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Ch 4: Investigations

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51 Questions

What role does light play in photosynthesis based on the investigations?

Indispensable in driving photosynthetic processes

Which investigation emphasized the importance of carbon dioxide in photosynthesis?

Investigation 3

What was the purpose of covering a leaf with aluminum foil in one of the investigations?

To demonstrate the necessity of light in photosynthesis

Which pigment was highlighted for its pivotal role in photosynthesis?

Chlorophyll

What does the glowing splint test demonstrate in one of the investigations?

Production of oxygen

Which component was altered in the investigations to observe the effect on starch synthesis?

Carbon dioxide

What is the most critical factor for starch accumulation in the investigations?

Adequate light

Which substance led to the absence of starch accumulation in one of the investigations?

Sodium hydroxide

What was the outcome of exposing plants to sodium bicarbonate in an investigation?

Led to starch synthesis

How did scientists gain valuable insights into photosynthesis in the investigations?

By carefully manipulating variables

Why is it necessary to destarch a plant before conducting investigations related to photosynthesis?

To ensure accurate results by removing pre-existing starch that may interfere with the findings.

What is the purpose of the starch test in investigating photosynthetic activity?

To detect the presence of starch, a product of glucose synthesis during photosynthesis.

How does the staining with iodine solution help in the starch test?

It confirms the presence of starch in the leaf by turning it blue-black.

What role does chlorophyll extraction play in the starch test during investigations of photosynthesis?

It removes chlorophyll from the leaf to observe the starch presence more clearly.

Why is the destarching process conducted by subjecting the plant to darkness for 48 hours?

To allow the plant to utilize stored starch for metabolic activities.

Which step in the investigative process confirms the elimination of chlorophyll from the leaf in the starch test?

Staining with ethanol

What does the blue-black coloration observed in the leaf during the starch test indicate?

The presence of starch in the leaf.

During destarching, why is it essential to remove any pre-existing starch from the plant?

To ensure that detected starch is solely a result of photosynthetic activity.

What effect does subjecting a leaf to boiling water have on the subsequent starch test?

It aids in chlorophyll extraction, preparing the leaf for starch detection.

How does the ethanol treatment contribute to the accuracy of the starch test results?

It assists in extracting chlorophyll to ensure clear visualization of starch in the leaf.

What is the purpose of subjecting a plant to darkness for 48 hours in the destarching process?

To ensure accurate test results by removing pre-existing starch

Why is the starch test considered a reliable means of assessing photosynthetic activity?

It indicates the presence of starch synthesized during photosynthesis

What does the blue-black coloration in the leaf indicate in the context of the starch test?

Presence of starch

How does the subsequent staining with iodine solution contribute to the findings of the starch test?

It indicates the presence of starch in the leaf

What function does the ethanol treatment serve in the starch test process?

It extracts chlorophyll from the leaf

In the context of investigations on photosynthesis, what is the significance of desiccating the leaf after chlorophyll extraction?

To prepare the leaf for starch staining to detect photosynthetic activity

Which step before the starch test ensures that chlorophyll is no longer present in the leaf?

Treating the leaf with ethanol

How does the destarching process aid in confirming the occurrence of photosynthesis?

By decolorizing and desiccating the leaf

What role does starch synthesis play as a byproduct of photosynthesis in investigative procedures?

It serves as an indicator of photosynthetic activity

Which environmental factor is of utmost importance in the investigations related to photosynthesis?

Availability of carbon dioxide

What is the purpose of covering a portion of the leaf with aluminum foil in Investigation 2?

To prevent photosynthesis from occurring in that region

What conclusion can be drawn from the absence of starch accumulation in the foil-covered leaf in Investigation 2?

Light plays a crucial role in driving photosynthesis

Why was sodium hydroxide used in Investigation 3 in relation to carbon dioxide?

To absorb carbon dioxide in the experiment

What did the presence of starch accumulation in only the chlorophyll-containing regions of the variegated leaves demonstrate in Investigation 4?

The pivotal role of chlorophyll in photosynthesis

How does the glowing splint test confirm the production of oxygen during photosynthesis in Investigation 5?

By re-igniting or intensifying the burning of the splint

What does the absence of starch accumulation in the presence of CO2-absorbing sodium hydroxide prove in Investigation 3?

The indispensability of CO2 for photosynthetic carbon fixation

How does the starch test specifically demonstrate the role of chlorophyll in photosynthesis?

By showing starch accumulation in chlorophyll-containing areas

What evidence supports the claim that light drives photosynthetic processes?

The absence of starch accumulation in the foil-covered leaf

What is the primary function of sodium bicarbonate in Investigation 3?

To release carbon dioxide for photosynthesis

Why did the starch test only show accumulation in chlorophyll-containing regions in Investigation 4?

Chlorophyll's role as the primary photosynthetic pigment

Why subjecting a plant to darkness for 48 hours is imperative in the destarching process?

To remove any pre-existing starch to ensure accurate results

How does the subsequent staining with iodine solution help in the starch test?

Confirms the occurrence of photosynthesis

What purpose does covering a portion of the leaf with aluminum foil serve in Investigation 2?

To observe the effect of light blocking on starch synthesis

What evidence supports the destarching process aiding in confirming the occurrence of photosynthesis?

Conducting starch tests before and after destarching

What experimental variable was manipulated in Investigation 3 to ascertain the requirement of carbon dioxide for photosynthesis?

Sodium hydroxide solution

In the context of photosynthesis investigations, what is the significance of employing sensitive assays?

Detecting minute changes in photosynthesis

What does the re-ignition or intensified burning of a wooden splint in the glowing splint test prove in Investigation 5?

Presence of carbon dioxide

What does the absence of starch accumulation in the foil-covered leaf indicate in Investigation 2?

Light necessity for starch synthesis

Which experimental variable must be altered in investigations to observe the effect on starch synthesis, as described in the text?

Light intensity

What evidence from the investigations underscores the pivotal role of carbon dioxide in photosynthetic carbon fixation?

Sodium bicarbonate use

Based on the text, why was aluminum foil used to cover a leaf in Investigation 2?

To obstruct light access

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