DNA Extraction Process

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What is the primary role of NaCl in DNA extraction?

To denature proteins and dissolve denatured proteins

To disrupt membranes due to its amphipathic nature

To solubilize lipid bilayer and remove protein contaminants

To chelate magnesium ions and inactivate DNAse (correct)

What is the purpose of proteinase K digestion at high temperatures?

To dissolve denatured proteins and remove contaminants

To break down the cell wall and facilitate cell lysis

To activate DNAse and initiate DNA degradation

To unfold and degrade proteins at high temperatures (correct)

Why is SDS used in DNA extraction buffer composition?

To denature proteins and dissolve denatured proteins

To solubilize lipid bilayer and remove protein contaminants

To disrupt membranes due to its amphipathic nature (correct)

To chelate magnesium ions and inactivate DNAse

What is the role of phenol in DNA extraction?

To denature proteins and dissolve denatured proteins Signup and view all the answers

Why is EDTA used in DNA extraction buffer composition?

To chelate magnesium ions and inactivate DNAse Signup and view all the answers

What is the purpose of adding 100% ethanol or isopropanol in DNA extraction?

To precipitate DNA and remove impurities Signup and view all the answers

What is the primary purpose of grinding the sample with liquid nitrogen in DNA extraction from plant cells?

To break down cell wall material and inactivate harmful cellular enzymes and chemicals Signup and view all the answers

What is the role of EDTA in the CTAB extraction buffer?

To chelate magnesium ions and inactivate DNAse Signup and view all the answers

What is the effect of high ionic strength solutions on CTAB?

CTAB forms complexes with proteins Signup and view all the answers

Why is NaCl added to the CTAB extraction buffer at concentrations of more than 0.5 M?

To remove polysaccharides from the DNA extract Signup and view all the answers

What is the purpose of using CTAB in DNA extraction from plant cells?

To precipitate nucleic acids and acidic polysaccharides Signup and view all the answers

What is the advantage of using CTAB in DNA extraction from organisms that produce large quantities of polysaccharides?

It is useful for purification of nucleic acid Signup and view all the answers

What is the purpose of adding 0.54 volume of ice-cold isopropanol to the sample?

To precipitate the DNA and remove salt Signup and view all the answers

What is the primary function of the 70% ethanol wash step in DNA extraction?

To remove salts and other contaminants from the DNA sample Signup and view all the answers

What is the purpose of adding RNase to the sample during DNA extraction?

To degrade any RNA present in the sample Signup and view all the answers

What is the primary function of the centrifugation step at 10,000 rpm for 10 minutes?

To separate the aqueous and chloroform phases Signup and view all the answers

What is the composition of the buffer used to resuspend the DNA pellet?

1X TE buffer or RNase-free H2O Signup and view all the answers

Why is it important to leave some of the upper phase behind when transferring the aqueous layer to another tube?

To prevent the pickup of any interphase or lower phase Signup and view all the answers

DNA Extraction Process

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