Parasitology Specimen Collection Guidelines

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What is the recommended number of specimens for detecting protozoa and helminths in stool?

3 specimens (correct)

2 specimens

4 specimens

1 specimen

What is the proper fixative to use for preserving protozoan cysts?

Barium

Formalin (correct)

Mineral oil

Bismuth

Why should specimens be fixed in preservative for at least 30 minutes before processing?

To reduce the shelf-life of the specimen

To enhance the color of the specimen

To preserve protozoa morphology (correct)

To halt helminth egg development

Which substance is listed as interfering with stool specimen testing?

Mineral oil Signup and view all the answers

When should stool samples be collected for testing?

Before therapy or 5 to 7 days after therapy Signup and view all the answers

What are the advantages of formalin as a fixative?

Easy to prepare and preserves specimens for several years Signup and view all the answers

Which technique is used for detecting Enterobius vermicularis eggs?

Cellophane Tape Preparation Signup and view all the answers

What is the recommended staining method for blood smears prepared from fresh whole blood without anticoagulant?

Wright’s stain Signup and view all the answers

What is the recommended timing for specimen collection for Cellophane Tape Preparation?

Before patient washes/defecates Signup and view all the answers

Which parasite detection method involves using saline wet preps as the method of choice?

Saline wet preps Signup and view all the answers

Which parasite's trophozoites can be detected using Duodenal Material?

Giardia intestinalis Signup and view all the answers

The Knott technique involves concentrating blood with low microfilariae numbers by mixing venipuncture-collected blood with what substance?

2% formalin Signup and view all the answers

What is the purpose of culturing Naegleria or Acanthamoeba on non-nutrient agar seeded with Escherichia coli?

Detecting Naegleria fowleri or Acanthamoeba Signup and view all the answers

Which specimen is primarily used for examining Acanthamoeba?

Eye specimens Signup and view all the answers

Which specimen type is helpful for detecting Entamoeba histolytica in the colon?

Cultures Signup and view all the answers

What is the main purpose of using Buffy coat slides stained with Giemsa?

To detect Leishmania & Trypanosoma Signup and view all the answers

What can be recovered from Novy-MacNeal-Nicolle (NNN) medium?

Coccidian parasites and microsporidia Signup and view all the answers

Which technique uses thick slides Giemsa-stained from resulting sediment?

Knott technique Signup and view all the answers