Types-of-pipette-ppt-student.pdf

Types of pipette Prof Sheila Grace A.Martin  Outline of Pipette lecture PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  Pipettes...

Types of pipette Prof Sheila Grace A.Martin  Outline of Pipette lecture PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  Pipettes these are used to transfer a known volume of liquid from one container to another. designed either to contain (TC) a specific volume of liquid or to deliver (TD) a specified volume. - TC pipet holds or contains a particular volume but does not dispense that exact volume. TD pipet will dispense that exact volume indicated. PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  Pipettes  Blowout - continuous etched ring or two small, close continuous rings located near the top of the pipet. -means that the last drop of liquid should be expelled into the receiving vessel. Without these markings. Self-draining - a pipet is self-draining, and content of pipet drain by gravity. PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  Pipettes  Transfer pipettes: I -It transfers a known volume of liquid without further subdivisions. They include both volumetric and Ostwald-Folin pipettes. They consist of a cylindrical bulb joined at both ends to narrower glass tubing. A calibration mark is etched around the upper suction tube. The bore of delivery orifice should be sufficiently narrow to prevent rapid outflow of liquid and incomplete drainage. PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  volumetric transfer pipette -calibrated to deliver accurately a fixed volume of a dilute aqueous solution. -self draining and is used in diluting standards, calibrators, or quality-control materials. Ostwald-Folin pipettes - have bulb closer to the delivery tip unlike volumetric pipette which has at center. -used for accurate measurement of viscous fluids, such as blood or serum. -has an etched ring near the mount piece, indicating it is a blow out pipette. - with the use of pipetting bulb, liquid is blown out of the pipette only after blood or serum has drained to the last drop in delivery tip. PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  Measuring pipette: -graduated or measuring pipette and can dispense different volumes as these have many graduations. - uniform graduations along its length. - used for the transferring and measurement of reagents and making dilutions and are not considered sufficiently accurate for measuring samples and calibrators. Two kinds are available; 1.Mohr pipette has graduations ends above the tip (self draining) 2.Serological pipette has graduated marks down to the tip. ( blow out)/ etched ring (or pair of rings) near/ larger orifice / drain faster Micropippet is also a measuring pippet which can measure less than 1mL. PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson  Types of pipette PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson PROF. SHEILA GRACE A. MARTIN, RMT, MSc CHE 000L NO 4. Special lesson