Photosynthesis Overview and Chloroplast Anatomy

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Questions and Answers

What is the primary function of chloroplasts in plant cells?

  • Synthesize proteins
  • Transport nutrients
  • Synthesize glucose through photosynthesis (correct)
  • Store energy as fats

Which pigments are involved in absorbing light energy for photosynthesis?

  • Xanthophyll and zeaxanthin
  • Melanin and hemoglobin
  • Carotenoids and anthocyanins
  • Chlorophyll A, chlorophyll B, and carotenoids (correct)

What type of energy is utilized during the process of photosynthesis?

  • Chemical energy from water
  • Thermal energy from heating
  • Light energy from the sun (correct)
  • Kinetic energy from wind

What is the structure of chloroplasts?

<p>Double-membraned organelle (C)</p> Signup and view all the answers

Which statement accurately describes the role of chlorophyll in photosynthesis?

<p>Chlorophyll absorbs energy from sunlight (A)</p> Signup and view all the answers

Photosynthesis occurs in which type of cells?

<p>Plant cells (C)</p> Signup and view all the answers

Which of the following is NOT a component of chloroplasts involved in photosynthesis?

<p>Glucose (C)</p> Signup and view all the answers

What is the primary purpose of the Dark Reaction in photosynthesis?

<p>To fix carbon dioxide into glucose (A)</p> Signup and view all the answers

Which molecule is primarily regenerated during the Regeneration phase of the Calvin Cycle?

<p>RuBP (D)</p> Signup and view all the answers

In Cyclic Photophosphorylation, where do the energized electrons go after passing through the first electron transport chain?

<p>They return to the first ETC to generate ATP (D)</p> Signup and view all the answers

What is the result of the Reduction phase in the Calvin Cycle?

<p>Reduction of PGA to G3P (B)</p> Signup and view all the answers

Which enzyme plays a critical role in the Carboxylation step of the Calvin Cycle?

<p>RuBisCo (C)</p> Signup and view all the answers

What byproducts are produced during the Non-Cyclic Photophosphorylation process?

<p>Oxygen and NADP+ (B)</p> Signup and view all the answers

During the light reaction of photosynthesis, what is the immediate result of energized electrons moving through the electron transport chain?

<p>Formation of ATP (A)</p> Signup and view all the answers

What role does ATP play in the Calvin Cycle?

<p>It provides energy for the reduction of PGA (A)</p> Signup and view all the answers

What is the fate of the NADP+ produced during Non-Cyclic Photophosphorylation?

<p>It is converted back to NADPH (A)</p> Signup and view all the answers

What is the primary function of the thylakoid membrane in photosynthesis?

<p>Conversion of light energy into chemical energy (B)</p> Signup and view all the answers

Which component accumulates H+ ions during the process of photosynthesis?

<p>Thylakoid lumen (B)</p> Signup and view all the answers

What role does chlorophyll A play in the photosynthesis process?

<p>Absorbs light energy (D)</p> Signup and view all the answers

What structure is referred to as 'granum' in the context of photosynthesis?

<p>Stack of thylakoids (D)</p> Signup and view all the answers

Which of the following structures is involved in the Calvin Cycle?

<p>Stroma (A)</p> Signup and view all the answers

What happens to excited electrons in chlorophyll after they absorb photons?

<p>They are re-emitted as heat (D)</p> Signup and view all the answers

What is the role of the magnesium atom in chlorophyll?

<p>Absorbs photons effectively (A)</p> Signup and view all the answers

Which of the following is NOT a location associated with photosynthesis?

<p>Lysosome (D)</p> Signup and view all the answers

What is generated during the non-cyclic photophosphorylation process?

<p>ATP and NADPH (B)</p> Signup and view all the answers

Flashcards

What is photosynthesis?

Photosynthesis is a process used by plants to convert light energy from the sun into chemical energy in the form of glucose.

What is chlorophyll?

Chlorophyll is a pigment found in chloroplasts that absorbs light energy, particularly in the red and blue wavelengths.

What is a chloroplast?

Chloroplasts are organelles found in plant cells where photosynthesis takes place. They contain chlorophyll and other components necessary for the process.

What are Chlorophyll A, B, and carotenoids?

Chlorophyll A and B are the main pigments involved in absorbing light energy. Carotenoids are accessory pigments that absorb light in different wavelengths, extending the range of light used for photosynthesis.

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What are the two main stages of photosynthesis?

Photosynthesis takes place in two main stages: the light-dependent reactions and the light-independent reactions (Calvin cycle).

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What happens in the light-dependent reactions?

The light-dependent reactions use light energy to produce ATP and NADPH, which are energy carriers used in the Calvin cycle.

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What happens in the Calvin cycle?

The Calvin cycle uses the energy carriers (ATP and NADPH) produced in the light-dependent reactions to convert carbon dioxide into glucose.

