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Questions and Answers
What is the main principle behind the method described in the text?
In which type of solution does hemolysis (bursting) occur for RBC?
What is the formula for calculating osmolality?
Which substance has 3 osmotically active particles?
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At what NaCl concentration does hemolysis normally start?
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In which condition does hemolysis start earlier than normal?
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What is osmotic fragility testing used for?
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What does osmosis refer to?
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What happens to red blood cells in a hypertonic solution?
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What is the process of moving particles from a region of higher concentration to a region of lower concentration called?
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In which condition will red blood cells swell up?
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What does osmotic fragility test indicate when hemolysis occurs sooner?
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Study Notes
Osmolality and Hemolysis
- The main principle behind the method described is the concept of osmosis, which refers to the movement of water molecules from a region of higher concentration to a region of lower concentration.
Hemolysis of RBCs
- Hemolysis (bursting) of Red Blood Cells (RBCs) occurs in a hypotonic solution.
- Hemolysis normally starts at a NaCl concentration of around 0.48%.
Calculating Osmolality
- The formula for calculating osmolality is: osmolality = 2 × (Na+ + K+) + glucose + urea (all in mmol/L)
Osmotically Active Particles
- Sucrose has 3 osmotically active particles.
Osmotic Fragility Testing
- Osmotic fragility testing is used to diagnose hereditary spherocytosis and other red blood cell disorders.
- Osmotic fragility test indicates when hemolysis occurs sooner, it may indicate a fragile red blood cell membrane.
Effects of Solutions on RBCs
- In a hypertonic solution, red blood cells shrink.
- In a hypotonic solution, red blood cells swell up.
- The process of moving particles from a region of higher concentration to a region of lower concentration is called diffusion.
Abnormal Conditions
- In hereditary spherocytosis, hemolysis starts earlier than normal.
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Description
Test your knowledge about osmotic fragility and osmosis with this quiz. Learn about the measurement of red blood cell resistance to hemolysis and the process of osmosis in cells when suspended in different saline solutions.
