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MOTILITY: progressive fingerlike

KARYOSOME: small and central

PERIPHERAL CHROMATIN: fine and evenly distributed

CYTOPLASM: finely granulate

CYTOPLASMIC INCLUSIONS *OTHER INFOS: ingested RBCs (only diff between E. dispar)

Entamoeba HISTOLYTICA

MOTILITY: non progressive fingerlike KARYOSOME: small and central PERIPHERAL CHROMATIN: fine and evenly distributed CYTOPLASM: finely granulate CYTOPLASMIC INCLUSIONS OTHER INFOS: ingested bacteria

Entamoeba HARTMANNI

MOTILITY: sluglike, blunt KARYOSOME: large and usually centrally located PERIPHERAL CHROMATIN: absent CYTOPLASM: granular and usually vacuolated CYTOPLASMIC INCLUSIONS *OTHER INFOS: *caused PAM: primary amoebic meningoencephalitis

NAEGLERIA FOWLERI

MOTILITY: non progressive blunt KARYOSOME: large, irregular, and eccentric PERIPHERAL CHROMATIN: unevenly distributed CYTOPLASM: coarse and granulated CYTOPLASMIC INCLUSIONS *OTHER INFOS: vacuoles containing bacteria

Entamoeba COLI

MOTILITY: normal stool: sluggish, non progressive / diarrheal stool: progressive, unidirectional KARYOSOME: small and central PERIPHERAL CHROMATIN: fine and evenly distributed CYTOPLASM: granular and vacuolated CYTOPLASMIC INCLUSIONS *OTHER INFOS: ingested bacteria and other food particles

Entamoeba POLECKI

MOTILITY: sluggish, spinelike KARYOSOME: large PERIPHERAL CHROMATIN: granular and vacuolated CYTOPLASM: granular and usually vacuolated CYTOPLASMIC INCLUSIONS *OTHER INFOS: *caused PAM: *caused GAE: granulomatous amoebic encephalitis and some eye infections

ACANTHAMOEBA SPP

MOTILITY: active, varying pseudopod appearance KARYOSOME: centrally located PERIPHERAL CHROMATIN: fine and evenly distributed CYTOPLASM: finely granulated CYTOPLASMIC INCLUSIONS *OTHER INFOS: leaukocytes, epithelial cells and bacteria *examined through mouth scrapings

Entamoeba GINGIVALIS

MOTILITY: sluggish, non progressive blunt KARYOSOME: large, irregular, and blotlike PERIPHERAL CHROMATIN: absent CYTOPLASM: granular and vacuolated CYTOPLASMIC INCLUSIONS *OTHER INFOS: bacteria

ENDOLIMAX NANA

MOTILITY: sluggish, usually progressive KARYOSOME: large, usually central refractive acromatic granules may/not be present PERIPHERAL CHROMATIN: absent CYTOPLASM: coarsely granular and vacuolated CYTOPLASMIC INCLUSIONS *OTHER INFOS: bacteria, yeast cells and other debris

IODAMOEBA BUSTCHLII

Balantidium coli

Trophozoite = Rotary, boring Trophozoite = Two Kidney-shaped macronucleus Small spherical micronucleus Cyst = Two Kidney-shaped macronucleus - usually present Small spherical micronucleus; may not be observable Cyst = One or two visible contractile vacuoles in young cysts; Double cyst wall; Row of cilia visible in between cyst wall layers of young cysts

Young oocyst: Two sporoblast Mature oocyst: Two sporocysts, each containing four sausage-shaped sporozoites

sospora belli

Centrally located Fluid-filled structure Consumes almost 90% of organism

Blastocystis hominis

Study Notes

Motility and Characteristics of Amoeba Species

  • E. dispar: Motility is non-progressive, fingerlike; karyosome is small and central; peripheral chromatin is fine and evenly distributed; cytoplasm is finely granulate; cytoplasmic inclusions are ingested RBCs.

  • E. histolytica: Motility is sluglike, blunt; karyosome is large and usually centrally located; peripheral chromatin is absent; cytoplasm is granular and usually vacuolated; cytoplasmic inclusions are notable for causing PAM (primary amoebic meningoencephalitis).

  • E. hartmanni: Motility is non-progressive, blunt; karyosome is large, irregular, and eccentric; peripheral chromatin is unevenly distributed; cytoplasm is coarse and granulated; cytoplasmic inclusions are vacuoles containing bacteria.

  • E. coli: Motility is normal in stool (sluggish, non-progressive) and diarrheal stool (progressive, unidirectional); karyosome is small and central; peripheral chromatin is fine and evenly distributed; cytoplasm is granular and vacuolated; cytoplasmic inclusions are ingested bacteria and other food particles.

  • Acanthamoeba: Motility is sluggish, spinelike; karyosome is large; peripheral chromatin is granular and vacuolated; cytoplasm is granular and usually vacuolated; cytoplasmic inclusions are notable for causing PAM (primary amoebic meningoencephalitis) and GAE (granulomatous amoebic encephalitis) and some eye infections.

  • Naegleria: Motility is active, varying pseudopod appearance; karyosome is centrally located; peripheral chromatin is fine and evenly distributed; cytoplasm is finely granulated; cytoplasmic inclusions are leukocytes, epithelial cells, and bacteria, which can be examined through mouth scrapings.

  • Balamuthia: Motility is sluggish, non-progressive blunt; karyosome is large, irregular, and blotlike; peripheral chromatin is absent; cytoplasm is granular and vacuolated; cytoplasmic inclusions are bacteria.

  • Free-living Amoeba: Motility is sluggish, usually progressive; karyosome is large, usually central, with refractive acromatic granules that may or may not be present; peripheral chromatin is absent; cytoplasm is coarsely granular and vacuolated; cytoplasmic inclusions are bacteria, yeast cells, and other debris.

Balantidium coli

  • Young oocyst has two sporoblasts; mature oocyst has two sporocysts, each containing four sausage-shaped sporozoites.
  • Centrally located fluid-filled structure consumes almost 90% of the organism.

Identify the characteristics of Entamoeba, a genus of parasitic amoebae, including their motility, karyosome, peripheral chromatin, and cytoplasm. Learn about the distinguishing features of E. dispar and more.

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