Gel Electrophoresis in Nucleic Acid Analysis
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Questions and Answers

What is the primary basis for separating nucleic acid molecules in gel electrophoresis?

  • Shape
  • Molecular weight
  • Size (correct)
  • Charge

    • What is the range of DNA fragment sizes that can be separated using agarose?

  • A few hundred base pairs to about 10 kb
  • A few hundred base pairs to about 20 kb (correct)
  • A few thousand base pairs to about 100 kb
  • A few thousand base pairs to about 50 kb

    • What is the preferred gel material for separating smaller DNA fragments?

  • Polyacrylamide (correct)
  • Sucrose
  • Agarose
  • Acrylamide

    • What is the original thought about DNA movement through the gel during electrophoresis?

    <p>Like a snake</p> Signup and view all the answers

    What is the mechanism responsible for the separation of DNA molecules by molecular weight during gel electrophoresis?

    <p>Not well understood</p> Signup and view all the answers

    What replaced velocity sedimentation in sucrose gradients for monitoring the progress of DNA molecule experiments?

    <p>Gel electrophoresis</p> Signup and view all the answers

    What happens to DNA molecules when they are subjected to an applied field?

    <p>They stretch in the direction of the applied field and then contract into dense balls</p> Signup and view all the answers

    What is the approximate size of DNA molecules that can be separated without pulsed electrical fields?

    <p>20 kb</p> Signup and view all the answers

    What is the effect of increasing the pore size of the gel in DNA separation?

    <p>Larger DNA molecules can pass through</p> Signup and view all the answers

    What is the purpose of changing the orientation of the electric field in PFGE?

    <p>To cause the DNA molecules to realign their axis and migrate in a new direction</p> Signup and view all the answers

    What is the advantage of using CHEF electrophoresis over PFGE?

    <p>CHEF produces straight lines of migration, making analysis easier</p> Signup and view all the answers

    What is the relationship between the migration rate of DNA molecules and their molecular weights?

    <p>Migration rate is inversely proportional to the logarithms of their molecular weights</p> Signup and view all the answers

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