Podcast
Questions and Answers
What is the primary advantage of capillary electrophoresis over gel electrophoresis?
What is the primary advantage of capillary electrophoresis over gel electrophoresis?
- Increased sensitivity and immediate detection
- Decreased runtime and preparation time
- Automatic analysis of results by software
- All of the above (correct)
What is the purpose of a buffer system in electrophoresis?
What is the purpose of a buffer system in electrophoresis?
- To carry the current and protect the samples
- To maintain the pH of the solution
- To prevent severe fluctuations in the pH of the gel
- All of the above (correct)
Which of the following is a characteristic of a buffered solution?
Which of the following is a characteristic of a buffered solution?
- The buffer concentration must be low to provide sufficient acidic and basic forms
- The addition of acid or base will significantly affect the pH
- The buffer does not protect sample molecules from damage
- The pH remains constant as the buffer molecules take up or release protons (correct)
What is the main disadvantage of capillary electrophoresis?
What is the main disadvantage of capillary electrophoresis?
How does a buffer maintain the pH of a solution near its pKa?
How does a buffer maintain the pH of a solution near its pKa?
What is the role of buffer ions in the electrophoresis process?
What is the role of buffer ions in the electrophoresis process?
What is the purpose of inserting a comb into the gel in gel electrophoresis?
What is the purpose of inserting a comb into the gel in gel electrophoresis?
Why is it crucial to keep the thickness of the gel and the volume of the buffer constant in gel electrophoresis?
Why is it crucial to keep the thickness of the gel and the volume of the buffer constant in gel electrophoresis?
What will happen if contact is made with the gel buffer while the current is on in gel electrophoresis?
What will happen if contact is made with the gel buffer while the current is on in gel electrophoresis?
How does the charge/mass ratio of sample molecules affect their migration in gel electrophoresis?
How does the charge/mass ratio of sample molecules affect their migration in gel electrophoresis?
What happens to the gel after the comb is removed and it is placed into the gel box in gel electrophoresis?
What happens to the gel after the comb is removed and it is placed into the gel box in gel electrophoresis?
Why are gel baths designed to stop the current when a protective cover is removed from the gel bath?
Why are gel baths designed to stop the current when a protective cover is removed from the gel bath?
What is the role of methanol and acetic acid after electrophoresis on the sample?
What is the role of methanol and acetic acid after electrophoresis on the sample?
How does silver staining work under optimal pH conditions?
How does silver staining work under optimal pH conditions?
What is the main difference between silver diamine and silver nitrate procedures?
What is the main difference between silver diamine and silver nitrate procedures?
Which dye sits on the minor groove of the double helix for double-stranded DNA staining?
Which dye sits on the minor groove of the double helix for double-stranded DNA staining?
What is a disadvantage of SYBR gold dye?
What is a disadvantage of SYBR gold dye?
How does SYBR green differ from EtBr in terms of binding to DNA bases?
How does SYBR green differ from EtBr in terms of binding to DNA bases?
What is the impact of raising the buffer concentration on gel stability?
What is the impact of raising the buffer concentration on gel stability?
Which ions move more quickly through the gel?
Which ions move more quickly through the gel?
Why are Tris base and Borate preferred for maintaining constant pH during electrophoresis?
Why are Tris base and Borate preferred for maintaining constant pH during electrophoresis?
Which buffer is prone to precipitation and could result in localized distortions in DNA electrophoresis?
Which buffer is prone to precipitation and could result in localized distortions in DNA electrophoresis?
What other characteristics, besides pKa and charge, should be considered when choosing a buffer?
What other characteristics, besides pKa and charge, should be considered when choosing a buffer?
How does an increase in buffer concentration impact the conductivity of the electrophoresis system?
How does an increase in buffer concentration impact the conductivity of the electrophoresis system?
What is the main reason that the nucleic acid should be completely denatured (single stranded) before separation?
What is the main reason that the nucleic acid should be completely denatured (single stranded) before separation?
What is the primary purpose of using fluorescent labels on the nucleic acids?
What is the primary purpose of using fluorescent labels on the nucleic acids?
What is the main advantage of using a capillary in the electrophoresis process?
What is the main advantage of using a capillary in the electrophoresis process?
Which of the following is NOT a common forensic application of the capillary electrophoresis technique described in the text?
Which of the following is NOT a common forensic application of the capillary electrophoresis technique described in the text?
How does the electrokinetic injection process work to draw the sample into the capillary?
How does the electrokinetic injection process work to draw the sample into the capillary?
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