two rounds using Whatman 1 filter paper
What is the concentration range of the Antidesma bunius fruit and leaf extract used for the mutagenic effects experiment?
0.3%, 0.6%, 1.25%, 2.5%, and 5%
How is the powdered fruit and leaves stored after extraction?
In an amber bottle in an ultra-low freezer (-20°C)
What is the purpose of soaking the powdered fruit and leaves in 95% methanol?
To extract bioactive compounds from the sample
This quiz covers the procedures for collecting and preparing fruit and leaf samples, including authentication and extraction steps. Learn about the necessary steps for sample preparation and laboratory analysis.
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