PCR Mastery
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Questions and Answers

What is the function of primers in the PCR process?

  • Prevent DNA denaturation during PCR
  • Help in separating DNA strands
  • Act as enzymes to speed up DNA replication
  • Provide a sequence-specific initiation site for DNA polymerase (correct)

    • What is the main purpose of Polymerase Chain Reaction (PCR) in molecular biology?

  • Detect genetic mutations in DNA samples
  • Amplify specific DNA fragments into millions of copies in a few hours (correct)
  • Create RNA copies from DNA templates
  • Separate DNA fragments based on size

    • Which of the following is NOT a requirement for Conventional PCR in molecular biology?

  • Taq polymerase
  • Pairs of primers
  • Deoxy nucleotides triphosphate (dNTPs)
  • RNA template (correct)

    • In the context of molecular biology, what is the role of Deoxy nucleotides triphosphate (dNTPs) in PCR?

    <p>Building blocks for DNA polymerase to synthesize a new DNA strand</p> Signup and view all the answers

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