Pipettes and Their Types Quiz
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Pipettes and Their Types Quiz

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Questions and Answers

Which type of balance is specifically used for the preparation of primary standards?

  • Spring balance
  • Electronic balance
  • Analytic balance (correct)
  • Mechanical balance

    • An electronic balance can provide measurements that are less precise than a mechanical balance.

    False

    What is the primary purpose of a centrifuge in the laboratory?

    To separate phases of different mass and densities using centrifugal force.

    The ______ measures the degree of acidity or alkalinity of a solution.

    <p>pH meter</p> Signup and view all the answers

    Match the following laboratory instruments with their primary uses:

    <p>Analytic balance = Preparation of primary standards pH meter = Measuring acidity or alkalinity Centrifuge = Separating substances based on density Thermometer = Monitoring temperature</p> Signup and view all the answers

    What feature allows the analytic balance to protect its measurements?

    <p>Transparent sliding door</p> Signup and view all the answers

    Most chemistry laboratories have shifted to __________ prepared reagents instead of preparing them from scratch.

    <p>commercially</p> Signup and view all the answers

    Laboratory refrigerators do not typically require thermometers.

    <p>False</p> Signup and view all the answers

    What is the primary purpose of Type I reagent water?

    <p>Used for procedures requiring maximum purity</p> Signup and view all the answers

    Technical or commercial grade chemicals are suitable for clinical laboratory reagent preparation.

    <p>False</p> Signup and view all the answers

    What is a primary standard?

    <p>A highly purified chemical that can be measured directly to produce a substance of exact known concentration of purity.</p> Signup and view all the answers

    Buffers are weak acids or bases and their related salts that minimize changes in the ______ ion concentration.

    <p>hydrogen</p> Signup and view all the answers

    Match the types of reagent water with their primary usage:

    <p>Type I = Maximum purity for critical procedures Type II = Qualitative chemistry procedures and hematology Type III = General urinalysis tests and washing glassware</p> Signup and view all the answers

    Pasteur pipets have a reusable suction top and a disposable glass bottom.

    <p>True</p> Signup and view all the answers

    What is a capillary pipet primarily made of?

    <p>Capillary tubing</p> Signup and view all the answers

    The surface of the liquid in a pipette creates a __________, which is observed as the curvature of the liquid surface.

    <p>meniscus</p> Signup and view all the answers

    What is the purpose of the calibration mark on a pipet?

    <p>To indicate the specified volume to be measured.</p> Signup and view all the answers

    When using a pipette, how should the delivery tip be positioned during drainage?

    <p>Held vertically against the inside of the vessel</p> Signup and view all the answers

    Match the following types of pipets with their characteristics:

    <p>Capillary pipets = Disposable, calibrated for specific volumes Pasteur pipets = Reusable suction top with disposable glass bottom Disposable transfer pipets = Plastic construction, single-use Manual pipets = Require manual control for liquid transfer</p> Signup and view all the answers

    You should completely remove your index finger from the suction opening to allow free drainage from the pipette.

    <p>False</p> Signup and view all the answers

    What should be done to the pipette tip before transferring liquid to another vessel?

    <p>Wipe the outside of the pipette dry with gauze or tissue.</p> Signup and view all the answers

    A Volumetric pipette is designed to measure only one specific volume of liquid.

    <p>True</p> Signup and view all the answers

    What is the primary purpose of a micropipette?

    <p>To measure small volumes of liquid, less than 1 mL.</p> Signup and view all the answers

    What type of pipette is known as 'to deliver' and requires that the remaining liquid at the tip must be expelled?

    <p>Serological pipette</p> Signup and view all the answers

    The __________ pipette has a calibration line that is located before the tip, allowing for the measurement of liquids without a blowout.

    <p>Mohr</p> Signup and view all the answers

    Match each pipette type with its characteristic:

    <p>Serological = To deliver (TD)/blowout pipette Volumetric = To contain (TC)/no blowout pipette Ostwald-Folin = TD/blowout pipette used for viscous solutions Micropipette = Measures less than 1 mL</p> Signup and view all the answers

    Which type of pipette is used for measuring viscous solutions such as whole blood?

    <p>Ostwald-Folin pipette</p> Signup and view all the answers

    Micropipettes are always designed as 'to contain' (TC) pipettes.

    <p>False</p> Signup and view all the answers

    Describe the main drainage characteristic of a self-draining pipette.

    <p>Fluid drains by gravity, requiring contact with the receiving container.</p> Signup and view all the answers

    What is the primary advantage of semiautomated pipettes compared to traditional pipetting methods?

