30 Questions
Which step comes after Fixation in the routine tissue processing?
Dehydration
What is the aim of the Paraffin Technique in routine tissue processing?
To prepare tissues in a supporting medium ready to be cut into thin sections
What is the first step in the steps from specimen to report?
Fixation
In tissue processing, what follows Impregnation?
Tissue embedding
What is the last step in routine tissue processing?
Block & Slide archiving
What instrument is used for cutting FFPE blocks?
Microtome
What is the embedding medium of choice for routine specimens?
Paraffin wax
What is the purpose of the clearing reagent in tissue processing?
To act as a link between the dehydrator and wax
Which reagent is used in routine practice as a dehydrating solution?
Isopropyl alcohol
What is the main drawback of industrial methylated spirit (IMS)?
It is harmful by inhalation and if swallowed
What should be done if Xylene waste is produced?
Dispose of it as specialist waste
How are tissue processing schedules validated?
By using surplus clinical material not required for diagnosis
What is the role of the intermediary steps in paraffin processing?
To transfer tissue through different chemical solutions
What are the characteristics of paraffin wax that make it suitable for tissue processing?
It has a consistency very similar to that of the tissue itself and it can support the cutting of thin sections
What is the most likely result of insufficient tissue fixation and formalin penetration?
Incomplete processing affecting overall morphology, chromatin detail, tissue integrity
What is the typical color of formalin pigment seen in a formalin-fixed paraffin section of kidney?
Brown to black
What can be used to replace xylene in the processing of specimens?
Isopropyl alcohol in combination with ethanol
What is required for processing fatty tissues?
Extended time and increased temperature
What has improved, allowing for an increase in the number of larger tissue blocks processed to meet reporting demands?
Improved processing equipment
What is the embedding medium typically used for routine specimens in tissue processing?
Paraffin wax
What is the purpose of hard tissue pre-treatment in tissue embedding process?
To de-calcify the tissue
What is the aim of using a cold plate in the tissue embedding process?
To aid in tissue orientation in the mould
Which automated stainer and coverslipper is mentioned in the text?
Sakura Automated Stainer and coverslipper
What is the purpose of block checking in the quality assurance process?
To record case number and number of blocks before processing
What comes after fixation in the steps from specimen to report?
Tissue processing
What is the typical thickness of routine sections in microtomy?
3-4 microns
What is the main drawback of industrial methylated spirit (IMS) mentioned in the text?
Strong odor
What is the role of intermediary steps in paraffin processing according to the text?
To maintain tissue shape during processing
What instrument is used for cutting FFPE blocks according to the text?
Manual Sakura
What is done if Xylene waste is produced according to the text?
Treat it as hazardous waste
Learn about tissue processing, embedding, microtomy, staining, and quality assurance in the field of pathological sciences. Explore the principles, practices, and reagents used in tissue processing, as well as the techniques for embedding and cutting blocks using a microtome.
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