Photosynthesis Fundamentals
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Questions and Answers

What is the independent variable in an experiment investigating the effect of temperature on the rate of photosynthesis?

Temperature

Describe how would you control the light intensity in an experiment investigating the effect of CO2 concentration on photosynthesis?

Keep the light source at a constant distance from the pondweed throughout the experiment.

Why is it important to use a suitable range for the independent variable in an experiment investigating the effect of a limiting factor on photosynthesis?

To ensure that the limiting factor is being investigated within its relevant range and that the results are meaningful.

What is the expected relationship between light intensity and the rate of photosynthesis?

<p>As light intensity increases, the rate of photosynthesis will increase until a maximum rate is reached, at which point it will plateau.</p> Signup and view all the answers

What are at least two methods of measuring the rate of photosynthesis?

<p>Counting bubbles of oxygen released by pondweed or measuring the increase in pH due to CO₂ absorption.</p> Signup and view all the answers

What is the limiting factor principle?

<p>Only one factor will be limiting the rate of a process at any given time, and this is the factor that is closest to its minimum value.</p> Signup and view all the answers

What is the current concentration of carbon dioxide in the atmosphere, and how does it compare to pre-industrial levels?

<p>The current atmospheric CO₂ concentration is over 400 ppm, which is significantly higher than the pre-industrial level of 270 ppm.</p> Signup and view all the answers

Explain how the carbon dioxide concentration in the atmosphere has changed since the late 18th century and why this change is significant.

<p>Carbon dioxide levels have risen by more than 50% due to human activities like burning fossil fuels, leading to significant environmental consequences such as climate change.</p> Signup and view all the answers

What is the primary goal of FACE experiments, as described in the text?

<p>FACE experiments aim to investigate the effects of increased CO₂ concentration on plant growth and ecosystems in a more natural setting.</p> Signup and view all the answers

Explain how FACE experiments are designed to specifically test the hypothesis that increased photosynthesis will moderate CO₂ levels.

<p>FACE experiments include both control plots receiving regular air and experimental plots receiving elevated CO₂. By comparing the growth and carbon uptake in both plots, researchers can assess whether the increased photosynthesis in the CO₂-enriched plots can offset the increased atmospheric CO₂.</p> Signup and view all the answers

Describe two potential consequences of the massive increase in atmospheric CO₂ concentration mentioned in the text.

<p>Two potential consequences are increased rates of photosynthesis and potential increases in plant growth and biomass.</p> Signup and view all the answers

What evidence supports the claim that increased CO₂ concentration can stimulate plant growth and biomass accumulation?

<p>The evidence cited is that there are already observed increases in photosynthesis and plant biomass due to rising CO₂ concentrations, and experiments with artificially increased CO₂ have shown similar results.</p> Signup and view all the answers

Why are CO₂ concentrations monitored inside the FACE experiment circles, and how is CO₂ released when it drops below 550 ppm?

<p>CO₂ concentrations are monitored to ensure consistency and maintain the targeted 550 ppm level. When this level drops, CO₂ is released on the upwind side of the circles to maintain the elevated concentration.</p> Signup and view all the answers

What is the purpose of the running solvent in chromatography?

<p>The running solvent carries the pigments up the paper and allows for separation based on different attractions between the pigments and the paper or solvent.</p> Signup and view all the answers

What does the Rf value in chromatography represent and what factors influence it?

<p>The <em>Rf</em> value represents the ratio of the distance traveled by a pigment to the distance traveled by the solvent. It is influenced by factors such as the chemical properties of the pigment, the solvent used, and the chromatography paper or thin layer plate.</p> Signup and view all the answers

Describe how solar energy is transformed into chemical energy during photosynthesis.

<p>Solar energy is absorbed by photosynthetic pigments, such as chlorophyll, and excites electrons in the molecule to a higher energy level. These excited electrons are then passed on to other molecules, releasing energy that is used to create chemical bonds in glucose or other carbon compounds.</p> Signup and view all the answers

What is the difference between an absorption spectrum and an action spectrum?

