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Questions and Answers
What is the purpose of wrapping a part of the leaf with aluminium foil in the experiment?
What is the purpose of wrapping a part of the leaf with aluminium foil in the experiment?
Why is it important to include a control set-up in the experiment?
Why is it important to include a control set-up in the experiment?
What safety precaution should be taken when using alcohol in the experiment?
What safety precaution should be taken when using alcohol in the experiment?
Which of the following steps is taken after wrapping the leaf and exposing it to light?
Which of the following steps is taken after wrapping the leaf and exposing it to light?
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In the context of photosynthesis, what does the term 'destarched plant' refer to?
In the context of photosynthesis, what does the term 'destarched plant' refer to?
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What result is expected from the part of the leaf that is not exposed to light?
What result is expected from the part of the leaf that is not exposed to light?
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Which apparatus is NOT mentioned as necessary for conducting the experiment?
Which apparatus is NOT mentioned as necessary for conducting the experiment?
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What is the significance of performing the iodine test on the leaf?
What is the significance of performing the iodine test on the leaf?
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What is the purpose of using a destarched plant in the experiment?
What is the purpose of using a destarched plant in the experiment?
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What is the purpose of the initial iodine test?
What is the purpose of the initial iodine test?
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What is the purpose of wrapping part of the leaf with aluminium foil?
What is the purpose of wrapping part of the leaf with aluminium foil?
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What is the significance of drawing a labelled diagram of the leaf showing which parts are exposed to light and which part is not?
What is the significance of drawing a labelled diagram of the leaf showing which parts are exposed to light and which part is not?
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Why is it important to remove the aluminium foil carefully?
Why is it important to remove the aluminium foil carefully?
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What does the final iodine test aim to reveal?
What does the final iodine test aim to reveal?
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What would you expect to observe in the part of the leaf that was exposed to light, after completing Step 9?
What would you expect to observe in the part of the leaf that was exposed to light, after completing Step 9?
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What result would you anticipate for the part of the leaf that was covered with aluminum foil in Step 4?
What result would you anticipate for the part of the leaf that was covered with aluminum foil in Step 4?
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How would the results of the iodine test in Step 3 likely compare to those in Step 9?
How would the results of the iodine test in Step 3 likely compare to those in Step 9?
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What conclusion can be drawn if the exposed part of the leaf turns blue-black with iodine in Step 9?
What conclusion can be drawn if the exposed part of the leaf turns blue-black with iodine in Step 9?
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If the experiment is successful, how would you expect the results to support the aim stated at the beginning?
If the experiment is successful, how would you expect the results to support the aim stated at the beginning?
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What would be the expected result if the experiment was conducted in complete darkness instead of bright light in Step 6?
What would be the expected result if the experiment was conducted in complete darkness instead of bright light in Step 6?
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How might the results differ if the experiment was conducted over 8 hours instead of 4 hours in Step 6?
How might the results differ if the experiment was conducted over 8 hours instead of 4 hours in Step 6?
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What would be a reasonable conclusion if both covered and uncovered parts of the leaf show the same result in Step 9?
What would be a reasonable conclusion if both covered and uncovered parts of the leaf show the same result in Step 9?
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If the experiment is repeated with a red light instead of bright white light in Step 6, how might the results change?
If the experiment is repeated with a red light instead of bright white light in Step 6, how might the results change?
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What additional conclusion about chlorophyll can be drawn if the experiment shows positive results?
What additional conclusion about chlorophyll can be drawn if the experiment shows positive results?
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Study Notes
Aim
- To determine if light is needed for photosynthesis.
Apparatus and Materials
- Boiling tube (1)
- Insulating mat (2)
- Gas lighter (1)
- Beaker (250 cm³) (1)
- Tripod (1)
- Forceps (1 pair)
- Wire gauze (1)
- Scissors (1 pair)
- Glass rod (1)
- Aluminium foil (1 piece)
- Paper clip (1)
- Iodine solution (1)
- Alcohol (70%) (1)
- White tile (1)
- Bunsen burner (1)
- Table lamp (1)
- Potted plant with leaves (1)
Carrying out the Experiment
- Use a destarched plant. Choose a leaf.
- Test for starch in the leaf (see Practical 7.3).
- Cover part of a leaf with aluminum foil. This part will not be exposed to light. This is the “control”.
- Draw a diagram labeling the leaf area exposed to light and the area covered.
- Place plant in bright light for four hours.
- Carefully perform the iodine test again to test for the presence of starch in the leaf.
- Remove the aluminium foil from the leaf.
- Observe and draw a labelled diagram on which part of the leaf was exposed to light and which wasn't.
- Note: Use disposable gloves when working with chemicals. Use small amounts of flammable alcohol and use only in areas with no naked flames.
Designing Control Set-Ups
- A control setup is needed to determine if changes observed in an experiment are due to the factor of interest, or other factors. The control setup should be the same as the experimental setup, except the variable under investigation is absent.
- Examples: The experimental setup is exposed to light; the control setup is not exposed to light.
Identifying Variables
- Independent variable: Presence of light
- Dependent variable: Presence of starch
- Controlled variables: Supply of Carbon dioxide, Supply of water, Supply of oxygen, Temperature, Presence of chlorophyll, Supply of light.
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Description
Test your knowledge on the experiment to determine the necessity of light for photosynthesis. This quiz covers the materials used, the procedure, and the analysis of the results. Perfect for biology students studying plant processes and their importance.