10 Questions
What is the optimum length range for typical primers?
18-22 bases
What is the recommended GC content for typical primers?
50%
What does the Tm of a primer pair indicate?
Temperature required to break the hydrogen bonds
What is the purpose of the denaturation step in PCR?
Separate the double-stranded template DNA
In standard PCR, what is required to visualize the DNA after amplification?
Ethidium bromide and a UV light source
What is the main purpose of Polymerase Chain Reaction (PCR)?
To create billions of copies of a specific DNA sequence
Which enzyme is commonly used in most types of PCR?
Taq polymerase
What is the role of synthetic oligonucleotide primers in PCR?
To amplify DNA fragments
What is the function of the thermal cycler (PCR machine) in PCR?
To regulate the temperature for DNA amplification
What must be included in a PCR reaction mixture?
Thermostable Taq polymerase, synthetic oligonucleotide primers, and DNA template
Test your knowledge of Polymerase Chain Reaction (PCR) in this practical session quiz. Learn about the invention of PCR and its use in exponential amplification of DNA.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