<p>An absorption spectrum shows the wavelengths of light absorbed by a pigment, whereas an action spectrum shows the rate of photosynthesis at different wavelengths of light.</p> Signup and view all the answers

How is the rate of photosynthesis measured in an experiment to obtain an action spectrum?

<p>The rate of photosynthesis can be measured by monitoring the rate of oxygen production or carbon dioxide consumption. The experiment can be conducted using LEDs at different wavelengths or color filters to vary the wavelength of light.</p> Signup and view all the answers

Explain how the wavelengths of light absorbed by chlorophyll affect the color of plants.

<p>Chlorophyll absorbs mainly red and blue wavelengths of light, while reflecting green light, which is why plants appear green.</p> Signup and view all the answers

What is the relationship between the energy of a photon and its wavelength?

<p>The energy of a photon is inversely proportional to its wavelength. Shorter wavelengths correspond to higher energy photons, while longer wavelengths correspond to lower energy photons.</p> Signup and view all the answers

Why are pigments such as chlorophyll important for photosynthesis?

<p>Pigments like chlorophyll absorb light energy and transfer it to other molecules, initiating the process of photosynthesis. They act as molecular antennas that capture light energy and convert it into chemical energy.</p> Signup and view all the answers

Explain the process of converting light energy to chemical energy during photosynthesis. What pigments are involved, and why is this step essential?

<p>Photosynthesis begins by capturing light energy using pigments, such as chlorophyll, which absorb specific wavelengths of light. This absorbed light energy is then converted into chemical energy, stored within the bonds of glucose molecules. This conversion is critical because it allows organisms to harness the energy from sunlight and use it for their metabolic processes.</p> Signup and view all the answers

Explain the importance of the splitting of water molecules in photosynthesis. What is the role of hydrogen and oxygen in this process?

<p>Splitting water molecules provides the necessary hydrogen atoms for converting carbon dioxide into glucose. The oxygen atoms released as a by-product of this splitting are also crucial for releasing energy from glucose during cellular respiration.</p> Signup and view all the answers

Describe the equation for photosynthesis using chemical formulas and words. Explain the role of each reactant and product.

<p>The equation for photosynthesis is: 6CO₂ + 12H₂O + light energy → C₆H₁₂O₆ + 6O₂ + 6H₂O. Carbon dioxide (CO₂) and water (H₂O) serve as reactants, along with light energy. The products of photosynthesis are glucose (C₆H₁₂O₆) and oxygen (O₂). Water is also a product, but the net change in water molecules from the reactants to products is zero.</p> Signup and view all the answers

Compare the ratio of atoms in a glucose molecule to the ratio of atoms in a carbon dioxide molecule. What is the significance of this difference in the context of photosynthesis?

<p>The ratio of atoms in a glucose molecule (C₆H₁₂O₆) is 1 carbon: 2 hydrogen: 1 oxygen, while the ratio in a carbon dioxide molecule (CO₂) is 1 carbon: 2 oxygen. The difference in ratios indicates that hydrogen atoms need to be added, and some oxygen atoms need to be removed from carbon dioxide to transform it into glucose. This is achieved by splitting water molecules and using the hydrogen atoms for the conversion process.</p> Signup and view all the answers

Explain how the products of photosynthesis are utilized by plants and other photosynthetic organisms. How do these products play a role in heterotrophic organisms?

<p>Plants use some of the glucose they produce as an energy source for their own growth and metabolic processes. Excess glucose is stored as starch for later use. The oxygen produced during photosynthesis is used for respiration in both plants and other organisms, as it is essential for releasing energy from glucose. Heterotrophic organisms, which cannot produce their own food, rely on consuming plants or other photosynthetic organisms to obtain glucose and oxygen, which they use for energy and growth.</p> Signup and view all the answers

Describe the process of chromatography as a technique for separating pigments from a leaf. Why is propanone a good solvent to use in this process?

