10 Questions
What is the recommended time frame for carrying out the test after blood collection?
Within 2 hours, or within 6 hours if kept at 4 C.
What is the purpose of preparing different concentrations of NaCl in the test tubes?
To determine osmotic fragility by observing lysis at different NaCl concentrations.
How much blood should be added to each test tube?
50 uL (microlitres).
What is the next step after incubating the tubes at room temperature for 30 minutes?
Mix again and centrifuge.
How is the amount of lysis estimated in this test?
Using a colorimeter at 540 nm.
What concentration of NaCl is assigned a value of 100% lysis?
Tube 10 (water), which has 0% NaCl.
What range of NaCl concentration is considered as the normal range for initial lysis in osmotic fragility?
0.45 - 0.50 % NaCl.
What range of NaCl concentration is considered as the normal range for complete lysis in osmotic fragility?
0.20 - 0.33 % NaCl.
What is the significance of recording the Median Corpuscular Fragility (MCF)?
To determine the concentration of saline causing 50% lysis.
What conditions lead to the entire osmotic fragility curve shifting to the right?
Increased osmotic fragility; Hereditary spherocytosis (HS) and Autoimmune hemolytic anaemia (AIHA).
Test your knowledge on the osmotic fragility test for erythrocytes in this practical session quiz. Understand the concept of osmosis and the tendency of red blood cells to hemolyse. Prepared for fourth-year students on February 1, 2024, by Dr. Salar Ayob Sulaiman.
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