Podcast
Questions and Answers
What is the primary role of NaCl in DNA extraction?
What is the primary role of NaCl in DNA extraction?
- To denature proteins and dissolve denatured proteins
- To disrupt membranes due to its amphipathic nature
- To solubilize lipid bilayer and remove protein contaminants
- To chelate magnesium ions and inactivate DNAse (correct)
What is the purpose of proteinase K digestion at high temperatures?
What is the purpose of proteinase K digestion at high temperatures?
- To dissolve denatured proteins and remove contaminants
- To break down the cell wall and facilitate cell lysis
- To activate DNAse and initiate DNA degradation
- To unfold and degrade proteins at high temperatures (correct)
Why is SDS used in DNA extraction buffer composition?
Why is SDS used in DNA extraction buffer composition?
- To denature proteins and dissolve denatured proteins
- To solubilize lipid bilayer and remove protein contaminants
- To disrupt membranes due to its amphipathic nature (correct)
- To chelate magnesium ions and inactivate DNAse
What is the role of phenol in DNA extraction?
What is the role of phenol in DNA extraction?
Why is EDTA used in DNA extraction buffer composition?
Why is EDTA used in DNA extraction buffer composition?
What is the purpose of adding 100% ethanol or isopropanol in DNA extraction?
What is the purpose of adding 100% ethanol or isopropanol in DNA extraction?
What is the primary purpose of grinding the sample with liquid nitrogen in DNA extraction from plant cells?
What is the primary purpose of grinding the sample with liquid nitrogen in DNA extraction from plant cells?
What is the role of EDTA in the CTAB extraction buffer?
What is the role of EDTA in the CTAB extraction buffer?
What is the effect of high ionic strength solutions on CTAB?
What is the effect of high ionic strength solutions on CTAB?
Why is NaCl added to the CTAB extraction buffer at concentrations of more than 0.5 M?
Why is NaCl added to the CTAB extraction buffer at concentrations of more than 0.5 M?
What is the purpose of using CTAB in DNA extraction from plant cells?
What is the purpose of using CTAB in DNA extraction from plant cells?
What is the advantage of using CTAB in DNA extraction from organisms that produce large quantities of polysaccharides?
What is the advantage of using CTAB in DNA extraction from organisms that produce large quantities of polysaccharides?
What is the purpose of adding 0.54 volume of ice-cold isopropanol to the sample?
What is the purpose of adding 0.54 volume of ice-cold isopropanol to the sample?
What is the primary function of the 70% ethanol wash step in DNA extraction?
What is the primary function of the 70% ethanol wash step in DNA extraction?
What is the purpose of adding RNase to the sample during DNA extraction?
What is the purpose of adding RNase to the sample during DNA extraction?
What is the primary function of the centrifugation step at 10,000 rpm for 10 minutes?
What is the primary function of the centrifugation step at 10,000 rpm for 10 minutes?
What is the composition of the buffer used to resuspend the DNA pellet?
What is the composition of the buffer used to resuspend the DNA pellet?
Why is it important to leave some of the upper phase behind when transferring the aqueous layer to another tube?
Why is it important to leave some of the upper phase behind when transferring the aqueous layer to another tube?
