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Questions and Answers
What is the purpose of boiling a leaf in water during the starch test?
What is the purpose of boiling a leaf in water during the starch test?
- To enhance the starch color reaction
- To kill the cells and break down cell membranes (correct)
- To soften the leaf for testing
- To remove chlorophyll from the leaf
What color change indicates the presence of starch in the leaf during the iodine test?
What color change indicates the presence of starch in the leaf during the iodine test?
- Green to yellow
- White to pink
- Yellow to purple
- Orange-brown to blue-black (correct)
In the experiment using a variegated leaf, what happens to the areas without chlorophyll when tested for starch?
In the experiment using a variegated leaf, what happens to the areas without chlorophyll when tested for starch?
- They turn blue-black
- They turn white
- They remain orange-brown (correct)
- They turn green
Why is it essential to turn off the Bunsen burner during the ethanol stage of the starch test?
Why is it essential to turn off the Bunsen burner during the ethanol stage of the starch test?
Which part of the leaf undergoes photosynthesis based on the starch test results?
Which part of the leaf undergoes photosynthesis based on the starch test results?
What factors can limit the rate of photosynthesis when the rate is constant?
What factors can limit the rate of photosynthesis when the rate is constant?
Which pathway accurately describes how carbon dioxide reaches chloroplasts in a leaf?
Which pathway accurately describes how carbon dioxide reaches chloroplasts in a leaf?
What structure in a dicotyledonous plant leaf primarily aids in gas exchange?
What structure in a dicotyledonous plant leaf primarily aids in gas exchange?
Which of the following structures primarily contains chloroplasts?
Which of the following structures primarily contains chloroplasts?
What occurs when carbon dioxide concentration in the leaf is sufficiently high?
What occurs when carbon dioxide concentration in the leaf is sufficiently high?
What is the primary function of chlorophyll in the process of photosynthesis?
What is the primary function of chlorophyll in the process of photosynthesis?
Which of the following elements is NOT required for the synthesis of carbohydrates during photosynthesis?
Which of the following elements is NOT required for the synthesis of carbohydrates during photosynthesis?
What happens to the glucose produced during photosynthesis in plants?
What happens to the glucose produced during photosynthesis in plants?
Which mineral ion is specifically needed for the formation of chlorophyll?
Which mineral ion is specifically needed for the formation of chlorophyll?
What is the result of a nitrogen deficiency in plants?
What is the result of a nitrogen deficiency in plants?
Which of these statements about photosynthesis is true?
Which of these statements about photosynthesis is true?
In what form do plants transport carbohydrates after photosynthesis?
In what form do plants transport carbohydrates after photosynthesis?
What is the primary waste product released during photosynthesis?
What is the primary waste product released during photosynthesis?
What role do root hair cells play in a plant?
What role do root hair cells play in a plant?
Which carbohydrate is converted from glucose to serve as an energy store in plants?
Which carbohydrate is converted from glucose to serve as an energy store in plants?
What is the safest method to heat ethanol in a laboratory setting?
What is the safest method to heat ethanol in a laboratory setting?
What process is investigated by covering a leaf with aluminium foil before exposing it to sunlight?
What process is investigated by covering a leaf with aluminium foil before exposing it to sunlight?
What is the purpose of destarching a plant before investigating photosynthesis?
What is the purpose of destarching a plant before investigating photosynthesis?
During gas exchange, what do plants do at night?
During gas exchange, what do plants do at night?
Which variable can limit the rate of photosynthesis when in short supply?
Which variable can limit the rate of photosynthesis when in short supply?
How can the rate of photosynthesis be accurately measured in pondweed?
How can the rate of photosynthesis be accurately measured in pondweed?
What happens to the colour of hydrogencarbonate indicator in the presence of excess carbon dioxide?
What happens to the colour of hydrogencarbonate indicator in the presence of excess carbon dioxide?
What is the effect of increased light intensity on the rate of photosynthesis?
What is the effect of increased light intensity on the rate of photosynthesis?
Which substance absorbs carbon dioxide in the experiment investigating its effect on photosynthesis?
Which substance absorbs carbon dioxide in the experiment investigating its effect on photosynthesis?
What indicates that a plant leaf conducts photosynthesis when placed in bright light?
What indicates that a plant leaf conducts photosynthesis when placed in bright light?
Why is water not considered a limiting factor in photosynthesis despite being necessary?
Why is water not considered a limiting factor in photosynthesis despite being necessary?
What type of plant is commonly used in experiments to investigate the rate of photosynthesis?
What type of plant is commonly used in experiments to investigate the rate of photosynthesis?
What conclusion can be drawn from the colour change in the hydrogencarbonate indicator when no leaf is present in light?
What conclusion can be drawn from the colour change in the hydrogencarbonate indicator when no leaf is present in light?
Flashcards
What is photosynthesis?
What is photosynthesis?
The process by which plants use sunlight, carbon dioxide, and water to produce glucose (sugar) and oxygen.
What is chlorophyll?
What is chlorophyll?
A green pigment found in chloroplasts within plant cells. It absorbs light energy, especially red and blue wavelengths.
What is the main product of photosynthesis?
What is the main product of photosynthesis?
The energy stored within glucose molecules, produced during photosynthesis.
