Nucleic Acid Sequence Identification by Hybridization

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Questions and Answers

What is the temperature at which the iterative process takes place in Strand Displacement Amplification?

  • 40°C
  • 52°C (correct)
  • 60°C
  • 72°C

What is the result of adding a fluorogenic probe in Strand Displacement Amplification?

  • It produces a fluorescent signal (correct)
  • It has no effect on the amplification process
  • It inhibits the amplification process
  • It decreases the amount of amplified probe

What is the basis of the BD ProbeTec ET for Mycobacterium tuberculosis?

  • Branch DNA amplification
  • Cleavage-based amplification
  • Strand Displacement Amplification (correct)
  • Hybrid Capture Assay

What is the main difference between target amplification and signal amplification methods?

<p>The number of target sequences increases in target amplification methods (C)</p> Signup and view all the answers

What is the purpose of the extender probes in branched-chain DNA amplification?

<p>To load target nucleic acids with signal (C)</p> Signup and view all the answers

What is the advantage of signal amplification methods over target amplification methods?

<p>They carry less risk of target contamination (D)</p> Signup and view all the answers

What is the application of branched-chain DNA amplification?

<p>Quantification of target sequences in clinical samples (B)</p> Signup and view all the answers

What is the result of the iterative process in Strand Displacement Amplification?

<p>Millions of copies of the initial sequence are produced (A)</p> Signup and view all the answers

What is the binding target of the oligomer probes in branched-chain DNA amplification?

<p>Target nucleic acids (B)</p> Signup and view all the answers

What is the type of probe amplification method that uses fluorogenic probes?

<p>Strand Displacement Amplification (C)</p> Signup and view all the answers

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