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Questions and Answers
What is the function of primers in PCR?
What is the function of primers in PCR?
- Define the region to be amplified (correct)
- Break down the template DNA
- Provide substrate for DNA synthesis
- Separate the strands of template DNA
Which enzyme is used in PCR and why?
Which enzyme is used in PCR and why?
- DNA polymerase, because it can withstand heat (correct)
- DNA polymerase, because it is cheaper
- RNA polymerase, because it is more efficient
- RNA polymerase, because it is more stable
What is the role of dNTP in PCR?
What is the role of dNTP in PCR?
- Break down the template DNA
- Define the region to be amplified
- Provide substrate for DNA synthesis (correct)
- Separate the strands of template DNA
Why was PCR not feasible before the discovery of Taq DNA polymerase?
Why was PCR not feasible before the discovery of Taq DNA polymerase?
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What is the function of Taq DNA polymerase in PCR?
What is the function of Taq DNA polymerase in PCR?
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What is the function of gel electrophoresis in PCR?
What is the function of gel electrophoresis in PCR?
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What is the function of the 3'-OH end of the primer?
What is the function of the 3'-OH end of the primer?
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What is the function of substrate dNTP in PCR?
What is the function of substrate dNTP in PCR?
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What is the source of Taq DNA polymerase in PCR?
What is the source of Taq DNA polymerase in PCR?
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What is the advantage of using Taq DNA polymerase in PCR?
What is the advantage of using Taq DNA polymerase in PCR?
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