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Nucleic Acid Sequence Identification by Hybridization

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Nucleic Acid Sequence Identification by Hybridization

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Questions and Answers

What is the temperature at which the iterative process takes place in Strand Displacement Amplification?

40°C

52°C (correct)

60°C

72°C

What is the result of adding a fluorogenic probe in Strand Displacement Amplification?

It produces a fluorescent signal (correct)

It has no effect on the amplification process

It inhibits the amplification process

It decreases the amount of amplified probe

What is the basis of the BD ProbeTec ET for Mycobacterium tuberculosis?

Branch DNA amplification

Cleavage-based amplification

Strand Displacement Amplification (correct)

Hybrid Capture Assay

What is the main difference between target amplification and signal amplification methods?

The number of target sequences increases in target amplification methods Signup and view all the answers

What is the purpose of the extender probes in branched-chain DNA amplification?

To load target nucleic acids with signal Signup and view all the answers

What is the advantage of signal amplification methods over target amplification methods?

They carry less risk of target contamination Signup and view all the answers

What is the application of branched-chain DNA amplification?

Quantification of target sequences in clinical samples Signup and view all the answers

What is the result of the iterative process in Strand Displacement Amplification?

Millions of copies of the initial sequence are produced Signup and view all the answers

What is the binding target of the oligomer probes in branched-chain DNA amplification?

Target nucleic acids Signup and view all the answers

What is the type of probe amplification method that uses fluorogenic probes?

Strand Displacement Amplification Signup and view all the answers