Vectors and Plasmids in Plant Molecular Biology
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Questions and Answers

Which type of DNA is used as cloning vectors in plant molecular biology?

  • Linear DNA
  • Circular DNA (correct)
  • Single-stranded DNA
  • Double-stranded DNA
  • What advantage does the small size of cloning vectors provide?

  • Increased stability
  • Easy identification (correct)
  • Enhanced replication
  • Flexibility in gene transfer
  • What is a desirable feature of plasmid vectors used in plant molecular biology?

  • Inability to replicate independently
  • Ability to confer selectable phenotypic traits (correct)
  • Multiple sites for restriction enzymes
  • Ability to induce crown gall disease
  • When were cloning vectors for higher plants developed?

    <p>1980s</p> Signup and view all the answers

    What is the Ti plasmid of Agrobacterium tumefaciens known for?

    <p>Causing crown gall disease</p> Signup and view all the answers

    Study Notes

    Cloning Vectors in Plant Molecular Biology

    • Binary vectors and Agrobacterium tumefaciens are used as cloning vectors in plant molecular biology.

    Advantages of Cloning Vectors

    • The small size of cloning vectors provides an advantage as it allows for easier manipulation and high transformation efficiency.

    Plasmid Vectors

    • A desirable feature of plasmid vectors used in plant molecular biology is that they are high-copy number plasmids.

    Development of Cloning Vectors

    • Cloning vectors for higher plants were developed in the 1980s.

    Ti Plasmid of Agrobacterium tumefaciens

    • The Ti plasmid of Agrobacterium tumefaciens is known for its ability to transfer DNA to plant cells, causing crown gall disease.

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    Description

    Test your knowledge on vectors used in plant molecular biology and plasmids. Discover why these vectors are suitable for cloning, including their closed circular DNA structure, small size, ability to maintain stable copy number, independent replication in host cells, and passing on information to daughter cells. Explore additional desirable features of plasmid vectors such as conferring selectable phenotypic traits on host cells.

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