Nutritional Microbiology Practical Lab Safety

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What is a unique aspect of the microbiology laboratory?

Conducting experiments in a vacuum
Dealing with dead organisms
Mixing chemicals and reagents
Dealing with live organisms (correct)

What is the primary purpose of microbiological media?

To isolate specific microorganisms
To serve as a nutrient base for microorganisms (correct)
To reduce contamination
To provide a favourable environment for microorganisms

What is the category of microorganisms that poses a very high risk to individual and community?

Category 2
Category 3
Category 1
Category 4 (correct)

What is the purpose of inoculation in culturing techniques?

To transfer microbial cells from a test sample to a fresh medium (B) Signup and view all the answers

What is the recommended practice for handling reagents in the microbiology laboratory?

Attending to the description of reagents and hazards (B) Signup and view all the answers

Why is it important to put hair and nails in order in the microbiology laboratory?

To ensure personal safety (B) Signup and view all the answers

What is the purpose of incubation in microbiology?

To allow growth of microbial species (D) Signup and view all the answers

What is the primary goal of isolation in microbiology?

To separate single species of microorganisms from populations (A) Signup and view all the answers

What is the temperature range for incubating cultures from food origin?

30-37°C (A) Signup and view all the answers

What is the purpose of inspection in microbiology?

To identify microorganisms based on cultural properties (D) Signup and view all the answers

What technique is employed for rapid qualitative isolation of microorganisms?

Streak-plate technique (D) Signup and view all the answers

What test is being performed to identify microorganisms in this practical?

Indole production test for E (C) Signup and view all the answers

What is the primary function of the primary stain in the Gram stain procedure?

To impart its colour to all cells (D) Signup and view all the answers

What is the purpose of the decolourising agent in the Gram stain procedure?

To establish a colour contrast by removing the primary stain from certain cells (C) Signup and view all the answers

What is the consequence of the primary stain not being removed from the cells?

The counter stain will not be able to stain the cells (B) Signup and view all the answers

What is the function of the mordant in the Gram stain procedure?

To intensify the colour of the primary stain (C) Signup and view all the answers

What is the purpose of the counter stain in the Gram stain procedure?

To provide a contrasting colour to the primary stain (C) Signup and view all the answers

How many chemical reagents are used in the Gram stain procedure?

At least four (A) Signup and view all the answers

What is the outcome of a Gram staining procedure for E. coli?

It retains the counterstain (safranin) (B) Signup and view all the answers

What is the purpose of heat-fixing microbial cells?

To enable the cells to be viewed under the microscope (C) Signup and view all the answers

What is the main purpose of microscopic observation in microbiology?

To observe the morphology of microbial cells (B) Signup and view all the answers

What is the specific reagent used to detect indole production in E. coli?

Kovac's reagent (B) Signup and view all the answers

What is the consequence of tryptophan hydrolysis in the indole production test?

Production of a cherry-like red layer (A) Signup and view all the answers

What is the purpose of the indole production test in microbiology?

To identify E. coli from other bacteria (B) Signup and view all the answers