Parasitology Specimen Collection Guidelines
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Questions and Answers

What is the recommended number of specimens for detecting protozoa and helminths in stool?

  • 3 specimens (correct)
  • 2 specimens
  • 4 specimens
  • 1 specimen
  • What is the proper fixative to use for preserving protozoan cysts?

  • Barium
  • Formalin (correct)
  • Mineral oil
  • Bismuth
  • Why should specimens be fixed in preservative for at least 30 minutes before processing?

  • To reduce the shelf-life of the specimen
  • To enhance the color of the specimen
  • To preserve protozoa morphology (correct)
  • To halt helminth egg development
  • Which substance is listed as interfering with stool specimen testing?

    <p>Mineral oil</p> Signup and view all the answers

    When should stool samples be collected for testing?

    <p>Before therapy or 5 to 7 days after therapy</p> Signup and view all the answers

    What are the advantages of formalin as a fixative?

    <p>Easy to prepare and preserves specimens for several years</p> Signup and view all the answers

    Which technique is used for detecting Enterobius vermicularis eggs?

    <p>Cellophane Tape Preparation</p> Signup and view all the answers

    What is the recommended staining method for blood smears prepared from fresh whole blood without anticoagulant?

    <p>Wright’s stain</p> Signup and view all the answers

    What is the recommended timing for specimen collection for Cellophane Tape Preparation?

    <p>Before patient washes/defecates</p> Signup and view all the answers

    Which parasite detection method involves using saline wet preps as the method of choice?

    <p>Saline wet preps</p> Signup and view all the answers

    Which parasite's trophozoites can be detected using Duodenal Material?

    <p>Giardia intestinalis</p> Signup and view all the answers

    The Knott technique involves concentrating blood with low microfilariae numbers by mixing venipuncture-collected blood with what substance?

    <p>2% formalin</p> Signup and view all the answers

    What is the purpose of culturing Naegleria or Acanthamoeba on non-nutrient agar seeded with Escherichia coli?

    <p>Detecting Naegleria fowleri or Acanthamoeba</p> Signup and view all the answers

    Which specimen is primarily used for examining Acanthamoeba?

    <p>Eye specimens</p> Signup and view all the answers

    Which specimen type is helpful for detecting Entamoeba histolytica in the colon?

    <p>Cultures</p> Signup and view all the answers

    What is the main purpose of using Buffy coat slides stained with Giemsa?

    <p>To detect Leishmania &amp; Trypanosoma</p> Signup and view all the answers

    What can be recovered from Novy-MacNeal-Nicolle (NNN) medium?

    <p>Coccidian parasites and microsporidia</p> Signup and view all the answers

    Which technique uses thick slides Giemsa-stained from resulting sediment?

    <p>Knott technique</p> Signup and view all the answers

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