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Questions and Answers
What is the stationary phase in paper chromatography?
What is the stationary phase in paper chromatography?
What is the stationary phase in thin layer chromatography?
What is the stationary phase in thin layer chromatography?
What is the mobile phase in gas chromatography?
What is the mobile phase in gas chromatography?
What is the mobile phase in liquid chromatography?
What is the mobile phase in liquid chromatography?
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Who discovered column chromatography?
Who discovered column chromatography?
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Study Notes
Stationary Phase in Chromatography
- In paper chromatography, the stationary phase is the cellulose paper, which acts as the medium where substances separate based on their affinity to the paper.
- In thin layer chromatography (TLC), the stationary phase is a thin layer of an adsorbent, commonly silica gel or alumina, applied to a plate, facilitating the separation of components based on their interactions with the stationary material.
Mobile Phase in Chromatography
- In gas chromatography, the mobile phase is an inert gas, typically helium or nitrogen, that carries the vaporized sample through the column for separation based on volatilities.
- In liquid chromatography, the mobile phase is a liquid solvent or a mixture of solvents that transports the sample through the stationary phase, allowing for separation based on solubility and interaction with the stationary material.
Discovery in Chromatography
- Column chromatography was discovered by the Russian botanist Mikhail Tswett in the early 20th century, who developed the technique for separating plant pigments.
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Description
Test your knowledge of planar chromatography techniques such as thin layer chromatography and paper chromatography with this quiz. Explore the separation of non-volatile mixtures and the use of stationary phases in the form of layers.