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Stroma Lamellae

The site within chloroplasts where cyclic photophosphorylation takes place.

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Photophosphorylation

A process in photosynthesis where light energy is used to generate ATP.

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Cyclic Photophosphorylation

A type of photophosphorylation that utilizes a single photosystem (PSI) and produces ATP only. It involves electrons cycling back through the electron transport chain.

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Light Reactions

The first stage of photosynthesis where light energy is converted into chemical energy.

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Dark Reactions (Calvin Cycle)

The second stage of photosynthesis where CO2 is fixed into sugars. It occurs in the stroma of chloroplasts.

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RuBisCo

The enzyme responsible for catalyzing the first step in the Calvin Cycle, where CO2 is fixed onto RuBP.

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RuBP (Ribulose Bisphosphate)

The initial molecule that CO2 is attached to in the Calvin Cycle, starting the process of carbon fixation.

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G3P (Glyceraldehyde 3-Phosphate)

A 3-carbon compound generated in the Calvin Cycle, which serves as a building block for glucose.

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Regeneration

The process that regenerates RuBP, allowing the Calvin cycle to continue.

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Outer Membrane

The outermost layer of a chloroplast, regulating the passage of substances into and out of the organelle.

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Granum

Stacks of interconnected thylakoid membranes, forming the site of light-dependent reactions in photosynthesis.

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Thylakoid

Flattened, disc-shaped sacs within the chloroplast, responsible for capturing light energy and converting it to chemical energy.

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Thylakoid Membrane

The membrane surrounding the thylakoid, where the electron transport chain (ETC) takes place during non-cyclic photophosphorylation.

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Thylakoid Lumen

The space within the thylakoid, where the process of photolysis (water splitting) occurs, and protons (H+ ions) accumulate during the electron transport chain.

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Intermembrane Space

The space between the outer and inner membranes of a chloroplast.

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Inner Membrane

The inner most layer of a chloroplast, controlling the movement of substances in and out of the stroma.

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Stroma

The fluid-filled space within the chloroplast, where the Calvin cycle (light-independent reactions) takes place.

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Photons Excite Electrons

The process where light energy absorbed by chlorophyll molecules excites electrons, which then transfer to an electron transport chain.

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Study Notes

Photosynthesis Overview

  • Photosynthesis uses sunlight to create glucose from carbon dioxide and water.
  • Chlorophyll A, B, and carotenoids absorb sunlight's energy.
  • Excited electrons release energy, absorbed by Chlorophyll A (P680 and P700).
  • The process involves a porphyrin ring with a magnesium atom.

Chloroplast Anatomy

  • Photosynthesis takes place in chloroplasts, double-membraned organelles in plant cells.
  • Key structures include the outer membrane, granum (thylakoid stacks), thylakoids, thylakoid membrane (location of the ETC), thylakoid lumen, intermembrane space, inner membrane, stroma (Calvin Cycle location), and stroma lamellae (cyclic photophosphorylation location).

Steps of Photosynthesis

Light Reaction

  • Light energizes electrons in PSII, passed to an electron acceptor.
  • These electrons move through the ETC, creating 1.5 ATP.
  • Electrons are re-energized at PSI, then may either proceed through cyclic or non-cyclic pathways.
  • Cyclic phosphorylation generates 1 ATP.
  • Non-cyclic phosphorylation generates 1 ATP and NADPH for the Calvin Cycle.

Dark Reaction (Calvin Cycle)

  • Purpose: convert carbon dioxide into glucose (2 G3P).
  • Carboxylation: 6 carbon dioxide and 6 RuBP form 12 3-PGA.
  • Reduction: 12 ATP and 12 NADPH convert 12 3-PGA to 12 G3P.
  • Regeneration: 10 G3P are used to regenerate 6 RuBP, needed to start the Calvin Cycle.
  • Remaining 2 G3P are used to produce glucose.

Photolysis

  • During photolysis, water splits into hydrogen ions, electrons, and oxygen.
  • The hydrogen ions are used in creating NADPH.
  • Electrons replace those lost in photosystem II.
  • Oxygen is released as a byproduct.

Chemiosmosis

  • Hydrogen ions (H+) accumulate in the thylakoid lumen.
  • The hydrogen ion (H+) gradient generates ATP through ATP synthase.

Action Spectrum

  • Chloroplasts most effectively absorb red and blue light.
  • Green light is least effective for photosynthesis.

Alternatives to C3 Photosynthesis

  • C4 Photosynthesis: CO2 is moved to bundle sheath cells, minimizes photorespiration and water loss, and produces a 4-carbon intermediary compound needing an extra ATP.

  • CAM Photosynthesis: CO2 is fixed at night to minimize water loss and minimize photorespiration.

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