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Questions and Answers
What is the role of denaturation in the PCR cycle?
What is the role of denaturation in the PCR cycle?
- To amplify the DNA
- To split the double strand of DNA (correct)
- To heat up the sample for complementary base pairing
- To cool down the sample for primer activity
What is the role of annealing in the PCR cycle?
What is the role of annealing in the PCR cycle?
- To cool down the sample for primer activity (correct)
- To split the double strand of DNA
- To amplify the DNA
- To heat up the sample for complementary base pairing
What is the role of extension in the PCR cycle?
What is the role of extension in the PCR cycle?
- To cool down the sample for primer activity
- To amplify the DNA (correct)
- To split the double strand of DNA
- To heat up the sample for complementary base pairing
What is the formula for the amplification factor in the PCR cycle?
What is the formula for the amplification factor in the PCR cycle?
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What does N represent in the PCR cycle?
What does N represent in the PCR cycle?
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What is the purpose of selecting the annealing temperature based on primer size?
What is the purpose of selecting the annealing temperature based on primer size?
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What temperature is used for the denaturation step in the PCR cycle?
What temperature is used for the denaturation step in the PCR cycle?
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