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Master the Essentials of PCR

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Questions and Answers

What is the function of primers in PCR?

Define the region to be amplified (correct)

Break down the template DNA

Provide substrate for DNA synthesis

Separate the strands of template DNA

Which enzyme is used in PCR and why?

DNA polymerase, because it can withstand heat (correct)

DNA polymerase, because it is cheaper

RNA polymerase, because it is more efficient

RNA polymerase, because it is more stable

What is the role of dNTP in PCR?

Break down the template DNA

Define the region to be amplified

Provide substrate for DNA synthesis (correct)

Separate the strands of template DNA

Why was PCR not feasible before the discovery of Taq DNA polymerase?

The polymerase could not withstand the high temperatures required Signup and view all the answers

What is the function of Taq DNA polymerase in PCR?

Withstand the high temperatures required Signup and view all the answers

What is the function of gel electrophoresis in PCR?

To visualize the amplified DNA Signup and view all the answers

What is the function of the 3'-OH end of the primer?

To initiate synthesis Signup and view all the answers

What is the function of substrate dNTP in PCR?

To provide substrate for DNA synthesis Signup and view all the answers

What is the source of Taq DNA polymerase in PCR?

Bacteria Signup and view all the answers

What is the advantage of using Taq DNA polymerase in PCR?

It is more stable Signup and view all the answers

Master the Essentials of PCR

Choose a study mode

Podcast

Questions and Answers

What is the function of primers in PCR?

Which enzyme is used in PCR and why?

What is the role of dNTP in PCR?

Why was PCR not feasible before the discovery of Taq DNA polymerase?

What is the function of Taq DNA polymerase in PCR?

What is the function of gel electrophoresis in PCR?

What is the function of the 3'-OH end of the primer?

What is the function of substrate dNTP in PCR?

What is the source of Taq DNA polymerase in PCR?

What is the advantage of using Taq DNA polymerase in PCR?

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