Mastering PCR
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Questions and Answers

What is the role of denaturation in the PCR cycle?

  • To amplify the DNA
  • To split the double strand of DNA (correct)
  • To heat up the sample for complementary base pairing
  • To cool down the sample for primer activity
  • What is the role of annealing in the PCR cycle?

  • To cool down the sample for primer activity (correct)
  • To split the double strand of DNA
  • To amplify the DNA
  • To heat up the sample for complementary base pairing
  • What is the role of extension in the PCR cycle?

  • To cool down the sample for primer activity
  • To amplify the DNA (correct)
  • To split the double strand of DNA
  • To heat up the sample for complementary base pairing
  • What is the formula for the amplification factor in the PCR cycle?

    <p>N = NO(2)^x</p> Signup and view all the answers

    What does N represent in the PCR cycle?

    <p>Product concentration</p> Signup and view all the answers

    What is the purpose of selecting the annealing temperature based on primer size?

    <p>To prevent the primers from binding to unintended locations on the DNA template</p> Signup and view all the answers

    What temperature is used for the denaturation step in the PCR cycle?

    <p>94 degrees celsius</p> Signup and view all the answers

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