Nucleic Acid Electrophoresis Quiz
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Questions and Answers

What is the purpose of nucleic acid electrophoresis?

  • To separate DNA or RNA fragments by size and reactivity (correct)
  • To measure the concentration of nucleic acids
  • To determine the sequence of nucleic acid molecules
  • To identify the types of nucleic acids present

    • Why do nucleic acid molecules migrate toward the anode during electrophoresis?

  • Due to the electric field inducing a random movement
  • Due to the presence of positively charged ions in the gel
  • Due to their attraction to the gel medium
  • Due to their negatively charged sugar-phosphate backbone (correct)

    • How does the size of a nucleic acid molecule affect its mobility during electrophoresis?

  • Smaller fragments migrate faster due to less resistance (correct)
  • Larger fragments migrate faster due to more charge
  • Smaller fragments migrate slower due to more resistance
  • Size does not affect mobility in electrophoresis

    • What happens after the voltage is removed in nucleic acid electrophoresis?

    <p>The fragmentation gradient is analyzed</p> Signup and view all the answers

    For larger separations between similar sized fragments in nucleic acid electrophoresis, what can be adjusted?

    <p>Either the voltage or run time</p> Signup and view all the answers

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