How is glucose used by plants?
How is glucose used by plants?
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What is starch?
What is starch?
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What is cellulose?
What is cellulose?
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What is respiration?
What is respiration?
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What is sucrose?
What is sucrose?
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What is nectar?
What is nectar?
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Why are mineral ions important for plants?
Why are mineral ions important for plants?
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What is the limiting factor principle?
What is the limiting factor principle?
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How does carbon dioxide get to the chloroplast?
How does carbon dioxide get to the chloroplast?
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What is the upper epidermis?
What is the upper epidermis?
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What is the palisade mesophyll?
What is the palisade mesophyll?
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What is the spongy mesophyll?
What is the spongy mesophyll?
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Describe the process of photosynthesis.
Describe the process of photosynthesis.
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What is a controlled experiment?
What is a controlled experiment?
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What is a destarched plant?
What is a destarched plant?
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What is the rate of photosynthesis?
What is the rate of photosynthesis?
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What is a practical investigation?
What is a practical investigation?
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What is net gas exchange during the day?
What is net gas exchange during the day?
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What is net gas exchange at night?
What is net gas exchange at night?
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What is hydrogencarbonate indicator?
What is hydrogencarbonate indicator?
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What is a limiting factor?
What is a limiting factor?
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What is enzyme denaturation?
What is enzyme denaturation?
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What is the balanced chemical equation for photosynthesis?
What is the balanced chemical equation for photosynthesis?
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What are the main factors that limit the rate of photosynthesis?
What are the main factors that limit the rate of photosynthesis?
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Starch Test
Starch Test
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Variegated Leaf
Variegated Leaf
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Decolorizing Chlorophyll
Decolorizing Chlorophyll
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Photosynthetic Area
Photosynthetic Area
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Iodine Staining
Iodine Staining
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Study Notes
Photosynthesis
- Green plants produce glucose using carbon dioxide and water, releasing oxygen as a byproduct.
- The process requires energy, captured by chlorophyll, a green pigment in chloroplasts.
- Photosynthesis's chemical equation is: 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂
Chlorophyll
- Chlorophyll is a green pigment located in chloroplasts within plant cells.
- It absorbs light energy, converting it to chemical energy for glucose synthesis.
- Photosynthesis cannot occur without chlorophyll.
Use & Storage of Carbohydrates
- Glucose, produced during photosynthesis, can be converted into starch as an energy storage form.
- Cellulose forms plant cell walls.
- Glucose turns into sucrose for transport in phloem.
- Sucrose is converted into fats and oils for storage in seeds.
- Carbohydrates are combined with nutrients from the soil (nitrogen and other minerals) to create amino acids, which in turn form proteins.
Minerals in Plants
- Plants need minerals beside carbohydrates to create proteins, lipids and DNA.
- Plants require nitrogen (in nitrates) for amino acids.
- Magnesium is a component of chlorophyll.
- Mineral deficiencies can cause leaf yellowing (chlorosis) and stunted growth.
Investigating the Need for Chlorophyll, Light & Carbon Dioxide
- Starch is a reliable indicator for photosynthesis.
- Leaves can be tested for starch using iodine (turns blue-black if present).
- A leaf must be destarched before testing.
- Lack of light or carbon dioxide prevents starch formation.
Investigating the Need for Light
- Plants must be destarched prior to an experiment to ensure there is no pre-existing starch.
- Covering a leaf partially with foil before exposing it to light, shows areas that receive light create starch. Areas that don't produce starch remain orange-brown.
Investigating the Need for Carbon Dioxide
- Plants needing carbon dioxide are tested using sodium hydroxide to take up the CO2, preventing photosynthesis.
- Leaves are tested for starch following experiment to see if photosynthesis happened.
Investigating the Rate of Photosynthesis
- Elodea or Cabomba are commonly used in experiments that measure the rate of photosynthesis by counting the oxygen bubbles produced over time.
- Measuring oxygen volume or using hydrogen carbonate (a pH indicator) can provide a more precise measure.
Investigating Gas Exchange
- Plants constantly respire, consuming oxygen and producing carbon dioxide.
- Photosynthesis takes in carbon dioxide and releases oxygen.
- Hydrogen carbonate indicator changes color depending on pH, which indicates gas exchange fluctuations. Higher CO2 causes lower pH; lower CO2 causes a higher pH.
Photosynthesis Chemical Equation
- Photosynthesis's balanced equation is: 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂ (with energy from light).
Limiting Factors
- Factors like temperature, light intensity, and carbon dioxide concentration can limit photosynthesis.
- Optimal temperatures for enzymes maximize reaction rates.
- Increasing light intensity generally increases the rate up to a certain point.
- The rate of photosynthesis plateaus when another factor becomes limiting.
Leaf Structure
- Leaves have a large surface area for maximizing light absorption and gas exchange.
- Thin cuticle reduces water loss.
- Chlorophyll in palisade mesophyll cells captures light efficiently for photosynthesis.
- Spongy mesophyll air spaces increase gas diffusion.
- Stomata allow CO2 intake and oxygen release.
Identifying Leaf Structures
- Key structures in a dicotyledonous leaf include upper and lower epidermis, palisade mesophyll, spongy mesophyll, stomata, guard cells, and vascular bundles.
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