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Questions and Answers
What is the primary basis for separating nucleic acid molecules in gel electrophoresis?
What is the range of DNA fragment sizes that can be separated using agarose?
What is the preferred gel material for separating smaller DNA fragments?
What is the original thought about DNA movement through the gel during electrophoresis?
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What is the mechanism responsible for the separation of DNA molecules by molecular weight during gel electrophoresis?
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What replaced velocity sedimentation in sucrose gradients for monitoring the progress of DNA molecule experiments?
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What happens to DNA molecules when they are subjected to an applied field?
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What is the approximate size of DNA molecules that can be separated without pulsed electrical fields?
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What is the effect of increasing the pore size of the gel in DNA separation?
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What is the purpose of changing the orientation of the electric field in PFGE?
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What is the advantage of using CHEF electrophoresis over PFGE?
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What is the relationship between the migration rate of DNA molecules and their molecular weights?
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