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Questions and Answers
What is the optimum length range for typical primers?
What is the optimum length range for typical primers?
- 28-32 bases
- 25-30 bases
- 10-15 bases
- 18-22 bases (correct)
What is the recommended GC content for typical primers?
What is the recommended GC content for typical primers?
- 40%
- 60%
- 30%
- 50% (correct)
What does the Tm of a primer pair indicate?
What does the Tm of a primer pair indicate?
- Temperature required to break the hydrogen bonds (correct)
- Amount of DNA to be replicated
- Number of primer pairs needed for PCR
- Duration of the PCR process
What is the purpose of the denaturation step in PCR?
What is the purpose of the denaturation step in PCR?
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In standard PCR, what is required to visualize the DNA after amplification?
In standard PCR, what is required to visualize the DNA after amplification?
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What is the main purpose of Polymerase Chain Reaction (PCR)?
What is the main purpose of Polymerase Chain Reaction (PCR)?
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Which enzyme is commonly used in most types of PCR?
Which enzyme is commonly used in most types of PCR?
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What is the role of synthetic oligonucleotide primers in PCR?
What is the role of synthetic oligonucleotide primers in PCR?
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What is the function of the thermal cycler (PCR machine) in PCR?
What is the function of the thermal cycler (PCR machine) in PCR?
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What must be included in a PCR reaction mixture?
What must be included in a PCR reaction mixture?
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