Pharmacology Lab: Bioassay and Biosafety Unit 2
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Questions and Answers

Which type of bioassay uses microorganisms to determine the antimicrobial potency of antibiotics?

  • Graded assays
  • Four point Bioassay
  • Antimicrobial bioassay (correct)
  • Disk diffusion assay

What is the gold standard assay mentioned in the text for determining antimicrobial potency?

  • MBC
  • MBSC
  • MIC
  • Disk diffusion assay (correct)

Which method is used in the Antifungal bioassay that involves the use of fungal spores?

  • Paper disc method (correct)
  • Four point Bioassay
  • Antimitotic assay
  • Cylinder plate method

What are examples of assays that involve determining concentrations like MIC, MBC, and MBSC?

<p>Minimum Inhibitory Concentration (D)</p> Signup and view all the answers

Which type of assay evaluates cytokine release and cell maturation using cells from whole bone marrow?

<p>Antimicrobial assay (D)</p> Signup and view all the answers

What type of agents are involved in antimitotic assays based on their impact on the mitotic cycle?

<p>Pharmacological agents (D)</p> Signup and view all the answers

What is the main purpose of a bioassay in pharmacology?

<p>To estimate the concentration of a substance in a biological system (D)</p> Signup and view all the answers

Which term best describes a bioassay that involves applying a specific substance to a biological organism to observe physical, biological, and chemical changes?

<p>Indirect Assay (D)</p> Signup and view all the answers

What is the key difference between Quantal and Graded assays in bioassays?

<p>Graded assays measure responses in categories while Quantal assays measure continuous responses (C)</p> Signup and view all the answers

In indirect bioassays, which method involves determining the concentration of a substance by comparing its effects with those of standard concentrations?

<p>Interpolation (C)</p> Signup and view all the answers

Which of the following is an example of an Existing and Emerging Bioassay as mentioned in the text?

<p>Reporter-based biochemical bioassays (A)</p> Signup and view all the answers

What type of bioassay would involve using biosensors to detect binding interactions between biological molecules?

<p>Biosensor-based binding bioassays (D)</p> Signup and view all the answers

What is the typical subject in a bioassay?

<p>An animal or tissue (A)</p> Signup and view all the answers

Which of the following is not a common agent in a bioassay?

<p>Geological agent (A)</p> Signup and view all the answers

In a bioassay, what is the purpose of the test substance?

<p>To observe the response of the subject (A)</p> Signup and view all the answers

What types of responses are common in bioassays?

<p>Binary, continuous, or characteristic responses (B)</p> Signup and view all the answers

In the principle of bioassay, why is it important for the active agent to show a similar response in different animal species?

<p>To ensure consistent response measurement (B)</p> Signup and view all the answers

What distinguishes quantitative assays from qualitative assays?

<p>Quantitative assays provide numerical analysis, while qualitative assays do not. (B)</p> Signup and view all the answers

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