Mastering PCR
7 Questions
0 Views

Mastering PCR

Created by
@CourteousEpiphany

Podcast Beta

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the role of denaturation in the PCR cycle?

  • To amplify the DNA
  • To split the double strand of DNA (correct)
  • To heat up the sample for complementary base pairing
  • To cool down the sample for primer activity
  • What is the role of annealing in the PCR cycle?

  • To cool down the sample for primer activity (correct)
  • To split the double strand of DNA
  • To amplify the DNA
  • To heat up the sample for complementary base pairing
  • What is the role of extension in the PCR cycle?

  • To cool down the sample for primer activity
  • To amplify the DNA (correct)
  • To split the double strand of DNA
  • To heat up the sample for complementary base pairing
  • What is the formula for the amplification factor in the PCR cycle?

    <p>N = NO(2)^x</p> Signup and view all the answers

    What does N represent in the PCR cycle?

    <p>Product concentration</p> Signup and view all the answers

    What is the purpose of selecting the annealing temperature based on primer size?

    <p>To prevent the primers from binding to unintended locations on the DNA template</p> Signup and view all the answers

    What temperature is used for the denaturation step in the PCR cycle?

    <p>94 degrees celsius</p> Signup and view all the answers

    More Like This

    Mastering Primer Design
    5 questions

    Mastering Primer Design

    CourteousEpiphany avatar
    CourteousEpiphany
    Mastering Options Greeks
    24 questions
    Mastering Biology Chapter 7 Flashcards
    70 questions
    Use Quizgecko on...
    Browser
    Browser