Podcast
Questions and Answers
Which type of DNA is used as cloning vectors in plant molecular biology?
Which type of DNA is used as cloning vectors in plant molecular biology?
- Linear DNA
- Circular DNA (correct)
- Single-stranded DNA
- Double-stranded DNA
What advantage does the small size of cloning vectors provide?
What advantage does the small size of cloning vectors provide?
- Increased stability
- Easy identification (correct)
- Enhanced replication
- Flexibility in gene transfer
What is a desirable feature of plasmid vectors used in plant molecular biology?
What is a desirable feature of plasmid vectors used in plant molecular biology?
- Inability to replicate independently
- Ability to confer selectable phenotypic traits (correct)
- Multiple sites for restriction enzymes
- Ability to induce crown gall disease
When were cloning vectors for higher plants developed?
When were cloning vectors for higher plants developed?
What is the Ti plasmid of Agrobacterium tumefaciens known for?
What is the Ti plasmid of Agrobacterium tumefaciens known for?
Study Notes
Cloning Vectors in Plant Molecular Biology
- Binary vectors and Agrobacterium tumefaciens are used as cloning vectors in plant molecular biology.
Advantages of Cloning Vectors
- The small size of cloning vectors provides an advantage as it allows for easier manipulation and high transformation efficiency.
Plasmid Vectors
- A desirable feature of plasmid vectors used in plant molecular biology is that they are high-copy number plasmids.
Development of Cloning Vectors
- Cloning vectors for higher plants were developed in the 1980s.
Ti Plasmid of Agrobacterium tumefaciens
- The Ti plasmid of Agrobacterium tumefaciens is known for its ability to transfer DNA to plant cells, causing crown gall disease.
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Description
Test your knowledge on vectors used in plant molecular biology and plasmids. Discover why these vectors are suitable for cloning, including their closed circular DNA structure, small size, ability to maintain stable copy number, independent replication in host cells, and passing on information to daughter cells. Explore additional desirable features of plasmid vectors such as conferring selectable phenotypic traits on host cells.
