The role of light in photosynthesis practical

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Questions and Answers

What is the purpose of wrapping a part of the leaf with aluminium foil in the experiment?

  • To enhance the light absorption of the leaf.
  • To block exposure to light, acting as a control. (correct)
  • To provide nourishment to the plant.
  • To prevent water loss from the leaf.

What is the function of iodine solution in the experiment?

  • To nourish the plant.
  • To measure light intensity.
  • To increase photosynthesis rates.
  • To test for the presence of starch. (correct)

What is indicated by the presence of starch in the leaf after the experiment?

  • Photosynthesis occurred where light was available. (correct)
  • The control set-up was affected by external factors.
  • The plant was unhealthy before the experiment.
  • Light was not necessary for photosynthesis.

Why is a control set-up important in this experiment?

<p>It ensures external factors do not affect the results. (A)</p> Signup and view all the answers

What safety precautions must be taken when using alcohol in the experiment?

<p>Ensure adequate ventilation and avoid naked flames. (A)</p> Signup and view all the answers

What is the significance of using a destarched plant in this experiment?

<p>To provide a clean starting point without stored starch. (D)</p> Signup and view all the answers

What is the purpose of using a destarched plant from the beginning of this experiment?

<p>To ensure any starch detected in the experiment is produced by the plant during the experiment.</p> Signup and view all the answers

What part of the plant is used as a 'control' in this experiment?

<p>The part of the plant that is wrapped in aluminium foil.</p> Signup and view all the answers

In this experiment, what is the independent variable?

<p>Presence of light.</p> Signup and view all the answers

What are two important factors that are kept constant during this experiment?

<p>Supply of oxygen (B), Supply of water (D)</p> Signup and view all the answers

What would you expect to observe when you test the part of the leaf that was exposed to light?

<p>A blue-black color, indicating the presence of starch (B)</p> Signup and view all the answers

What would you expect to observe after testing the part of the leaf that was covered with aluminium foil?

<p>No change in color when tested with iodine (A)</p> Signup and view all the answers

What would be the expected result, if the experiment was conducted in complete darkness instead of bright light?

<p>No starch production in any part of the leaf (C)</p> Signup and view all the answers

What would be a reasonable conclusion to draw from the experiment, if both the covered and uncovered parts of the leaf show the same results?

<p>The experiment might need to be repeated to investigate why the expected results were not observed.</p> Signup and view all the answers

If the experiment was repeated with red light, instead of bright white light, what might be the difference in the results?

<p>Starch production might be less, because red light is not as effective at driving photosynthesis as white light.</p> Signup and view all the answers

Flashcards

Photosynthesis

The process plants use to make food using sunlight, water, and carbon dioxide.

Control setup

A setup that is identical to the experimental setup, except that the factor being tested is absent.

Iodine test

A test using iodine solution to check for the presence of starch.

Destarch plant

A plant that has had its starch removed, often by leaving it in the dark.

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Experimental setup

The setup in which the factor being tested is present.

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Light exposure

Part of a leaf that is exposed to light.

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Control set-up procedure

The procedure to ensure that any results are caused by the tested factor and not other factors.

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Starch

A nutrient that plants store as energy.

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Initial iodine test

The first iodine test conducted before any light exposure to establish a baseline for starch presence.

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Light-exposed area

The part of the leaf directly exposed to bright light in the experiment.

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Four-hour light exposure

The duration for which the plant is left in bright light for photosynthesis to occur.

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Aluminium foil removal

The step where the foil is removed from the leaf to expose the covered area to light.

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Final iodine test

The iodine test performed again after the light exposure to compare starch levels in different parts of the leaf.

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Starch production in light-exposed areas

The expected result of starch being present in the light-exposed part of the leaf.

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Control setup significance

The control setup helps demonstrate the importance of light in photosynthesis, as it shows no starch production in the covered area.

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Experiment duration (four hours)

The four-hour period allows sufficient time for starch production during photosynthesis.

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Same leaf used for both areas

Using the same leaf for the control and experimental setup ensures consistent conditions for all variables except light.

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Bright light necessity

Bright light is specified to ensure optimal conditions for photosynthesis to occur.

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Labelled diagram purpose

The labelled diagram accurately records the experimental setup by showing the areas of light exposure and non-exposure.

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Careful foil removal

Removing the foil carefully avoids damaging the leaf tissue and allows accurate study of the covered area.

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Final iodine test aim

The test aims to reveal the presence or absence of starch in different parts of the leaf, demonstrating the effect of light.

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Blue-black color in exposed area

The expected result of a blue-black color in the light-exposed area indicates the presence of starch produced during photosynthesis.

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No color change in covered area

The expected result of no color change in the covered area indicates the absence of starch due to the lack of light.

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Comparison of iodine tests

Comparing the iodine tests before and after light exposure helps demonstrate the effect of light on starch production.

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Blue-black color in exposed area conclusion

The observation of blue-black color in the exposed area supports the conclusion that light is essential for photosynthesis.

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Successful experiment results

The results are considered successful if they show starch production only in the light-exposed area, supporting the idea that light is necessary for photosynthesis.

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Experiment in darkness outcome

Conducting the experiment in complete darkness would result in no starch production in any part of the leaf.

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Experiment duration variation

Increasing the duration of light exposure from 4 hours to 8 hours could lead to more pronounced starch presence in the light-exposed areas.

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Similar results in both areas

If both covered and uncovered parts of the leaf show the same result after the final iodine test, it indicates a potential error in the experiment.

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Red light experiment

Using red light instead of bright white light might result in less starch production, as red light is less effective for photosynthesis.

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Chlorophyll involvement

The positive results of the experiment support the idea that chlorophyll plays a role in the light-dependent reactions of photosynthesis.

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Study Notes

Aim of Experiment

  • To determine if light is necessary for photosynthesis

Apparatus and Materials

  • Boiling tube
  • Beaker (250 cm³)
  • Forceps
  • Glass rod
  • Scissors
  • Wire gauze
  • Insulating mat
  • Gas lighter
  • Potted plant with leaves
  • Aluminium foil
  • Paper clip
  • White tile
  • Bunsen burner
  • Table lamp
  • Iodine solution
  • Alcohol (70%)
  • Tripod

Procedure

  • Obtain a destarched plant and select a leaf.
  • Test the leaf for starch using iodine solution (see Practical 7.3).
  • Cover part of a leaf with aluminium foil.
  • The foil-covered part is the control (not exposed to light).
  • Draw a labeled diagram of the leaf showing the covered and uncovered parts.
  • Place the plant in bright light for 4 hours.
  • Remove the foil from the leaf.
  • Test the leaf for starch using iodine solution.

Safety Precautions

  • Wear disposable gloves.
  • Avoid contact with iodine, as it is harmful.
  • Use the minimum amount of alcohol possible and ensure the room is well-ventilated.
  • Only use alcohol in the absence of a naked flame.
  • Alcohol is flammable.

Control Set-up

  • A control set-up is used to ensure that any changes observed are due to the factor under investigation and not other factors.
  • The control setup is identical to the experimental setup except the factor under investigation is absent (no light in this case).

Variables

  • Independent Variable: Presence of light
  • Dependent Variable: Presence of starch
  • Controlled Variables: Supply of CO2, water, temperature, chlorophyll, the supply of light, and temperature.

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