Podcast
Questions and Answers
What is the optimal temperature for the DNA polymerase enzyme to synthesize new DNA strands during the extension step of PCR?
What is the optimal temperature for the DNA polymerase enzyme to synthesize new DNA strands during the extension step of PCR?
- 72°C (correct)
- 95°C
- 85°C
- 65°C
What is the primary function of primers in PCR?
What is the primary function of primers in PCR?
- To provide a starting point for DNA synthesis (correct)
- To break down glucose for energy and carbon molecules
- To provide energy for DNA synthesis
- To catalyze the sixth step of glycolysis
What is the typical length range of PCR primers?
What is the typical length range of PCR primers?
- 50 to 60 nucleotides
- 10 to 15 nucleotides
- 30 to 40 nucleotides
- 18 to 25 nucleotides (correct)
What is the significance of primer specificity in PCR?
What is the significance of primer specificity in PCR?
What is the name of the enzyme that catalyzes the sixth step of glycolysis and is being targeted for amplification in this experiment?
What is the name of the enzyme that catalyzes the sixth step of glycolysis and is being targeted for amplification in this experiment?
What is the primary purpose of the denaturation step in PCR?
What is the primary purpose of the denaturation step in PCR?
What is the optimal temperature range for the annealing step in PCR?
What is the optimal temperature range for the annealing step in PCR?
What is the primary characteristic of Taq polymerase that makes it suitable for PCR?
What is the primary characteristic of Taq polymerase that makes it suitable for PCR?
What is the limitation of PCR in terms of DNA fragment length?
What is the limitation of PCR in terms of DNA fragment length?
What is the ideal GC content range for PCR primers?
What is the ideal GC content range for PCR primers?
What is the role of dNTPs in DNA synthesis during PCR?
What is the role of dNTPs in DNA synthesis during PCR?
What is the consequence of significant self-complementarity in PCR primers?
What is the consequence of significant self-complementarity in PCR primers?
Which of the following is an application of PCR in genetic engineering?
Which of the following is an application of PCR in genetic engineering?
What is the role of deoxyribonucleotide triphosphates (dNTPs) in PCR?
What is the role of deoxyribonucleotide triphosphates (dNTPs) in PCR?
What is the purpose of optimizing the melting temperature (Tm) of PCR primers?
What is the purpose of optimizing the melting temperature (Tm) of PCR primers?
What is the characteristic of Taq polymerase that allows it to withstand repeated heating cycles during PCR?
What is the characteristic of Taq polymerase that allows it to withstand repeated heating cycles during PCR?
What is the primary advantage of Taq polymerase's high error rate in certain applications?
What is the primary advantage of Taq polymerase's high error rate in certain applications?
What is the typical length range limitation of DNA fragments that can be amplified using Taq polymerase?
What is the typical length range limitation of DNA fragments that can be amplified using Taq polymerase?
What is the direction in which Taq polymerase adds nucleotides to the growing DNA strand during PCR?
What is the direction in which Taq polymerase adds nucleotides to the growing DNA strand during PCR?
Why is Taq polymerase widely used in molecular biology laboratories?
Why is Taq polymerase widely used in molecular biology laboratories?
