Podcast
Questions and Answers
What should you do in case of spills or broken glassware in the laboratory?
What should you do in case of spills or broken glassware in the laboratory?
What is the main purpose of culture media in microbiology?
What is the main purpose of culture media in microbiology?
What is the primary purpose of wearing personal protective equipment in a micro lab?
What is the primary purpose of wearing personal protective equipment in a micro lab?
Why is aseptic technique important in transferring bacteria?
Why is aseptic technique important in transferring bacteria?
Signup and view all the answers
What is the purpose of selective media?
What is the purpose of selective media?
Signup and view all the answers
What is the term for a solid culture media that has been solidified with agar?
What is the term for a solid culture media that has been solidified with agar?
Signup and view all the answers
What should you do if you spill something in the laboratory?
What should you do if you spill something in the laboratory?
Signup and view all the answers
What should you do with bacterial cultures and materials that have come into contact with bacteria?
What should you do with bacterial cultures and materials that have come into contact with bacteria?
Signup and view all the answers
Why is it important to wear closed-toed shoes and long pants in the laboratory?
Why is it important to wear closed-toed shoes and long pants in the laboratory?
Signup and view all the answers
What should you do at the end of the laboratory period?
What should you do at the end of the laboratory period?
Signup and view all the answers
Study Notes
Lab Safety in Micro Lab
- The basic rule is to eliminate exposure to harmful pathogens or chemicals via proper personal protective equipment, airflow, and waste management.
- Proper personal protective equipment includes:
- Close-toed shoes and long pants
- Tying back long hair or bangs
- Gloves (available and to be worn when indicated)
- Lab coats (must be worn at all times)
- Safety glasses or goggles (available)
- Proper waste management involves:
- Autoclaving bacterial cultures and items that have touched bacteria
- Disposing of petri dishes with media and cultures in special areas
- Separating waste by bacterial exposure
- Disposing of glass in special containers
- Disposing of weigh boats, paper towels, and other non-bacterial items in regular trash
Lab Procedures
- At the end of the lab period:
- Put away all used equipment in proper places
- Clear and wipe down benches with disinfectant
- Clean up common areas used
- Wash hands before eating or interacting with others
- Report all spills and broken glassware to the instructor for cleanup instructions
- Know the location of emergency equipment, including:
- Eye wash
- Fire alarms
- Fire extinguishers
- Chemical safety showers
- Telephones and emergency numbers
The Microbiology Laboratory and Cultivation of Bacteria
Introduction to Culture Media
- Culture media provides nutrients for growing microorganisms
- Classification of media by:
- Consistency: liquid (broth) or solid (agar)
- Type: nutrient, selective, or differential media
- Nutrient media:
- Provides all necessary nutrients and minerals for microorganism growth
- Can be defined (exact composition known) or undefined (exact composition unknown)
- Selective media:
- Favors growth of specific microorganisms over others
- May inhibit growth of other microorganisms
- Differential media:
- Recognizes specific microorganisms through biochemical reactions
- Often uses color changes to distinguish microorganisms
Aseptic Technique in Transferring Bacteria
- Aseptic technique is used to separate microorganisms from mixed microbial populations
- Involves introducing a small sample of cells (inoculum) into a receptacle containing nutrient or culture medium
- Aseptic technique is designed to prevent contamination of microorganisms and equipment
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
This quiz covers the essential rules and guidelines for ensuring safety in a microbiology laboratory, including proper personal protective equipment, airflow, and waste management.