    <p>They require no pipetting bulb.</p> Signup and view all the answers

    Plastic tips used in semiautomated pipettes are not autoclavable.

    <p>False</p> Signup and view all the answers

    What step follows attaching the proper tip to the pipettor when using a semiautomated micropipettor?

    <p>Set the delivery volume.</p> Signup and view all the answers

    The high purity chemicals used often in the preparation of reagents are known as ______ grade.

    <p>Analytic reagent</p> Signup and view all the answers

    Match the following reagent grades with their descriptions:

    <p>Analytic Reagent (AR) = High purity for reagent preparation Ultrapure grade = Used in chromatography and other specific procedures Chemically Pure (CP) = Impurity limitations not stated USP and NF grade = Generally less pure, not injurious to health</p> Signup and view all the answers

    Which of the following types of pipets can be used for multichannel pipetting?

    <p>Semiautomated pipets</p> Signup and view all the answers

    Semiautomated micropipettors always require tips to be wiped before dispensing liquid.

    <p>False</p> Signup and view all the answers

    The disposal of used tips in semiautomated pipetting helps minimize ______ exposure.

    <p>biohazard</p> Signup and view all the answers

    Study Notes

    Pipette Types and Functions

    • TD pipette dispenses the indicated volume.
    • Pipette types include Transfer (Volumetric, Ostwald-folin, Pasteur, automatic macropipettes, micropipettes) and Measuring/Graduated (Serologic, Mohr, Bacteriologic, Ball, Kolmer, Kahn, Micropipette).
    • Blowout pipettes have etched rings indicating the remaining liquid must be expelled.
    • Self-draining pipettes allow liquid to drain by gravity while maintaining contact with the receiving vessel.

    Serological and Mohr Pipettes

    • Serological pipet acts as a TD/blowout pipet, calibrated to the tip.
    • Mohr pipet is a TD/"no blowout" pipet; its end-calibration line is before the tip.
    • Volumetric pipets measure a single volume, are TD/"no blowout," and are more accurate than serological pipets.
    • Ostwald-Folin pipet is a TD/blowout pipet for viscous solutions, less commonly used today.

    Micropipettes

    • Micropipettes measure small volumes (less than 1 mL) and are typically TC pipets.
    • Unopette is a self-filling micropipette used in hematology.
    • Capillary pipets are disposable TC micropipettes made from capillary tubing.

    Pasteur and Disposable Transfer Pipets

    • Pasteur pipets are reusable with a disposable glass bottom; disposable pipets are plastic.
    • Mechanical pipetting involves checking size, holding correctly, and using a bulb to draw liquid.

    Manual Pipetting Steps

    • Use suction to fill the pipet above calibration mark, cover the opening with a finger.
    • Wipe excess fluid away, touch the tip to the receiving vessel, and allow gravity drainage to the calibration mark.
    • Complete drainage may require touching the tip to different areas of the receiving vessel.

    Laboratory Equipment Essentials

    • A laboratory balance ensures accurate reagent preparation and is fundamental for experiments.
    • Analytic balances measure from 0.01 mg to 160 g; electronic balances provide rapid and precise measurements.
    • pH meters express acidity or alkalinity, with values displayed digitally or on scales.

    Centrifuges

    • Centrifuges separate phases based on mass and density, composed of a head or rotor and enclosed within a metal cover.

    Semiautomated Pipettes

    • Semiautomated pipettes enhance convenience, using plastic tips that reduce contamination risks.
    • Tips are disposable or autoclavable and can be single or multichannels, operated by a trigger or plunger.

    Reagents and Chemical Types

    • Reagents facilitate chemical reactions; they can come in ready-to-use forms or kits.
    • Analytic reagent (AR) grade chemicals boast high purity, while ultrapure grade serves specialized procedures.
    • Chemically Pure (CP) grade lacks precise impurity limits; USP and NF grades are intended for health safety, not purity.

    Reference and Reagent Water

    • Primary standards are highly purified chemicals with known concentration; certified by NIST.
    • Type I reagent water is extremely pure, used immediately and not to be stored; critical for various analytical techniques.
    • Type II reagent water serves qualitative procedures; Type III is suitable for basic laboratory tests like urinalysis.

    Buffers

    • Buffers are weak acids or bases that stabilize hydrogen ion concentration, minimizing pH changes during reactions.

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    Description

    Test your knowledge on various types of pipettes, including volumetric and graduated options. This quiz covers the characteristics and uses of different pipettes such as Ostwald-Folin, Pasteur, and automatic micropipettes. Perfect for anyone studying laboratory techniques.

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