pET Expression Vector Components

The ______ gene provides resistance to the antibiotic ampicillin.

The ______ terminator signals the end of transcription for T7 RNA polymerase.

The ______ ori is the origin of replication from the pBR322 plasmid.

The Rop gene codes for proteins involved in the ______ of the plasmid.

The LacI protein represses transcription of the gene of interest from the ______ promoter.

E. coli is particularly popular when the aim of the cloning experiment is to study the basic features of ______ biology.

Cloning vectors for ______ are used when the aim of the experiment is to study gene structure and function.

The pET expression vector is approximately ______ KB in size.

In Gateway cloning, a ______ is moved into a vector through a two-step recombination process.

Lambda phage utilizes recombination machinery to integrate its genome into the host bacterial ______ during the lysogenic cycle.

The integration reactions in Gateway cloning are mediated by a site-specific ______ system derived from bacteriophage lambda.

The recombination of attP and attB sites creates ______ and attR sites.

The Gateway system involves two key ______ sites: attB and attP.

The BP reaction is involved in the creation of ______ clones.

The LR reaction takes place between the attL sites of the generated ______ clone and the attR sites of the destination vector.

Gateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired ______ sequences.

Hydrogen bonding and ______ interactions occur between proteins and DNA.

Open chromatin regions have ______ accessibility to transcription initiation complex.

The stable promotor sequence is characterized by a ______ rich sequence.

Transcription factors bind to specific ______ in the DNA known as a consensus site.

The rate of peptide chain elongation increases with respect to an increase in ______.

Strong promoters have high affinity for ______ polymerase, leading to efficient recruitment and initiation of transcription.

Epigenetic modifications include ______ methylation and histone acetylation.

At higher ______, the rate of cHSPA6 synthesis was faster due to an increase in plasmid copy number, transcription rate, and peptide chain elongation rate.

Every protein folds differently depending upon its characteristics such as ______, hydrophobicity, post-translational modifications, isoelectric points, etc.

Thermus thermophilus, an extremophile, has a ______ rich genome to tolerate high environmental temperatures.

The presence and affinity of transcription factor ______ sites influence the recruitment of RNA polymerase and the efficiency of transcription initiation.

The final growth varied due to different ______ rates under each media.

TB medium showed the highest ______ rate under uninduced conditions.

The induced culture in the TB medium expressing cHSPA6 showed the lowest ______ yield.

The LB media showed relatively higher ______ rate under induced culture conditions resulting in higher volumetric yield.

If the rate of protein synthesis supersedes ______ machinery, the recombinant protein will not fold correctly.

Promoters may contain additional regulatory elements such as ______ and silencers, which modulate gene expression in response to cellular signals or environmental cues.

The presence and activity of these elements can influence ______ strength by affecting the rate of transcription initiation.

Consult published literature and databases to identify ______ with proven performance in specific host systems.

Experiment with different ______ to determine their suitability for driving protein expression in your specific application.

Use bioinformatics tools to predict ______ strength, specificity, and other relevant properties based on sequence analysis.

Conduct a comprehensive review of published literature and databases to identify ______ with documented performance in specific host systems or for similar protein expression applications.

Evaluate protein expression levels using quantitative assays such as Western blotting or ______ microscopy.

Utilize resources such as PubMed, ______ databases and scientific journals to gather relevant information.