Nucleic Acid Isolation and Extraction Methods

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10 Questions

The opposite strand is simultaneously ______.

displaced

The displaced strand is copied by the ______.

primers

An iterative process takes place at ______ °C without temperature cycling.

52

Addition of fluorogenic probe yields a ______ signal directly proportional to the amount of amplified probe/target.

fluorescent

SDA is the basis for the BD ProbeTec®ET for ______ tuberculosis.

Mycobacterium

Branched-chain ______ is frequently used for quantification of target sequences in clinical samples.

DNA

A series of short oligomer probes captures ______ nucleic acids.

target

Additional extender (or amplifier) probes bind ______ nucleic acids.

target

Multiple reporter molecules load ______ nucleic acid with signal.

target

These systems carry less risk of ______ contamination.

target

Learn about the different methods of isolating and extracting DNA and RNA, including Solid phase extraction and precipitation with isopropyl alcohol. Understand the steps involved in each process and how they help to purify nucleic acids.

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