<p>Chromatography involves separating pigments by their different solubility in a solvent. In the case of leaf pigments, the process starts by extracting pigments from a leaf using a solvent, such as propanone. Propanone is a good solvent because it dissolves the pigments in the leaf, allowing them to be separated by their different affinities for the solvent and the chromatography paper.</p> Signup and view all the answers

What are the three groups of organisms that carry out photosynthesis? How does the oxygen produced by these organisms benefit other organisms?

<p>The three main groups of organisms that carry out photosynthesis are plants, algae, and cyanobacteria. The oxygen released during photosynthesis is essential for aerobic respiration, which is the process by which most organisms, including humans, obtain energy from food. This oxygen is released into the atmosphere and is used for breathing and other processes.</p> Signup and view all the answers

Explain the role of light in photosynthesis. Why is light essential for this process?

<p>Light is the primary source of energy for photosynthesis. It is necessary to split water molecules, releasing hydrogen and oxygen, and providing the energy needed for the conversion of carbon dioxide into glucose. Without light, photosynthesis cannot occur.</p> Signup and view all the answers

Flashcards

CO₂ concentration increase

Forecasted to rise above double the pre-industrial level in the 21st century.

Photosynthesis rates

Increased rates due to higher CO₂ levels, which is often a limiting factor.

Plant biomass accumulation

Plants growing and storing carbon, reducing atmospheric CO₂.

FACE experiments

Research method using controlled carbon dioxide release in the environment.

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Ecosystem monitoring

Tracking variables in areas to predict ecosystem responses to increased CO₂.

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Photosynthesis

The process by which light energy is converted into chemical energy by plants, algae, and cyanobacteria.

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Light Energy to Chemical Energy

Light energy absorbed by pigments like chlorophyll is transformed into chemical energy during photosynthesis.

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Glucose Composition

A glucose molecule is composed of 1 carbon, 2 hydrogens, and 1 oxygen atom.

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Carbon Dioxide Conversion

Carbon dioxide is transformed into glucose by adding hydrogen and removing oxygen using energy from light.

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Water Splitting

Twelve water molecules are split to provide hydrogen needed for glucose synthesis during photosynthesis.

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Photosynthesis Equation

The overall equation for photosynthesis is: Carbon Dioxide + Water + Light -> Glucose + Oxygen.

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Oxygen Production

Oxygen is produced as a by-product of water splitting in photosynthesis; most is released into the atmosphere.

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Chromatography

A method used to separate leaf pigments to study photosynthesis by using solvents to extract pigments from leaves.

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Rf value

The ratio of the distance moved by a pigment to the distance moved by the solvent.

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Thin Layer Chromatography (TLC)

A technique used to separate mixtures of compounds based on their movement on a chromatography strip.

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Hydrophobic vs. Hydrophilic

Describes how pigments interact with the running solvent (hydrophobic) and the chromatography strip (hydrophilic).

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Absorption Spectrum

A graph showing which wavelengths of light are absorbed by a pigment.

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Action Spectrum

A graph showing the rate of photosynthesis at different wavelengths of light.

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Excited Electrons

Electrons that gain energy from photons, allowing them to move to a higher energy level.

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Wavelengths of Light

The distance between consecutive peaks of a wave, measured in nanometers (nm).

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Limiting Factor

The factor that is nearest to its minimum and limits the rate of a process like photosynthesis.

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Photosynthesis Factors

The three main factors affecting photosynthesis are temperature, light intensity, and carbon dioxide concentration.

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Independent Variable

The variable that is changed during an experiment to test its effects on photosynthesis.

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Control Variables

Variables that are kept constant to ensure a fair test during an experiment.

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Oxygen Production Measurement

A method of measuring the rate of photosynthesis by observing the amount of oxygen produced over time.

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Temperature Experiment Setup

Using a thermostatically controlled water bath to vary temperature in a photosynthesis experiment.

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Light Intensity Variation

Changing the distance of a light source to examine its impact on the rate of photosynthesis.

