Questions and Answers
What is a TC pipette designed to do?
Contain a specific volume of liquid but does not dispense that exact volume.
What does a TD pipette do?
Dispense a specified volume.
A blowout pipette should dispense the last drop of liquid completely.
True
What is the main purpose of transfer pipettes?
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What distinguishes a volumetric pipette from an Ostwald-Folin pipette?
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Which type of pipette has an etched ring near the mount piece indicating it is a blowout pipette?
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A measuring pipette is not considered sufficiently accurate for measuring samples and calibrators.
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What distinguishes the Mohr pipette from the Serological pipette?
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What is the main function of a micropipette?
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Study Notes
Overview of Pipettes
- Pipettes are essential tools for accurately transferring measured volumes of liquid.
- Two primary categories: TC (To Contain) pipettes and TD (To Deliver) pipettes.
- TC pipets contain a specific volume but do not dispense it; TD pipets deliver the indicated volume.
Types of Pipettes
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Blowout Pipettes
- Identified by continuous etched rings (one or two) near the top.
- Indicate that the last drop must be expelled into the receiving vessel for accurate delivery.
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Self-Draining Pipettes
- Rely on gravity to drain liquid, not requiring additional expulsion.
Transfer Pipettes
- Designed to transfer a known volume without subdivisions.
- Includes Volumetric and Ostwald-Folin pipettes.
- Characterized by a cylindrical bulb connecting to narrow tubing with a calibration mark for accuracy.
Volumetric Transfer Pipette
- Calibrated for precise delivery of a fixed volume, primarily used for diluting solutions.
- Self-draining design enables efficient use in preparing standards or quality control materials.
Ostwald-Folin Pipette
- Features a bulb positioned closer to the delivery tip, suitable for measuring viscous liquids like blood or serum.
- Contains an etched ring to indicate it is a blowout pipette and allows complete drainage after liquid has flowed down.
Measuring Pipettes
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Graduated pipettes that can dispense various volumes due to multiple graduation marks.
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Not highly accurate for calibrators but useful for transferring reagents and making dilutions.
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Mohr Pipette
- Graduations end above the tip; self-draining mechanism.
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Serological Pipette
- Graduation marks reach down to the tip and marked with an etched ring to indicate a blowout design, draining faster due to larger orifice.
Micropipette
- Special category of measuring pipette designed for volumes less than 1 mL, providing precision in small measurements.
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Description
This quiz covers the essential concepts and types of pipettes used in laboratory settings. Learn about TC and TD pipettes, blowout and self-draining types, and the specifics of volumetric transfer pipettes. Test your knowledge on the characteristics and applications of these important liquid transfer tools.
