Oxidative and Fermentation Tests in Microbiology Quiz
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Questions and Answers

What is the purpose of the O/F Glucose test?

  • To test for the presence of peptone in the organism
  • To identify the specific sugars fermented by the organism
  • To determine if the organism is oxidative or fermentative (correct)
  • To establish anaerobic conditions for the organism
  • What does a color change from green to yellow in the O/F Glucose test indicate?

  • Positive test for fermentation (correct)
  • Presence of peptone in the organism
  • Positive test for respiration
  • Absence of glucose in the medium
  • What is the purpose of layering one tube with mineral oil in the O/F Glucose test?

  • To provide additional nutrients for the organism
  • To establish anaerobic conditions (correct)
  • To prevent the diffusion of glucose
  • To promote the growth of aerobic bacteria
  • What are the three important ingredients in the O/F Glucose medium?

    <p>Glucose, peptone, brom-thymol blue</p> Signup and view all the answers

    Why is E coli used as a control organism in the O/F Glucose test?

    <p>To represent a facultative organism</p> Signup and view all the answers

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