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PCR in Biological Sciences Practical Session

10 Questions

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PCR in Biological Sciences Practical Session

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Questions and Answers

What is the optimum length range for typical primers?

28-32 bases

25-30 bases

10-15 bases

18-22 bases (correct)

What is the recommended GC content for typical primers?

40%

60%

30%

50% (correct)

What does the Tm of a primer pair indicate?

Temperature required to break the hydrogen bonds (correct)

Amount of DNA to be replicated

Number of primer pairs needed for PCR

Duration of the PCR process

What is the purpose of the denaturation step in PCR?

Separate the double-stranded template DNA Signup and view all the answers

In standard PCR, what is required to visualize the DNA after amplification?

Ethidium bromide and a UV light source Signup and view all the answers

What is the main purpose of Polymerase Chain Reaction (PCR)?

To create billions of copies of a specific DNA sequence Signup and view all the answers

Which enzyme is commonly used in most types of PCR?

Taq polymerase Signup and view all the answers

What is the role of synthetic oligonucleotide primers in PCR?

To amplify DNA fragments Signup and view all the answers

What is the function of the thermal cycler (PCR machine) in PCR?

To regulate the temperature for DNA amplification Signup and view all the answers

What must be included in a PCR reaction mixture?

Thermostable Taq polymerase, synthetic oligonucleotide primers, and DNA template Signup and view all the answers