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Pharmacology Problems: Calculating Drug Dosage and Euthanasia

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Pharmacology Problems: Calculating Drug Dosage and Euthanasia

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Questions and Answers

In which type of experiment are isolated organs or tissues used?

Ex vivo

In vitro (correct)

In situ

In vivo

What is an advantage of working in vitro?

Easy to adjust experimental factors

Avoid interference by hormones and neurotransmitters (correct)

Larger doses of the drug are required

Biotransformation, distribution, and elimination are accounted for

What is a crucial factor to consider when working in vitro?

Type of animal used

Color of the equipment

pH of the solution (correct)

Brand of the experimental instruments

How should rabbits be handled during experimentation?

With minimal pressure or excitement Signup and view all the answers

What is the route of administration for a drug in frogs?

Subcutaneous (SC) Signup and view all the answers

What is the formula to calculate the drug volume to be administered?

Weight x Suggested drug dose / Drug concentration Signup and view all the answers

What is the concentration of the drug required in this case?

2% (2g / 100ml) Signup and view all the answers

What is the purpose of euthanizing animals at the end of a study?

For the purpose of sample collection or postmortem examination Signup and view all the answers

What is the recommended method of euthanasia for rodents?

Carbon dioxide (CO2) or Sodium Pentobarbital Signup and view all the answers

What is the site of blood collection for mice?

Cardiac, orbital sinus, tail vein, facial vein Signup and view all the answers

What is the minimum dose of Sodium Pentobarbital required for euthanasia in rodents?

100 mg/kg IV, IP Signup and view all the answers

Why is anesthesia required for blood collection in animals?

To reduce the animal's stress and pain Signup and view all the answers