10 Questions
What is the function of primers in PCR?
Define the region to be amplified
Which enzyme is used in PCR and why?
DNA polymerase, because it can withstand heat
What is the role of dNTP in PCR?
Provide substrate for DNA synthesis
Why was PCR not feasible before the discovery of Taq DNA polymerase?
The polymerase could not withstand the high temperatures required
What is the function of Taq DNA polymerase in PCR?
Withstand the high temperatures required
What is the function of gel electrophoresis in PCR?
To visualize the amplified DNA
What is the function of the 3'-OH end of the primer?
To initiate synthesis
What is the function of substrate dNTP in PCR?
To provide substrate for DNA synthesis
What is the source of Taq DNA polymerase in PCR?
Bacteria
What is the advantage of using Taq DNA polymerase in PCR?
It is more stable
Test your knowledge on the essential requirements needed for Polymerase Chain Reaction (PCR) with this quiz. From template DNA to primers and substrates, learn about the key components needed for successful amplification of specific DNA sequences. Sharpen your skills in defining the region to be amplified and understanding the role of gel electrophoresis in detecting duplicated amounts. Put your PCR knowledge to the test and take this quiz now!
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