Pharmacology Lab: Bioassay and Biosafety Unit 2
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Questions and Answers

Which type of bioassay uses microorganisms to determine the antimicrobial potency of antibiotics?

  • Graded assays
  • Four point Bioassay
  • Antimicrobial bioassay (correct)
  • Disk diffusion assay
  • What is the gold standard assay mentioned in the text for determining antimicrobial potency?

  • MBC
  • MBSC
  • MIC
  • Disk diffusion assay (correct)
  • Which method is used in the Antifungal bioassay that involves the use of fungal spores?

  • Paper disc method (correct)
  • Four point Bioassay
  • Antimitotic assay
  • Cylinder plate method
  • What are examples of assays that involve determining concentrations like MIC, MBC, and MBSC?

    <p>Minimum Inhibitory Concentration</p> Signup and view all the answers

    Which type of assay evaluates cytokine release and cell maturation using cells from whole bone marrow?

    <p>Antimicrobial assay</p> Signup and view all the answers

    What type of agents are involved in antimitotic assays based on their impact on the mitotic cycle?

    <p>Pharmacological agents</p> Signup and view all the answers

    What is the main purpose of a bioassay in pharmacology?

    <p>To estimate the concentration of a substance in a biological system</p> Signup and view all the answers

    Which term best describes a bioassay that involves applying a specific substance to a biological organism to observe physical, biological, and chemical changes?

    <p>Indirect Assay</p> Signup and view all the answers

    What is the key difference between Quantal and Graded assays in bioassays?

    <p>Graded assays measure responses in categories while Quantal assays measure continuous responses</p> Signup and view all the answers

    In indirect bioassays, which method involves determining the concentration of a substance by comparing its effects with those of standard concentrations?

    <p>Interpolation</p> Signup and view all the answers

    Which of the following is an example of an Existing and Emerging Bioassay as mentioned in the text?

    <p>Reporter-based biochemical bioassays</p> Signup and view all the answers

    What type of bioassay would involve using biosensors to detect binding interactions between biological molecules?

    <p>Biosensor-based binding bioassays</p> Signup and view all the answers

    What is the typical subject in a bioassay?

    <p>An animal or tissue</p> Signup and view all the answers

    Which of the following is not a common agent in a bioassay?

    <p>Geological agent</p> Signup and view all the answers

    In a bioassay, what is the purpose of the test substance?

    <p>To observe the response of the subject</p> Signup and view all the answers

    What types of responses are common in bioassays?

    <p>Binary, continuous, or characteristic responses</p> Signup and view all the answers

    In the principle of bioassay, why is it important for the active agent to show a similar response in different animal species?

    <p>To ensure consistent response measurement</p> Signup and view all the answers

    What distinguishes quantitative assays from qualitative assays?

    <p>Quantitative assays provide numerical analysis, while qualitative assays do not.</p> Signup and view all the answers

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