Pancreatic Islet Cell Destruction Quiz

Which method is used to destroy the glucagon-secreting cells in the pancreas?

Treatment with alloxan

What does cobalt chloride treatment target in the pancreas?

Glucagon-secreting cells

Which nerve disruption is carried out to affect gastrin production in the stomach?

Deafferentation Vagotomy

What is the function of gastrin in the stomach?

Stimulating HCl and pepsinogen secretion Signup and view all the answers

Why is it necessary to use highly inbred strains when transplanting tissues?

To minimize problems related to tissue rejection Signup and view all the answers

What is the purpose of Parabiosis as described in the text?

To connect blood supplies of two animals Signup and view all the answers

What is a crucial consideration when conducting whole animal studies with hormones?

The appropriate physiological state of the animal Signup and view all the answers

In whole animal studies, why is it important to determine whether the dose of hormone used is physiological or pharmacological?

To assess if the dose can affect the animal's metabolism Signup and view all the answers

What is a major advantage of using an isolated perfused organ system in studies on hormone effects?

It provides a controlled environment to study organ metabolism Signup and view all the answers

Why are potential interactions or side effects of hormone treatments important considerations in whole animal studies?

To identify any unexpected effects that may influence the results Signup and view all the answers

What factor plays a key role in determining the appropriate animal model for whole animal studies on hormones?

The size of the animal model relative to the study requirements Signup and view all the answers

Why is the route of administration an important consideration in hormone studies?

To assess how the method of delivery affects hormone distribution and effects Signup and view all the answers

Which method involves placing catheters in the blood vessels supplying and draining an organ or tissue without removing it from the animal?

Isolated organ or tissue in situ Signup and view all the answers

In the split-udder design for dairy cattle, why are separate catheters placed in the external pudental arteries supplying the mammary gland?

To prevent treatment effects on one side Signup and view all the answers

What factors should be considered in the organ perfusion/infusion model?

Determining the appropriate dose and treatment duration Signup and view all the answers

What is a key advantage of using isolated organs or tissues in situ for experimentation?

Easy manipulation and sampling Signup and view all the answers

Why are blood samples taken from arterial and venous catheters in the isolated organ or tissue in situ model?

To determine hormone levels and effects on metabolites Signup and view all the answers

What is the purpose of supplying test compounds via arterial catheters in the isolated organ or tissue in situ model?

To observe effects on the organ directly Signup and view all the answers

What is a limitation of in vitro models when studying the tissue being studied?

The results are qualitative rather than quantitative Signup and view all the answers

How can the results of in vitro models be affected by the incubation medium?

The presence of particular hormones or metabolites can alter metabolic pathways Signup and view all the answers

What is a common issue with the concentration of hormones used in in vitro models?

The concentration may be pharmacological rather than physiological Signup and view all the answers

What is an advantage of using thin tissue slices in in vitro models?

Slices must be thin to allow oxygen penetration to interior cells Signup and view all the answers

How does using thin tissue slices compare to perfusion in terms of technical ease?

Both methods have similar technical difficulty levels Signup and view all the answers

What is a challenge associated with using thin tissue slices in in vitro models?

Many damaged cells may be present on the surface of the slice Signup and view all the answers

What is the main advantage of using immortalized cell lines in experiments?

They offer a stable long-term system for experiments Signup and view all the answers

Which of the following conditions is typically required for cell culture using immortalized cell lines?

Defined medium with serum Signup and view all the answers

What is a key consideration when determining the appropriate duration of treatment in cell culture experiments?

Maintaining cell viability and hormone receptors Signup and view all the answers

Which type of inhibitors can be used to determine the mechanism of action of hormones in cell culture studies?

Inhibitors of DNA, RNA, and protein synthesis Signup and view all the answers

What does caution should researchers take when interpreting results obtained using immortalized cell lines?

Results from immortalized cell lines may not reflect those from primary cells Signup and view all the answers

In what way do immortalized cell lines differ from freshly isolated primary cells?

Immortalized cell lines may not maintain all properties of primary cells Signup and view all the answers