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Questions and Answers
What is the purpose of wrapping a part of the leaf with aluminium foil in the experiment?
What is the purpose of wrapping a part of the leaf with aluminium foil in the experiment?
What is the function of iodine solution in the experiment?
What is the function of iodine solution in the experiment?
What is indicated by the presence of starch in the leaf after the experiment?
What is indicated by the presence of starch in the leaf after the experiment?
Why is a control set-up important in this experiment?
Why is a control set-up important in this experiment?
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What safety precautions must be taken when using alcohol in the experiment?
What safety precautions must be taken when using alcohol in the experiment?
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What is the significance of using a destarched plant in this experiment?
What is the significance of using a destarched plant in this experiment?
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What is the purpose of using a destarched plant from the beginning of this experiment?
What is the purpose of using a destarched plant from the beginning of this experiment?
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What part of the plant is used as a 'control' in this experiment?
What part of the plant is used as a 'control' in this experiment?
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In this experiment, what is the independent variable?
In this experiment, what is the independent variable?
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What are two important factors that are kept constant during this experiment?
What are two important factors that are kept constant during this experiment?
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What would you expect to observe when you test the part of the leaf that was exposed to light?
What would you expect to observe when you test the part of the leaf that was exposed to light?
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What would you expect to observe after testing the part of the leaf that was covered with aluminium foil?
What would you expect to observe after testing the part of the leaf that was covered with aluminium foil?
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What would be the expected result, if the experiment was conducted in complete darkness instead of bright light?
What would be the expected result, if the experiment was conducted in complete darkness instead of bright light?
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What would be a reasonable conclusion to draw from the experiment, if both the covered and uncovered parts of the leaf show the same results?
What would be a reasonable conclusion to draw from the experiment, if both the covered and uncovered parts of the leaf show the same results?
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If the experiment was repeated with red light, instead of bright white light, what might be the difference in the results?
If the experiment was repeated with red light, instead of bright white light, what might be the difference in the results?
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Study Notes
Aim of Experiment
- To determine if light is necessary for photosynthesis
Apparatus and Materials
- Boiling tube
- Beaker (250 cm³)
- Forceps
- Glass rod
- Scissors
- Wire gauze
- Insulating mat
- Gas lighter
- Potted plant with leaves
- Aluminium foil
- Paper clip
- White tile
- Bunsen burner
- Table lamp
- Iodine solution
- Alcohol (70%)
- Tripod
Procedure
- Obtain a destarched plant and select a leaf.
- Test the leaf for starch using iodine solution (see Practical 7.3).
- Cover part of a leaf with aluminium foil.
- The foil-covered part is the control (not exposed to light).
- Draw a labeled diagram of the leaf showing the covered and uncovered parts.
- Place the plant in bright light for 4 hours.
- Remove the foil from the leaf.
- Test the leaf for starch using iodine solution.
Safety Precautions
- Wear disposable gloves.
- Avoid contact with iodine, as it is harmful.
- Use the minimum amount of alcohol possible and ensure the room is well-ventilated.
- Only use alcohol in the absence of a naked flame.
- Alcohol is flammable.
Control Set-up
- A control set-up is used to ensure that any changes observed are due to the factor under investigation and not other factors.
- The control setup is identical to the experimental setup except the factor under investigation is absent (no light in this case).
Variables
- Independent Variable: Presence of light
- Dependent Variable: Presence of starch
- Controlled Variables: Supply of CO2, water, temperature, chlorophyll, the supply of light, and temperature.
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Description
Test your understanding of the experiment to determine if light is essential for photosynthesis. This quiz covers the apparatus used, the procedure followed, and important safety precautions. Perfect for reinforcing your knowledge of plant biology.