12 Questions
What is the primary basis for separating nucleic acid molecules in gel electrophoresis?
Size
What is the range of DNA fragment sizes that can be separated using agarose?
A few hundred base pairs to about 20 kb
What is the preferred gel material for separating smaller DNA fragments?
Polyacrylamide
What is the original thought about DNA movement through the gel during electrophoresis?
Like a snake
What is the mechanism responsible for the separation of DNA molecules by molecular weight during gel electrophoresis?
Not well understood
What replaced velocity sedimentation in sucrose gradients for monitoring the progress of DNA molecule experiments?
Gel electrophoresis
What happens to DNA molecules when they are subjected to an applied field?
They stretch in the direction of the applied field and then contract into dense balls
What is the approximate size of DNA molecules that can be separated without pulsed electrical fields?
20 kb
What is the effect of increasing the pore size of the gel in DNA separation?
Larger DNA molecules can pass through
What is the purpose of changing the orientation of the electric field in PFGE?
To cause the DNA molecules to realign their axis and migrate in a new direction
What is the advantage of using CHEF electrophoresis over PFGE?
CHEF produces straight lines of migration, making analysis easier
What is the relationship between the migration rate of DNA molecules and their molecular weights?
Migration rate is inversely proportional to the logarithms of their molecular weights
Test your knowledge on gel electrophoresis, a laboratory technique used to separate and analyze nucleic acid molecules based on their size. Learn about its applications in DNA purification and analysis. Discover how it has replaced traditional methods like velocity sedimentation.
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