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Carbon Dioxide Enrichment

Increasing carbon dioxide levels in an experiment to study its effects on photosynthesis.

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Study Notes

Photosynthesis

  • Photosynthesis converts light energy into chemical energy stored in carbon compounds.
  • Chlorophyll and other pigments absorb light energy.
  • The equation for photosynthesis is: carbon dioxide + water + light → glucose + oxygen.
  • Oxygen is a byproduct of splitting water to release hydrogen.
  • Photosynthesis is carried out by plants, algae, and cyanobacteria.
  • Oxygen produced during photosynthesis is released into the environment.
  • The process of transferring light energy to chemical energy is dependent upon light being available.

Carbon Dioxide to Glucose

  • Glucose molecules have a carbon-to-hydrogen-to-oxygen ratio of 1:2:1.
  • Carbon dioxide molecules have a carbon-to-oxygen ratio of 1:2.
  • To convert carbon dioxide into glucose, oxygen atoms must be removed, and hydrogen added.
  • Splitting water molecules provides hydrogen.
  • 12 water molecules are required for every glucose molecule formed.

Photosynthesis Equation

  • Carbon dioxide + water + light energy → glucose + oxygen.
  • The equation summarizes the process of photosynthesis wherein plants use carbon dioxide and water in the presence of light energy to produce glucose and oxygen.

Chromatography

  • Chromatography is used to separate pigments (colored substances) from plants.
  • Leaf pigments are ground with sand and propanone to extract the pigments, and the solution is transferred to a small spot on a TLC strip (thin layer chromatography strip).
  • The strip is suspended in a solvent, which moves up the paper carrying the pigments at different speeds.
  • Pigment identification is based on their Rf value, which is the distance moved by a spot divided by the distance moved by the solvent.
  • Different pigments have different Rf values in the solvent system.

Wavelengths of Light in Photosynthesis

  • Plants absorb light in the 400 to 700 nm wavelength range.
  • Violet light has the shortest wavelength, and red the longest.
  • Shorter wavelengths have more energy.
  • Chlorophyll absorbs red and blue light most effectively, while reflecting green light most. This is why plants appear green.

Photosynthetic Absorption and Action Spectra

  • The absorption spectrum shows the wavelengths of light absorbed by different pigments (chlorophyll). It relates to the wavelengths of light that cause pigment electrons to jump energy levels.
  • The action spectrum shows the rate of photosynthesis at different wavelengths.
  • The action spectrum is generally similar to the absorption spectrum, showing that Chlorophyll a and b effectively absorb and use light from the red/blue wavelengths. Other accessory pigments increase effective use of photons and light absorption in the lower visible light spectrum (green region).
  • Accessory pigments extend the range of wavelengths used in photosynthesis, enabling a broader spectrum of light to be used.

Limiting Factors in Photosynthesis

  • Limiting factors in photosynthesis include temperature, light intensity, and carbon dioxide concentration.
  • Experiments are designed to investigate the effect of one limiting factor, while keeping the other two constant.
  • Temperature, light intensity, and CO2 concentration are manipulated as the independent variables.
  • The rate of photosynthesis is the dependent variable being measured.

Carbon Dioxide Enrichment Experiments

  • Carbon dioxide concentrations have significantly increased since the late 18th century.
  • Experiments are conducted in greenhouses to increase CO2 concentrations.
  • "Free air carbon dioxide enrichment" (FACE) experiments monitor the effects of increased CO2 concentrations on plants in natural environments.
  • Results of these experiments help predict future photosynthesis rates and plant growth.
  • Increased CO2 can lead to higher growth and biomasses, though there are other factors that impact the actual increase.

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C1.3 Photosynthesis PDF

Description

Explore the essential processes of photosynthesis, where light energy is converted into chemical energy stored in glucose. This quiz covers key aspects including the role of chlorophyll, the chemical equation for photosynthesis, and the significance of oxygen production. Test your understanding of how carbon dioxide and water contribute to forming glucose.

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