Overview of Pipettes
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Questions and Answers

What is a TC pipette designed to do?

Contain a specific volume of liquid but does not dispense that exact volume.

What does a TD pipette do?

Dispense a specified volume.

A blowout pipette should dispense the last drop of liquid completely.

True

What is the main purpose of transfer pipettes?

<p>To transfer a known volume of liquid without further subdivisions.</p> Signup and view all the answers

What distinguishes a volumetric pipette from an Ostwald-Folin pipette?

<p>The bulb position; volumetric has a bulb in the center, while Ostwald-Folin has a bulb closer to the delivery tip.</p> Signup and view all the answers

Which type of pipette has an etched ring near the mount piece indicating it is a blowout pipette?

<p>Ostwald-Folin pipette</p> Signup and view all the answers

A measuring pipette is not considered sufficiently accurate for measuring samples and calibrators.

<p>True</p> Signup and view all the answers

What distinguishes the Mohr pipette from the Serological pipette?

<p>Serological pipette drains faster.</p> Signup and view all the answers

What is the main function of a micropipette?

<p>To measure less than 1 mL.</p> Signup and view all the answers

Study Notes

Overview of Pipettes

  • Pipettes are essential tools for accurately transferring measured volumes of liquid.
  • Two primary categories: TC (To Contain) pipettes and TD (To Deliver) pipettes.
  • TC pipets contain a specific volume but do not dispense it; TD pipets deliver the indicated volume.

Types of Pipettes

  • Blowout Pipettes

    • Identified by continuous etched rings (one or two) near the top.
    • Indicate that the last drop must be expelled into the receiving vessel for accurate delivery.
  • Self-Draining Pipettes

    • Rely on gravity to drain liquid, not requiring additional expulsion.

Transfer Pipettes

  • Designed to transfer a known volume without subdivisions.
  • Includes Volumetric and Ostwald-Folin pipettes.
  • Characterized by a cylindrical bulb connecting to narrow tubing with a calibration mark for accuracy.

Volumetric Transfer Pipette

  • Calibrated for precise delivery of a fixed volume, primarily used for diluting solutions.
  • Self-draining design enables efficient use in preparing standards or quality control materials.

Ostwald-Folin Pipette

  • Features a bulb positioned closer to the delivery tip, suitable for measuring viscous liquids like blood or serum.
  • Contains an etched ring to indicate it is a blowout pipette and allows complete drainage after liquid has flowed down.

Measuring Pipettes

  • Graduated pipettes that can dispense various volumes due to multiple graduation marks.

  • Not highly accurate for calibrators but useful for transferring reagents and making dilutions.

  • Mohr Pipette

    • Graduations end above the tip; self-draining mechanism.
  • Serological Pipette

    • Graduation marks reach down to the tip and marked with an etched ring to indicate a blowout design, draining faster due to larger orifice.

Micropipette

  • Special category of measuring pipette designed for volumes less than 1 mL, providing precision in small measurements.

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Description

This quiz covers the essential concepts and types of pipettes used in laboratory settings. Learn about TC and TD pipettes, blowout and self-draining types, and the specifics of volumetric transfer pipettes. Test your knowledge on the characteristics and applications of these important liquid transfer tools.

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