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Questions and Answers
What is the purpose of nucleic acid electrophoresis?
What is the purpose of nucleic acid electrophoresis?
- To separate DNA or RNA fragments by size and reactivity (correct)
- To measure the concentration of nucleic acids
- To determine the sequence of nucleic acid molecules
- To identify the types of nucleic acids present
Why do nucleic acid molecules migrate toward the anode during electrophoresis?
Why do nucleic acid molecules migrate toward the anode during electrophoresis?
- Due to the electric field inducing a random movement
- Due to the presence of positively charged ions in the gel
- Due to their attraction to the gel medium
- Due to their negatively charged sugar-phosphate backbone (correct)
How does the size of a nucleic acid molecule affect its mobility during electrophoresis?
How does the size of a nucleic acid molecule affect its mobility during electrophoresis?
- Smaller fragments migrate faster due to less resistance (correct)
- Larger fragments migrate faster due to more charge
- Smaller fragments migrate slower due to more resistance
- Size does not affect mobility in electrophoresis
What happens after the voltage is removed in nucleic acid electrophoresis?
What happens after the voltage is removed in nucleic acid electrophoresis?
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For larger separations between similar sized fragments in nucleic acid electrophoresis, what can be adjusted?
For larger separations between similar sized fragments in nucleic acid electrophoresis, what can be adjusted?
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